Novel phage display-derived recombinant antibodies recognizing both MPT64 native and mutant (63-bp deletion) are promising tools for tuberculosis diagnosis

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dc.contributorLab. Biofármacospt_BR
dc.contributor.authorMagalhães, Carolina Georgpt_BR
dc.contributor.authorMoreira, Gustavo Marçal Schmidt Garciapt_BR
dc.contributor.authorFerreira, Marcos Roberto Alvespt_BR
dc.contributor.authorSantos, Lucas Moreira dospt_BR
dc.contributor.authorFinger, Paula Fonsecapt_BR
dc.contributor.authorRamos, Daniela Fernandespt_BR
dc.contributor.authorSilva, Pedro Eduardo Almeida dapt_BR
dc.contributor.authorHust, Michaelpt_BR
dc.contributor.authorConceição, Fabricio Rochedopt_BR
dc.date.accessioned2021-07-27T17:43:36Z-
dc.date.available2021-07-27T17:43:36Z-
dc.date.issued2021pt_BR
dc.identifier.citationMagalhães CG, Moreira GMSG, Ferreira MRA, Santos LM, Finger PF, Ramos DF, et al. Novel phage display-derived recombinant antibodies recognizing both MPT64 native and mutant (63-bp deletion) are promising tools for tuberculosis diagnosis. Biologicals. 2021 July;in press. doi:10.1016/j.biologicals.2021.07.002.pt_BR
dc.identifier.urihttps://repositorio.butantan.gov.br/handle/butantan/3893-
dc.description.abstractTuberculosis (TB) is one of the top 10 causes of death in humans worldwide. The most important causative agents of TB are bacteria from the Mycobacterium tuberculosis complex (MTC), although nontuberculous mycobacteria (NTM) can also cause similar infections. The ability to identify and differentiate MTC isolates from NTM is important for the selection of the correct antimicrobial therapy. Immunochromatographic assays with antibodies anti-MPT64 allow differentiation between MTC and NTM since the MPT64 protein is specific from MTC. However, studies reported false-negative results mainly due to mpt64 63-bp deletion. Considering this drawback, we selected seven human antibody fragments against MPT64 by phage display and produced them as scFv-Fc. Three antibodies reacted with rMPT64 mutant (63-bp deletion) protein and native MPT64 from M. tuberculosis H37Rv in ELISA and Western blot. These antibodies are new biological tools with the potential for the development of TB diagnosis helping to overcome limitations of the MPT64-based immunochromatographic tests currently available.pt_BR
dc.description.sponsorshipCoordenação de Aperfeiçoamento de Pessoal de Nível Superior (CAPES)pt_BR
dc.description.sponsorshipFundação de Amparo à pesquisa do Estado do Rio Grande do Sul (FAPERGS)pt_BR
dc.description.sponsorshipConselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq)pt_BR
dc.language.isoEnglishpt_BR
dc.relation.ispartofBiologicalspt_BR
dc.rightsAcesso restritopt_BR
dc.titleNovel phage display-derived recombinant antibodies recognizing both MPT64 native and mutant (63-bp deletion) are promising tools for tuberculosis diagnosispt_BR
dc.typeArticlept_BR
dc.identifier.doi10.1016/j.biologicals.2021.07.002pt_BR
dc.identifier.urlhttps://doi.org/10.1016/j.biologicals.2021.07.002pt_BR
dc.contributor.externalUniversidade Federal de Pelotaspt_BR
dc.contributor.externalTechnische Universität Braunschweigpt_BR
dc.contributor.externalUniversidade Federal do Rio Grande (FURG)pt_BR
dc.subject.keywordMycobacterium tuberculosis complexpt_BR
dc.subject.keywordMPT64 mutationpt_BR
dc.subject.keywordNontuberculous mycobacteria (NTM)pt_BR
dc.subject.keywordDiagnosis M.tbpt_BR
dc.subject.keywordMonoclonal antibodiespt_BR
dc.relation.ispartofabbreviatedBiologicalspt_BR
dc.identifier.citationabntin press, jul. 2021pt_BR
dc.identifier.citationvancouver2021 July;in presspt_BR
dc.contributor.butantanMagalhães, Carolina Georg|:Desvinculado|:Lab. Biofármacospt_BR
dc.sponsorship.butantanCoordenação de Aperfeiçoamento de Pessoal de Nível Superior (CAPES)¦¦pt_BR
dc.sponsorship.butantanFundação de Amparo à pesquisa do Estado do Rio Grande do Sul (FAPERGS)¦¦pt_BR
dc.sponsorship.butantanConselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq)¦¦pt_BR
dc.identifier.bvsccBR78.1pt_BR
dc.identifier.bvsdbIBProdpt_BR
dc.description.dbindexedYespt_BR
item.openairetypeArticle-
item.fulltextCom Texto completo-
item.grantfulltextembargo_29990101-
item.languageiso639-1English-
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