AeMOPE-1, a novel salivary peptide from Aedes aegypti, selectively modulates activation of murine macrophages and ameliorates experimental Colitis

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dc.contributorLaboratório de Toxinologia Aplicadapt_BR
dc.contributor.authorLara, Priscila G.pt_BR
dc.contributor.authorEsteves, Elianept_BR
dc.contributor.authorSales-Campos, Helioswiltonpt_BR
dc.contributor.authorAssis, Josiane B.pt_BR
dc.contributor.authorHenrique, Maressa O.pt_BR
dc.contributor.authorBarros, Michele S.pt_BR
dc.contributor.authorS. Neto, Leilapt_BR
dc.contributor.authorSilva Junior, Pedro Ismael dapt_BR
dc.contributor.authorMartins, Joilson O.pt_BR
dc.contributor.authorCardoso, Cristina R. B.pt_BR
dc.contributor.authorRibeiro, José M. C.pt_BR
dc.contributor.authorSá-Nunes, Andersonpt_BR
dc.date.accessioned2021-08-19T17:53:35Z-
dc.date.available2021-08-19T17:53:35Z-
dc.date.issued2021pt_BR
dc.identifier.citationLara PG., Esteves E, Sales-Campos H, Assis JB., Henrique MO., Barros MS., et al. AeMOPE-1, a novel salivary peptide from Aedes aegypti, selectively modulates activation of murine macrophages and ameliorates experimental Colitis. Front. Immunol.. 2021 July;12:681671. doi:10.3389/fimmu.2021.681671.pt_BR
dc.identifier.urihttps://repositorio.butantan.gov.br/handle/butantan/3918-
dc.description.abstractThe sialotranscriptomes of Aedes aegypti revealed a transcript overexpressed in female salivary glands that codes a mature 7.8 kDa peptide. The peptide, specific to the Aedes genus, has a unique sequence, presents a putative secretory nature and its function is unknown. Here, we confirmed that the peptide is highly expressed in the salivary glands of female mosquitoes when compared to the salivary glands of males, and its secretion in mosquito saliva is able to sensitize the vertebrate host by inducing the production of specific antibodies. The synthetic version of the peptide downmodulated nitric oxide production by activated peritoneal murine macrophages. The fractionation of a Ae. aegypti salivary preparation revealed that the fractions containing the naturally secreted peptide reproduced the nitric oxide downmodulation. The synthetic peptide also selectively interfered with cytokine production by murine macrophages, inhibiting the production of IL-6, IL-12p40 and CCL2 without affecting TNF-α or IL-10 production. Likewise, intracellular proteins associated with macrophage activation were also distinctively modulated: while iNOS and NF-κB p65 expression were diminished, IκBα and p38 MAPK expression did not change in the presence of the peptide. The anti-inflammatory properties of the synthetic peptide were tested in vivo on a dextran sulfate sodium-induced colitis model. The therapeutic administration of the Ae. aegypti peptide reduced the leukocytosis, macrophage activity and nitric oxide levels in the gut, as well as the expression of cytokines associated with the disease, resulting in amelioration of its clinical signs. Given its biological properties in vitro and in vivo, the molecule was termed Aedes-specific MOdulatory PEptide (AeMOPE-1). Thus, AeMOPE-1 is a novel mosquito-derived immunobiologic with potential to treat immune-mediated disorders.pt_BR
dc.description.sponsorship(FAPESP) Fundação de Amparo à Pesquisa do Estado de São Paulopt_BR
dc.description.sponsorship(CNPq) Conselho Nacional de Desenvolvimento Científico e Tecnológicopt_BR
dc.description.sponsorship(USP) Universidade de São Paulopt_BR
dc.description.sponsorship(CAPES) Coordenação de Aperfeiçoamento de Pessoal de Nível Superiorpt_BR
dc.description.sponsorship(NIAID) National Institute of Allergy and Infectious Diseasespt_BR
dc.format.extent681671pt_BR
dc.language.isoEnglishpt_BR
dc.relation.ispartofFrontiers in Immunologypt_BR
dc.rightsOpen accesspt_BR
dc.rights.urihttps://creativecommons.org/licenses/by/4.0/pt_BR
dc.titleAeMOPE-1, a novel salivary peptide from Aedes aegypti, selectively modulates activation of murine macrophages and ameliorates experimental Colitispt_BR
dc.typeArticlept_BR
dc.rights.licenseCC BYpt_BR
dc.identifier.doi10.3389/fimmu.2021.681671pt_BR
dc.identifier.urlhttps://doi.org/10.3389/fimmu.2021.681671pt_BR
dc.contributor.external(USP) Universidade de São Paulopt_BR
dc.contributor.external(NIAID) National Institute of Allergy and Infectious Diseasespt_BR
dc.contributor.external(INCT - EM) Instituto Nacional de Ciência e Tecnologia em Entomologia Molecularpt_BR
dc.contributor.externalConselho Nacional de Desenvolvimento Científico e Tecnológico (CNPQ)pt_BR
dc.contributor.externalNational Institutes of Health (NIH)pt_BR
dc.identifier.citationvolume12pt_BR
dc.subject.keywordAedes aegyptipt_BR
dc.subject.keywordAeMOPE-1pt_BR
dc.subject.keywordmacrophagespt_BR
dc.subject.keywordimmunomodulationpt_BR
dc.subject.keywordexperimental colitispt_BR
dc.relation.ispartofabbreviatedFront. Immunol.pt_BR
dc.identifier.citationabntv. 12, 681671, jul. 2021pt_BR
dc.identifier.citationvancouver2021 July;12:681671pt_BR
dc.contributor.butantanSilva Junior, Pedro Ismael da|:Pesquisador|:Laboratório de Toxinologia Aplicadapt_BR
dc.sponsorship.butantan(FAPESP) Fundação de Amparo à Pesquisa do Estado de São Paulo¦¦2009/09892-6pt_BR
dc.sponsorship.butantan(FAPESP) Fundação de Amparo à Pesquisa do Estado de São Paulo¦¦2015/22934-0pt_BR
dc.sponsorship.butantan(FAPESP) Fundação de Amparo à Pesquisa do Estado de São Paulo¦¦2013/07467-1pt_BR
dc.sponsorship.butantan(FAPESP) Fundação de Amparo à Pesquisa do Estado de São Paulo¦¦2020/03175-0pt_BR
dc.sponsorship.butantan(FAPESP) Fundação de Amparo à Pesquisa do Estado de São Paulo¦¦2013/00740-4pt_BR
dc.sponsorship.butantan(CNPq) Conselho Nacional de Desenvolvimento Científico e Tecnológico¦¦465678/2014-9pt_BR
dc.sponsorship.butantan(CNPq) Conselho Nacional de Desenvolvimento Científico e Tecnológico¦¦310174/2016-3pt_BR
dc.sponsorship.butantan(CNPq) Conselho Nacional de Desenvolvimento Científico e Tecnológico¦¦311204/2018-0pt_BR
dc.sponsorship.butantan(CNPq) Conselho Nacional de Desenvolvimento Científico e Tecnológico¦¦472744/2012-7pt_BR
dc.sponsorship.butantan(CNPq) Conselho Nacional de Desenvolvimento Científico e Tecnológico¦¦310993/2020-2pt_BR
dc.sponsorship.butantan(CNPq) Conselho Nacional de Desenvolvimento Científico e Tecnológico¦¦309583/2019-5pt_BR
dc.sponsorship.butantanUniversidade de São Paulo (USP)¦¦12.1.17661.1.7pt_BR
dc.sponsorship.butantan(CAPES) Coordenação de Aperfeiçoamento de Pessoal de Nível Superior¦¦001pt_BR
dc.sponsorship.butantanNational Institute of Allergy and Infectious Diseases (NIAID)¦¦Z01 AI000810-20pt_BR
dc.identifier.bvsccBR78.1pt_BR
dc.identifier.bvsdbIBProdpt_BR
dc.description.dbindexedYespt_BR
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