Bothrops Jararaca snake venom modulates key cancer-related proteins in breast tumor cell lines

Full metadata record
DC FieldValueLanguage
dc.contributorLETA - Laboratório de Toxinologia Aplicadapt_BR
dc.contributorCeTICS - Centro de Toxinas, Resposta-imune e Sinalização Celularpt_BR
dc.contributorPrograma de Pós-Graduação em Ciências – Toxinologia (PPGTox)pt_BR
dc.contributor.authorKisaki, Carolina Yukikopt_BR
dc.contributor.authorArcos, Stephanie Santos Suehiropt_BR
dc.contributor.authorMontoni, Fabiopt_BR
dc.contributor.authorSantos, Wellington da Silvapt_BR
dc.contributor.authorCalacina, Hamida Macêdopt_BR
dc.contributor.authorLima, Ismael Feitosapt_BR
dc.contributor.authorCajado-Carvalho, Danielapt_BR
dc.contributor.authorFerro, Emer Suavinhopt_BR
dc.contributor.authorNishiyama Junior, Milton Yutakapt_BR
dc.contributor.authorIwai, Leo Keipt_BR
dc.date.accessioned2021-09-03T15:36:30Z-
dc.date.available2021-09-03T15:36:30Z-
dc.date.issued2021pt_BR
dc.identifier.citationKisaki CY, Arcos SSS, Montoni F, Santos WS, Calacina HM, Lima IF, et al. Bothrops Jararaca snake venom modulates key cancer-related proteins in breast tumor cell lines. Toxins. 2021 July;13(8):519. doi:10.3390/toxins13080519.pt_BR
dc.identifier.urihttps://repositorio.butantan.gov.br/handle/butantan/3919-
dc.description.abstractCancer is characterized by the development of abnormal cells that divide in an uncontrolled way and may spread into other tissues where they may infiltrate and destroy normal body tissue. Several previous reports have described biochemical anti-tumorigenic properties of crude snake venom or its components, including their capability of inhibiting cell proliferation and promoting cell death. However, to the best of our knowledge, there is no work describing cancer cell proteomic changes following treatment with snake venoms. In this work we describe the quantitative changes in proteomics of MCF7 and MDA-MB-231 breast tumor cell lines following treatment with Bothrops jararaca snake venom, as well as the functional implications of the proteomic changes. Cell lines were treated with sub-toxic doses at either 0.63 μg/mL (low) or 2.5 μg/mL (high) of B. jararaca venom for 24 h, conditions that cause no cell death per se. Proteomics analysis was conducted on a nano-scale liquid chromatography coupled on-line with mass spectrometry (nLC-MS/MS). More than 1000 proteins were identified and evaluated from each cell line treated with either the low or high dose of the snake venom. Protein profiling upon venom treatment showed differential expression of several proteins related to cancer cell metabolism, immune response, and inflammation. Among the identified proteins we highlight histone H3, SNX3, HEL-S-156an, MTCH2, RPS, MCC2, IGF2BP1, and GSTM3. These data suggest that sub-toxic doses of B. jararaca venom have potential to modulate cancer-development related protein targets in cancer cells. This work illustrates a novel biochemical strategy to identify therapeutic targets against cancer cell growth and survival.pt_BR
dc.description.sponsorshipFundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)pt_BR
dc.description.sponsorshipWeizmann Institute of Sciencept_BR
dc.description.sponsorshipConselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq)pt_BR
dc.description.sponsorshipCoordenação de Aperfeiçoamento de Pessoal de Nível Superior (CAPES)pt_BR
dc.description.sponsorshipFundação Butantanpt_BR
dc.format.extent519pt_BR
dc.language.isoEnglishpt_BR
dc.relation.ispartofToxinspt_BR
dc.rightsOpen Accesspt_BR
dc.titleBothrops Jararaca snake venom modulates key cancer-related proteins in breast tumor cell linespt_BR
dc.typeArticlept_BR
dc.identifier.doi10.3390/toxins13080519pt_BR
dc.identifier.urlhttps://doi.org/10.3390/toxins13080519pt_BR
dc.contributor.externalUniversidade de São Paulo (USP)pt_BR
dc.identifier.citationvolume13pt_BR
dc.identifier.citationissue8pt_BR
dc.subject.keywordmass spectrometrypt_BR
dc.subject.keywordproteomept_BR
dc.subject.keywordsnake venompt_BR
dc.subject.keywordBothrops jararacapt_BR
dc.subject.keywordbreast cancerpt_BR
dc.relation.ispartofabbreviatedToxinspt_BR
dc.identifier.citationabntv. 13, n. 8, 519, jul. 2021pt_BR
dc.identifier.citationvancouver2021 July;13(8):519pt_BR
dc.contributor.butantanKisaki, Carolina Yukiko|:Aluno Egresso|:LETA - Laboratório de Toxinologia Aplicada:CeTICS - Centro de Toxinas, Resposta-imune e Sinalização Celular|:PrimeiroAutorpt_BR
dc.contributor.butantanArcos, Stephanie Santos Suehiro|:Desvinculado|:LETA - Laboratório de Toxinologia Aplicada:CeTICS - Centro de Toxinas, Resposta-imune e Sinalização Celularpt_BR
dc.contributor.butantanMontoni, Fabio|:Aluno Egresso|:LETA - Laboratório de Toxinologia Aplicada:CeTICS - Centro de Toxinas, Resposta-imune e Sinalização Celularpt_BR
dc.contributor.butantanSantos, Wellington da Silva|:Aluno|:LETA - Laboratório de Toxinologia Aplicada:CeTICS - Centro de Toxinas, Resposta-imune e Sinalização Celularpt_BR
dc.contributor.butantanCalacina, Hamida Macêdo|:Desvinculado|:LETA - Laboratório de Toxinologia Aplicada:CeTICS - Centro de Toxinas, Resposta-imune e Sinalização Celularpt_BR
dc.contributor.butantanLima, Ismael Feitosa|:Outros|:LETA - Laboratório de Toxinologia Aplicada:CeTICS - Centro de Toxinas, Resposta-imune e Sinalização Celularpt_BR
dc.contributor.butantanCajado-Carvalho, Daniela|:Desvinculado|:LETA - Laboratório de Toxinologia Aplicada:CeTICS - Centro de Toxinas, Resposta-imune e Sinalização Celularpt_BR
dc.contributor.butantanNishiyama Junior, Milton Yutaka|:Pesquisador|:LETA - Laboratório de Toxinologia Aplicada:CeTICS - Centro de Toxinas, Resposta-imune e Sinalização Celular|:Autor de correspondênciapt_BR
dc.contributor.butantanIwai, Leo Kei|:Pesquisador|:Laboratório Especial de Toxinologia Aplicada (LETA):Centro de Toxinas, Resposta-imune e Sinalização Celular (CeTICS)|:Autor de correspondênciapt_BR
dc.sponsorship.butantanFundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)¦¦2013/07467-1pt_BR
dc.sponsorship.butantanFundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)¦¦2016/04000-3pt_BR
dc.sponsorship.butantanFundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)¦¦2017/17943-6pt_BR
dc.sponsorship.butantanFundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)¦¦2017/06496-9pt_BR
dc.sponsorship.butantanWeizmann Institute of Science¦¦pt_BR
dc.sponsorship.butantanConselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq)¦¦302809/2016-3pt_BR
dc.sponsorship.butantanConselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq)¦¦88882.442313/2019-01pt_BR
dc.sponsorship.butantanCoordenação de Aperfeiçoamento de Pessoal de Nível Superior (CAPES)¦¦pt_BR
dc.sponsorship.butantanFundação Butantan¦¦pt_BR
dc.sponsorship.butantanUniversidade de São Paulo (USP)¦¦131408/2019-4pt_BR
dc.identifier.bvsccBR78.1pt_BR
dc.identifier.bvsdbIBProdpt_BR
dc.description.dbindexedYespt_BR
item.openairetypeArticle-
item.fulltextCom Texto completo-
item.grantfulltextembargo_29990101-
item.languageiso639-1English-
crisitem.author.dept#PLACEHOLDER_PARENT_METADATA_VALUE#-
crisitem.author.dept#PLACEHOLDER_PARENT_METADATA_VALUE#-
crisitem.author.dept#PLACEHOLDER_PARENT_METADATA_VALUE#-
crisitem.author.dept#PLACEHOLDER_PARENT_METADATA_VALUE#-
crisitem.author.dept#PLACEHOLDER_PARENT_METADATA_VALUE#-
crisitem.author.dept#PLACEHOLDER_PARENT_METADATA_VALUE#-
crisitem.author.dept#PLACEHOLDER_PARENT_METADATA_VALUE#-
crisitem.author.dept#PLACEHOLDER_PARENT_METADATA_VALUE#-
crisitem.author.dept#PLACEHOLDER_PARENT_METADATA_VALUE#-
crisitem.author.dept#PLACEHOLDER_PARENT_METADATA_VALUE#-
crisitem.author.orcid#PLACEHOLDER_PARENT_METADATA_VALUE#-
crisitem.author.orcid#PLACEHOLDER_PARENT_METADATA_VALUE#-
crisitem.author.orcid#PLACEHOLDER_PARENT_METADATA_VALUE#-
crisitem.author.orcid#PLACEHOLDER_PARENT_METADATA_VALUE#-
crisitem.author.orcid#PLACEHOLDER_PARENT_METADATA_VALUE#-
crisitem.author.orcid#PLACEHOLDER_PARENT_METADATA_VALUE#-
crisitem.author.orcid#PLACEHOLDER_PARENT_METADATA_VALUE#-
crisitem.author.orcid#PLACEHOLDER_PARENT_METADATA_VALUE#-
crisitem.author.orcid0000-0002-2410-0562-
crisitem.author.orcid0000-0002-1571-7763-
crisitem.author.parentorg#PLACEHOLDER_PARENT_METADATA_VALUE#-
crisitem.author.parentorg#PLACEHOLDER_PARENT_METADATA_VALUE#-
crisitem.author.parentorg#PLACEHOLDER_PARENT_METADATA_VALUE#-
crisitem.author.parentorg#PLACEHOLDER_PARENT_METADATA_VALUE#-
crisitem.author.parentorg#PLACEHOLDER_PARENT_METADATA_VALUE#-
crisitem.author.parentorg#PLACEHOLDER_PARENT_METADATA_VALUE#-
crisitem.journal.journalissn#PLACEHOLDER_PARENT_METADATA_VALUE#-
crisitem.journal.journaleissn#PLACEHOLDER_PARENT_METADATA_VALUE#-
Appears in Collections:Artigos de periódicos


Files in This Item:

Existing users please Login
toxins-13-00519-v2.pdf
Description:
Size: 2.64 MB
Format: Adobe PDF
Embargoed until January 1, 2999    Request a copy
Show simple item record

The access to the publications deposited in this repository respects the licenses from journals and publishers.