Application of CRISPR interference (CRISPRi) for gene silencing in pathogenic species of Leptospira

Full metadata record
DC FieldValueLanguage
dc.contributorLaboratório de Desenvolvimento de Vacinaspt_BR
dc.contributor.authorFernandes, L. G. V.pt_BR
dc.contributor.authorHornsby, R. L.pt_BR
dc.contributor.authorNascimento, Ana Lúcia Tabet Oller dopt_BR
dc.contributor.authorNally, J. E.pt_BR
dc.date.accessioned2021-09-17T12:56:36Z-
dc.date.available2021-09-17T12:56:36Z-
dc.date.issued2021pt_BR
dc.identifier.citationFernandes L.G.V., Hornsby R.L., Nascimento ALTO, Nally J.E.. Application of CRISPR interference (CRISPRi) for gene silencing in pathogenic species of Leptospira. Genetics. 2021 Aug;174:e62631. doi:10.3791/62631.pt_BR
dc.identifier.urihttps://repositorio.butantan.gov.br/handle/butantan/3933-
dc.description.abstractLeptospirosis is a global neglected zoonosis, responsible for at least 1 million cases per year and almost 60 thousand deaths. The disease is caused by pathogenic and virulent bacteria of the genus Leptospira, either by direct contact with the bacteria or indirectly by exposure to contaminated water or soil. Domestic and wild animals act as reservoir hosts of infection, shedding leptospires from colonized renal tubules of the kidney, via urine, into the environment. The generation of mutant strains of Leptospira is critical to evaluate and understand pathogenic mechanisms of infection. CRISPR interference (CRISPRi) has proven to be a straightforward, affordable, and specific tool for gene silencing in pathogenic Leptospira. Therefore, the methodological details of obtaining the plasmid constructs containing both dCas9 and guide RNA, delivery of plasmids to Leptospira by conjugation with the E. coli strain β2163, and transconjugant recovery and evaluation, will be described. In addition, the recently described Hornsby-Alt-Nally (HAN) media allows for the relatively rapid isolation and selection of mutant colonies on agar plates.pt_BR
dc.description.sponsorshipFundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)pt_BR
dc.format.extente62631pt_BR
dc.language.isoEnglishpt_BR
dc.relation.ispartofGeneticspt_BR
dc.rightsOpen accesspt_BR
dc.titleApplication of CRISPR interference (CRISPRi) for gene silencing in pathogenic species of Leptospirapt_BR
dc.typeArticlept_BR
dc.rights.licenseCC BY-NC-NDpt_BR
dc.identifier.doi10.3791/62631pt_BR
dc.identifier.urlhttps://doi.org/10.3791/62631pt_BR
dc.contributor.externalUnited States Department of Agriculture (USDA)pt_BR
dc.identifier.citationvolume174pt_BR
dc.relation.ispartofabbreviatedGeneticspt_BR
dc.identifier.citationabntv. 174, e62631, ago. 2021pt_BR
dc.identifier.citationvancouver2021 Aug;174:e62631pt_BR
dc.contributor.butantanNascimento, Ana Lúcia Tabet Oller do|:Pesquisador|:Laboratório de Desenvolvimento de Vacinaspt_BR
dc.sponsorship.butantanFundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)¦¦2014/50981-0pt_BR
dc.sponsorship.butantanFundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)¦¦2017/06731-8pt_BR
dc.sponsorship.butantanFundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)¦¦ 2019/20302-8pt_BR
dc.identifier.bvsccBR78.1pt_BR
dc.identifier.bvsdbIBProdpt_BR
dc.description.dbindexedYespt_BR
item.languageiso639-1English-
item.fulltextCom Texto completo-
item.openairetypeArticle-
item.grantfulltextopen-
crisitem.author.dept#PLACEHOLDER_PARENT_METADATA_VALUE#-
crisitem.author.dept#PLACEHOLDER_PARENT_METADATA_VALUE#-
crisitem.author.dept#PLACEHOLDER_PARENT_METADATA_VALUE#-
crisitem.author.dept#PLACEHOLDER_PARENT_METADATA_VALUE#-
crisitem.author.orcid#PLACEHOLDER_PARENT_METADATA_VALUE#-
crisitem.author.orcid#PLACEHOLDER_PARENT_METADATA_VALUE#-
crisitem.author.orcid0000-0003-4851-0870-
crisitem.author.orcid#PLACEHOLDER_PARENT_METADATA_VALUE#-
crisitem.author.parentorg#PLACEHOLDER_PARENT_METADATA_VALUE#-
crisitem.author.parentorg#PLACEHOLDER_PARENT_METADATA_VALUE#-
crisitem.author.parentorg#PLACEHOLDER_PARENT_METADATA_VALUE#-
crisitem.journal.journalissn#PLACEHOLDER_PARENT_METADATA_VALUE#-
crisitem.journal.journaleissn#PLACEHOLDER_PARENT_METADATA_VALUE#-
Appears in Collections:Artigos de periódicos


Files in This Item:

10.379162631.pdf
Description:
Size: 643.46 kB
Format: Adobe PDF
View/Open
Show simple item record

The access to the publications deposited in this repository respects the licenses from journals and publishers.