Genome-resolved metagenome and metatranscriptome analyses of thermophilic composting reveal key bacterial players and their metabolic interactions

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dc.contributor(LCC) Laboratório de Ciclo Celularpt_BR
dc.contributor.authorBraga, Lucas Palma Perezpt_BR
dc.contributor.authorPereira, Roberta Vercianopt_BR
dc.contributor.authorMartins, Layla Faragept_BR
dc.contributor.authorMoura, Livia Maria Silvapt_BR
dc.contributor.authorSanchez, Fabio Beltramept_BR
dc.contributor.authorPatané, José Salvatore Leisterpt_BR
dc.contributor.authorSilva, Aline Maria dapt_BR
dc.contributor.authorSetubal, João Carlospt_BR
dc.date.accessioned2021-10-01T15:21:57Z-
dc.date.available2021-10-01T15:21:57Z-
dc.date.issued2021pt_BR
dc.identifier.citationBraga LPP, Pereira RV, Martins LF, Moura LMS, Sanchez FB, Patané JSL, et al. Genome-resolved metagenome and metatranscriptome analyses of thermophilic composting reveal key bacterial players and their metabolic interactions. BMC Genomics. 2021 Sept;22:652. doi:10.1186/s12864-021-07957-9.pt_BR
dc.identifier.urihttps://repositorio.butantan.gov.br/handle/butantan/3946-
dc.description.abstractBackground Composting is an important technique for environment-friendly degradation of organic material, and is a microbe-driven process. Previous metagenomic studies of composting have presented a general description of the taxonomic and functional diversity of its microbial populations, but they have lacked more specific information on the key organisms that are active during the process. Results Here we present and analyze 60 mostly high-quality metagenome-assembled genomes (MAGs) recovered from time-series samples of two thermophilic composting cells, of which 47 are potentially new bacterial species; 24 of those did not have any hits in two public MAG datasets at the 95% average nucleotide identity level. Analyses of gene content and expressed functions based on metatranscriptome data for one of the cells grouped the MAGs in three clusters along the 99-day composting process. By applying metabolic modeling methods, we were able to predict metabolic dependencies between MAGs. These models indicate the importance of coadjuvant bacteria that do not carry out lignocellulose degradation but may contribute to the management of reactive oxygen species and with enzymes that increase bioenergetic efficiency in composting, such as hydrogenases and N2O reductase. Strong metabolic dependencies predicted between MAGs revealed key interactions relying on exchange of H+, NH3, O2 and CO2, as well as glucose, glutamate, succinate, fumarate and others, highlighting the importance of functional stratification and syntrophic interactions during biomass conversion. Our model includes 22 out of 49 MAGs recovered from one composting cell data. Based on this model we highlight that Rhodothermus marinus, Thermobispora bispora and a novel Gammaproteobacterium are dominant players in chemolithotrophic metabolism and cross-feeding interactions. Conclusions The results obtained expand our knowledge of the taxonomic and functional diversity of composting bacteria and provide a model of their dynamic metabolic interactions.pt_BR
dc.description.sponsorship(FAPESP) Fundação de Amparo à Pesquisa do Estado de São Paulopt_BR
dc.description.sponsorship(CAPES) Coordenação de Aperfeiçoamento de Pessoal de Nível Superiorpt_BR
dc.description.sponsorship(CNPq) Conselho Nacional de Desenvolvimento Científico e Tecnológicopt_BR
dc.format.extent652pt_BR
dc.language.isoEnglishpt_BR
dc.relation.ispartofBMC Genomicspt_BR
dc.rightsOpen accesspt_BR
dc.rights.urihttps://creativecommons.org/licenses/by/4.0/pt_BR
dc.titleGenome-resolved metagenome and metatranscriptome analyses of thermophilic composting reveal key bacterial players and their metabolic interactionspt_BR
dc.typeArticlept_BR
dc.rights.licenseCC BYpt_BR
dc.identifier.doi10.1186/s12864-021-07957-9pt_BR
dc.identifier.urlhttps://doi.org/10.1186/s12864-021-07957-9pt_BR
dc.contributor.external(USP) Universidade de São Paulopt_BR
dc.identifier.citationvolume22pt_BR
dc.subject.keywordMetagenome-assembled genomept_BR
dc.subject.keywordMicrobiomept_BR
dc.subject.keywordBiomass degradationpt_BR
dc.subject.keywordRhodothermus marinuspt_BR
dc.subject.keywordThermobispora bisporapt_BR
dc.relation.ispartofabbreviatedBMC Genomicspt_BR
dc.identifier.citationabntv. 22, 652, set. 2021pt_BR
dc.identifier.citationvancouver2021 Sept;22:652pt_BR
dc.contributor.butantanPatané, José Salvatore Leister|:Pesquisador|:LCC - Laboratório de Ciclo Celularpt_BR
dc.sponsorship.butantan(FAPESP) Fundação de Amparo à Pesquisa do Estado de São Paulo¦¦11/50870-6pt_BR
dc.sponsorship.butantan(FAPESP) Fundação de Amparo à Pesquisa do Estado de São Paulo¦¦18/19247-0pt_BR
dc.sponsorship.butantan(CAPES) Coordenação de Aperfeiçoamento de Pessoal de Nível Superior¦¦3385/2013pt_BR
dc.sponsorship.butantan(CNPq) Conselho Nacional de Desenvolvimento Científico e Tecnológico¦¦pt_BR
dc.identifier.bvsccBR78.1pt_BR
dc.identifier.bvsdbIBProdpt_BR
dc.description.dbindexedYespt_BR
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