Oral vaccination of piglets against Mycoplasma hyopneumoniae using silica SBA-15 as an adjuvant effectively reduced consolidation lung lesions at slaughter

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dc.contributor(LBI) Lab. Imunoquímicapt_BR
dc.contributor.authorMechler-Dreibi, Marina L.pt_BR
dc.contributor.authorAlmeida, Henrique M. S.pt_BR
dc.contributor.authorSonalio, Karinapt_BR
dc.contributor.authorMartines, Mariela A. C.pt_BR
dc.contributor.authorPetri, Fernando A. M.pt_BR
dc.contributor.authorZambotti, Beatriz B.pt_BR
dc.contributor.authorFerreira, Marcela M.pt_BR
dc.contributor.authorStorino, Gabriel Y.pt_BR
dc.contributor.authorMartins, Tereza S.pt_BR
dc.contributor.authorMontassier, Hélio Jpt_BR
dc.contributor.authorSant'anna, Osvaldo Augustopt_BR
dc.contributor.authorFantini, Márcia C. A.pt_BR
dc.contributor.authorOliveira, Luís Guilherme dept_BR
dc.date.accessioned2021-12-01T16:17:08Z-
dc.date.available2021-12-01T16:17:08Z-
dc.date.issued2021pt_BR
dc.identifier.citationMechler-Dreibi ML., Almeida HM.S., Sonalio K, Martines MA.C., Petri FA.M., Zambotti BB., et al. Oral vaccination of piglets against Mycoplasma hyopneumoniae using silica SBA-15 as an adjuvant effectively reduced consolidation lung lesions at slaughter. Sci. Rep. 2021 Nov;11:22377. doi:10.1038/s41598-021-01883-2.pt_BR
dc.identifier.urihttps://repositorio.butantan.gov.br/handle/butantan/3997-
dc.description.abstractMycoplasma (M.) hyopneumoniae is the main pathogen of porcine enzootic pneumonia (PEP). Its controlling is challenging, and requires alternative strategies. This study aimed to develop an oral vaccine against M. hyopneumoniae using a nanostructured mesoporous silica (SBA-15) as an adjuvant, and compare its effect with an intramuscular (IM) commercial vaccine (CV). Fifty 24 day-old M. hyopneumoniae-free piglets composed five equal groups for different immunization protocols, consisting of a CV and/or oral immunization (OI). Control piglets did not receive any form of immunization. All piglets were challenged with M. hyopneumoniae strain 232 on D49 by tracheal route. IgA antibody response in the respiratory tract, bacterial shedding and serum IgG were evaluated. The piglets were euthanized on 28 (D77) and 56 (D105) days post-infection. Lung lesions were macroscopically evaluated; lung fragments and bronchoalveolar fluid (BALF) were collected for estimation of bacterial loads by qPCR and/or histopathology examination. All immunization protocols induced reduction on Mycoplasma-like macroscopic lung lesions. IgA Ab responses anti-M. hyopneumoniae, the expression of IL-4 cytokine and a lower expression of IL-8 were induced by CV and OI vaccines, while IgG was induced only by CV. Oral immunization using silica as a carrier-adjuvant can be viable in controlling M. hyopneumoniae infection.pt_BR
dc.description.sponsorship(FAPESP) Fundação de Amparo à Pesquisa do Estado de São Paulopt_BR
dc.format.extent22377pt_BR
dc.language.isoEnglishpt_BR
dc.relation.ispartofScientific Reportspt_BR
dc.rightsOpen accesspt_BR
dc.rights.urihttps://creativecommons.org/licenses/by/4.0/pt_BR
dc.titleOral vaccination of piglets against Mycoplasma hyopneumoniae using silica SBA-15 as an adjuvant effectively reduced consolidation lung lesions at slaughterpt_BR
dc.typeArticlept_BR
dc.rights.licenseCC BYpt_BR
dc.identifier.doi10.1038/s41598-021-01883-2pt_BR
dc.identifier.urlttps://doi.org/10.1038/s41598-021-01883-2pt_BR
dc.contributor.external(UNESP) Universidade Estadual Paulista Júlio de Mesquita Filhopt_BR
dc.contributor.external(UNIFESP) Universidade Federal de São Paulopt_BR
dc.contributor.external(USP) Universidade de São Paulopt_BR
dc.identifier.citationvolume11pt_BR
dc.relation.ispartofabbreviatedSci Reppt_BR
dc.identifier.citationabntv. 11, 22377, nov. 2021pt_BR
dc.identifier.citationvancouver2021 Nov;11:22377pt_BR
dc.contributor.butantanSant'anna, Osvaldo Augusto|:Pesquisador|:Lab. Imunoquímicapt_BR
dc.sponsorship.butantan(FAPESP) Fundação de Amparo à Pesquisa do Estado de São Paulo¦¦2019/19710-4pt_BR
dc.sponsorship.butantan(FAPESP) Fundação de Amparo à Pesquisa do Estado de São Paulo¦¦2018/12742-5pt_BR
dc.sponsorship.butantan(FAPESP) Fundação de Amparo à Pesquisa do Estado de São Paulo¦¦2017/17844-8pt_BR
dc.identifier.bvsccBR78.1pt_BR
dc.identifier.bvsdbIBProdpt_BR
dc.description.dbindexedYespt_BR
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