Correlating biological activity to thermo-structural analysis of the interaction of CTX with synthetic models of macrophage membranes

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dc.contributorLab. Fisiopatologiapt_BR
dc.contributor.authorPimenta, Luciana de Araujopt_BR
dc.contributor.authorDuarte, Evandro L.pt_BR
dc.contributor.authorMuniz, Gabriel S. Vignolipt_BR
dc.contributor.authorPasqualoto, Kerly Fernanda Mesquitapt_BR
dc.contributor.authorFontes, Marcos Roberto de Mattospt_BR
dc.contributor.authorLamy, M. Teresapt_BR
dc.contributor.authorSampaio, Sandra Coccuzzopt_BR
dc.date.accessioned2021-12-14T19:23:19Z-
dc.date.available2021-12-14T19:23:19Z-
dc.date.issued2021pt_BR
dc.identifier.citationPimenta LA, Duarte EL., Muniz GS.V, Pasqualoto KFM, Fontes MRM, Lamy M.T, et al. Correlating biological activity to thermo-structural analysis of the interaction of CTX with synthetic models of macrophage membranes. Sci. Rep. 2021 Dec;11:23712. doi:10.1038/s41598-021-02552-0.pt_BR
dc.identifier.urihttps://repositorio.butantan.gov.br/handle/butantan/4029-
dc.description.abstractThe important pharmacological actions of Crotoxin (CTX) on macrophages, the main toxin in the venom of Crotalus durissus terrificus, and its important participation in the control of different pathophysiological processes, have been demonstrated. The biological activities performed by macrophages are related to signaling mediated by receptors expressed on the membrane surface of these cells or opening and closing of ion channels, generation of membrane curvature and pore formation. In the present work, the interaction of the CTX complex with the cell membrane of macrophages is studied, both using biological cells and synthetic lipid membranes to monitor structural alterations induced by the protein. Here we show that CTX can penetrate THP-1 cells and induce pores only in anionic lipid model membranes, suggesting that a possible access pathway for CTX to the cell is via lipids with anionic polar heads. Considering that the selectivity of the lipid composition varies in different tissues and organs of the human body, the thermostructural studies presented here are extremely important to open new investigations on the biological activities of CTX in different biological systems.pt_BR
dc.description.sponsorship(CNPq) Conselho Nacional de Desenvolvimento Científico e Tecnológicopt_BR
dc.description.sponsorship(INCT-FCx) Instituto Nacional de Ciência e Tecnologia de Fluidos Complexospt_BR
dc.description.sponsorship(FAPESP) Fundação de Amparo à Pesquisa do Estado de São Paulopt_BR
dc.format.extent23712pt_BR
dc.language.isoEnglishpt_BR
dc.relation.ispartofScientific Reportspt_BR
dc.rightsOpen accesspt_BR
dc.rights.urihttps://creativecommons.org/licenses/by/4.0/pt_BR
dc.titleCorrelating biological activity to thermo-structural analysis of the interaction of CTX with synthetic models of macrophage membranespt_BR
dc.typeArticlept_BR
dc.rights.licenseCC BYpt_BR
dc.identifier.doi10.1038/s41598-021-02552-0pt_BR
dc.identifier.urlhttps://doi.org/10.1038/s41598-021-02552-0pt_BR
dc.contributor.external(USP) Universidade de São Paulopt_BR
dc.contributor.external(UNESP) Universidade Estadual Paulista Júlio de Mesquita Filhopt_BR
dc.identifier.citationvolume11pt_BR
dc.relation.ispartofabbreviatedSci. Reppt_BR
dc.identifier.citationabntv. 11, 23712, dez. 2021pt_BR
dc.identifier.citationvancouver2021 Dec;11:23712pt_BR
dc.contributor.butantanPimenta, Luciana de Araujo|:Técnico|:Lab. Fisiopatologia|:PrimeiroAutor:pt_BR
dc.contributor.butantanSampaio, Sandra Coccuzzo|:Pesquisador:Docente Permanente PPGTox|:Lab. Fisiopatologiapt_BR
dc.sponsorship.butantan(CNPq) Conselho Nacional de Desenvolvimento Científico e Tecnológico¦¦158259/2018–1pt_BR
dc.sponsorship.butantan(CNPq) Conselho Nacional de Desenvolvimento Científico e Tecnológico¦¦302883/2017–7pt_BR
dc.sponsorship.butantan(CNPq) Conselho Nacional de Desenvolvimento Científico e Tecnológico¦¦465259/2014–6pt_BR
dc.sponsorship.butantan(CNPq) Conselho Nacional de Desenvolvimento Científico e Tecnológico¦¦301685/2017–7pt_BR
dc.sponsorship.butantan(INCT-FCx) Instituto Nacional de Ciência e Tecnologia de Fluidos Complexos¦¦pt_BR
dc.sponsorship.butantan(FAPESP) Fundação de Amparo à Pesquisa do Estado de São Paulo¦¦2017/11161–6pt_BR
dc.sponsorship.butantan(FAPESP) Fundação de Amparo à Pesquisa do Estado de São Paulo¦¦2014/50983–3pt_BR
dc.sponsorship.butantan(FAPESP) Fundação de Amparo à Pesquisa do Estado de São Paulo¦¦2017/11161–6pt_BR
dc.identifier.bvsccBR78.1pt_BR
dc.identifier.bvsdbIBProdpt_BR
dc.description.dbindexedYespt_BR
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