Molecular characterization and functional analysis of the nattectin-like toxin from the venomous fish thalassophryne maculosa

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dc.contributorLab. de Toxinologia Aplicadapt_BR
dc.contributorCeTICS - Centro de Toxinas, Resposta-imune e Sinalização Celularpt_BR
dc.contributorPrograma de Pós-Graduação em Ciências – Toxinologia (PPGTox)pt_BR
dc.contributorIniciação Científicapt_BR
dc.contributor.authorLopes-Ferreira, Monicapt_BR
dc.contributor.authorSosa-Rosales, Inespt_BR
dc.contributor.authorSilva Junior, Pedro Ismael dapt_BR
dc.contributor.authorConceicao, Katiapt_BR
dc.contributor.authorMaleski, Adolfo Luis Almeidapt_BR
dc.contributor.authorLima, Leticia Balanpt_BR
dc.contributor.authorDisner, Geonildo Rodrigopt_BR
dc.contributor.authorLima, Carlapt_BR
dc.date.accessioned2022-01-26T12:29:44Z-
dc.date.available2022-01-26T12:29:44Z-
dc.date.issued2021pt_BR
dc.identifier.citationLopes-Ferreira M, Sosa-Rosales I, Silva Junior PI, Conceicao K, Maleski ALA, Lima LB, et al. Molecular characterization and functional analysis of the nattectin-like toxin from the venomous fish thalassophryne maculosa. Toxins. 2021 Dec;14(1):2. doi:10.3390/toxins14010002.pt_BR
dc.identifier.urihttps://repositorio.butantan.gov.br/handle/butantan/4103-
dc.description.abstractTmC4-47.2 is a toxin with myotoxic activity found in the venom of Thalassophryne maculosa, a venomous fish commonly found in Latin America whose envenomation produces an injury characterized by delayed neutrophil migration, production of major pro-inflammatory cytokines, and necrosis at the wound site, as well as a specific systemic immune response. However, there are few studies on the protein structure and functions associated with it. Here, the toxin was identified from the crude venom by chromatography and protein purification systems. TmC4-47.2 shows high homology with the Nattectin from Thalassophryne nattereri venom, with 6 cysteines and QPD domain for binding to galactose. We confirm its hemagglutinating and microbicide abilities independent of carbohydrate binding, supporting its classification as a nattectin-like lectin. After performing the characterization of TmC4-47.2, we verified its ability to induce an increase in the rolling and adherence of leukocytes in cremaster post-capillary venules dependent on the α5β1 integrin. Finally, we could observe the inflammatory activity of TmC4-47.2 through the production of IL-6 and eotaxin in the peritoneal cavity with sustained recruitment of eosinophils and neutrophils up to 24 h. Together, our study characterized a nattectin-like protein from T. maculosa, pointing to its role as a molecule involved in the carbohydrate-independent agglutination response and modulation of eosinophilic and neutrophilic inflammation.pt_BR
dc.description.sponsorship(FAPESP) Fundação de Amparo à Pesquisa do Estado de São Paulopt_BR
dc.description.sponsorship(CAPES) Coordenação de Aperfeiçoamento de Pessoal de Nível Superiorpt_BR
dc.description.sponsorship(CNPq) Conselho Nacional de Desenvolvimento Científico e Tecnológicopt_BR
dc.format.extent2pt_BR
dc.language.isoEnglishpt_BR
dc.relation.ispartofToxinspt_BR
dc.rightsOpen accesspt_BR
dc.rights.urihttps://creativecommons.org/licenses/by/4.0/pt_BR
dc.titleMolecular characterization and functional analysis of the nattectin-like toxin from the venomous fish thalassophryne maculosapt_BR
dc.typeArticlept_BR
dc.rights.licenseCC BYpt_BR
dc.identifier.doi10.3390/toxins14010002pt_BR
dc.identifier.urlhttps://doi.org/10.3390/toxins14010002pt_BR
dc.contributor.external(UDO) Universidad de Orientept_BR
dc.contributor.external(UNIFESP) Universidade Federal de São Paulopt_BR
dc.identifier.citationvolume14pt_BR
dc.identifier.citationissue1pt_BR
dc.subject.keywordThalassophrynept_BR
dc.subject.keywordnattectinpt_BR
dc.subject.keywordreverse-phase HPLCpt_BR
dc.subject.keywordMALDI-ToFpt_BR
dc.subject.keywordhemagglutinating activitypt_BR
dc.subject.keywordantibacterial activitypt_BR
dc.subject.keywordinflammationpt_BR
dc.subject.keywordtoxinologypt_BR
dc.subject.keywordanimal toxinspt_BR
dc.relation.ispartofabbreviatedToxinspt_BR
dc.identifier.citationabntv. 14, n. 1, 2, dez. 2021pt_BR
dc.identifier.citationvancouver2021 Dec;14(1):2pt_BR
dc.contributor.butantanLopes-Ferreira, Monica|:Pesquisador|:Docente Permanente PPGTox|:Lab. de Toxinologia Aplicada:CeTICS - Centro de Toxinas, Resposta-imune e Sinalização Celular|:PrimeiroAutor:Autor de correspondênciapt_BR
dc.contributor.butantanSilva Junior, Pedro Ismael da|:Pesquisador|:Lab. de Toxinologia Aplicada:CeTICS - Centro de Toxinas, Resposta-imune e Sinalização Celularpt_BR
dc.contributor.butantanMaleski, Adolfo Luis Almeida|:Aluno|:Lab. de Toxinologia Aplicada:CeTICS - Centro de Toxinas, Resposta-imune e Sinalização Celular:Programa de Pós-Graduação em Ciências – Toxinologia (PPGTox)pt_BR
dc.contributor.butantanLima, Leticia Balan|:Aluno|:Lab. de Toxinologia Aplicada:CeTICS - Centro de Toxinas, Resposta-imune e Sinalização Celular:Iniciação Científicapt_BR
dc.contributor.butantanDisner, Geonildo Rodrigo|:Desvinculado|:Lab. de Toxinologia Aplicada:CeTICS - Centro de Toxinas, Resposta-imune e Sinalização Celularpt_BR
dc.contributor.butantanLima, Carla|:Pesquisador:Docente permanente PPGTox|:Lab. de Toxinologia Aplicada:CeTICS - Centro de Toxinas, Resposta-imune e Sinalização Celularpt_BR
dc.sponsorship.butantan(FAPESP) Fundação de Amparo à Pesquisa do Estado de São Paulo¦¦2013/07467-1pt_BR
dc.sponsorship.butantan(CAPES) Coordenação de Aperfeiçoamento de Pessoal de Nível Superior¦¦001pt_BR
dc.sponsorship.butantan(CNPq) Conselho Nacional de Desenvolvimento Científico e Tecnológico¦¦305414/2019-4pt_BR
dc.identifier.bvsccBR78.1pt_BR
dc.identifier.bvsdbIBProdpt_BR
dc.description.dbindexedYespt_BR
item.languageiso639-1English-
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