Pain and cellular migration induced by Bothrops jararaca venom in mice selected for an acute inflammatory response: involvement of mast cells


External affiliation
Publication type
Article
Language
English
Access rights
Open access
Terms of use
CC BY
Appears in Collections:
Metrics
Abstract
Bothrops jararaca venom (BjV) can induce mast cell degranulation. In order to investigate the role of mast cells and the interference of the host genetic background in the inflammation induced by BjV, we have used mouse strains selected for maximal (AIRmax) or minimal (AIRmin) acute inflammatory response (AIR). Mice were pretreated with an inhibitor of mast cell degranulation, cromolyn (CROM), and injected in footpads or intraperitoneally (i.p.) with BjV. Pain was measured with von Frey hairs, cell migration in the peritoneum by flow cytometry, and reactive oxygen species (ROS) production by chemiluminescence assays. The nociceptive response to BjV was higher in AIRmax than AIRmin mice; however, this difference was abolished by pretreatment with CROM. BjV induced peritoneal neutrophil (CD11b+ GR-1+) infiltration and ROS secretion in AIRmax mice only, which were partially inhibited by CROM. Our findings evidence a role for mast cells in pain, neutrophil migration, and ROS production triggered by BjV in AIRmax mice that are more susceptible to the action of BjV.
Reference
Kondo FV, Cabrera WHK, Franco MD, Picolo G, Sant´Anna MBM, Spadafora-Ferreira M, et al. Pain and cellular migration induced by Bothrops jararaca venom in mice selected for an acute inflammatory response: involvement of mast cells. Front. Immunol. 2022 Feb;12:779473. doi:10.3389/fimmu.2021.779473.
Link to cite this reference
https://repositorio.butantan.gov.br/handle/butantan/4150
URL
https://doi.org/10.3389/fimmu.2021.779473
Issue Date
2022


Files in This Item:

fimmu-12-779473.pdf
Description:
Size: 3.08 MB
Format: Adobe PDF
View/Open
Show full item record

This item is licensed under a Creative Commons License Creative Commons