Detection of SARS-CoV-2 virus via dynamic light scattering using antibody-gold nanoparticle bioconjugates against viral spike protein

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dc.contributorCentro Bioindustrialpt_BR
dc.contributor(LDI) Laboratório de Desenvolvimento e Inovação Industrialpt_BR
dc.contributor.authorSilva, Patricia Bento dapt_BR
dc.contributor.authorSilva, Jaqueline Rodrigues dapt_BR
dc.contributor.authorRodrigues, Mosar Corrêapt_BR
dc.contributor.authorVieira, Julia Augustopt_BR
dc.contributor.authorAndrade, Ikaro Alves dept_BR
dc.contributor.authorNagata, Tatsuyapt_BR
dc.contributor.authorSantos, Alexandre Silvapt_BR
dc.contributor.authorSilva, Sebastião William dapt_BR
dc.contributor.authorRocha, Marcia Cristina Oliveira dapt_BR
dc.contributor.authorBáo, Sônia Nairpt_BR
dc.contributor.authorMoraes-Vieira, Pedro M.pt_BR
dc.contributor.authorProença-Modena, Josépt_BR
dc.contributor.authorAngelim, Monara K.C.pt_BR
dc.contributor.authorSouza, Gabriela Fabiano dept_BR
dc.contributor.authorMuraro, Stefanie Primonpt_BR
dc.contributor.authorBarros, André Luis Branco dept_BR
dc.contributor.authorMartins, Glêndara Aparecida de Souzapt_BR
dc.contributor.authorRibeiro-Dias, Fátimapt_BR
dc.contributor.authorMachado, Giovannapt_BR
dc.contributor.authorFessel, Melissa Reginapt_BR
dc.contributor.authorChudzinski-Tavassi, Ana Marisapt_BR
dc.contributor.authorRonconi, Célia Machadopt_BR
dc.contributor.authorGonçalves, Deborapt_BR
dc.contributor.authorCuri, Ruipt_BR
dc.contributor.authorOliveira, Osvaldo N.pt_BR
dc.contributor.authorAzevedo, Ricardo Bentespt_BR
dc.date.accessioned2022-03-21T19:12:09Z-
dc.date.available2022-03-21T19:12:09Z-
dc.date.issued2022pt_BR
dc.identifier.citationSilva PB, Silva JR, Rodrigues MC, Vieira JA, Andrade IA, Nagata T, et al. Detection of SARS-CoV-2 virus via dynamic light scattering using antibody-gold nanoparticle bioconjugates against viral spike protein. Talanta. 2022 June;243:123355. doi:10.1016/j.talanta.2022.123355.pt_BR
dc.identifier.urihttps://repositorio.butantan.gov.br/handle/butantan/4266-
dc.description.abstractMass testing for the diagnosis of COVID-19 has been hampered in many countries owing to the high cost of genetic material detection. This study reports on a low-cost immunoassay for detecting SARS-CoV-2 within 30 min using dynamic light scattering (DLS). The immunosensor comprises 50-nm gold nanoparticles (AuNPs) functionalized with antibodies against SARS-CoV-2 spike glycoprotein, whose bioconjugation was confirmed using transmission electron microscopy (TEM), UV-Vis spectroscopy, Fourier transform infrared spectroscopy (FTIR), and surface-enhanced Raman scattering spectroscopy (SERS). The specific binding of the bioconjugates to the spike protein led to an increase in bioconjugate size, with a limit of detection (LOD) 5.29 × 103 TCID50/mL (Tissue Culture Infectious Dose). The immunosensor was also proven to be selective upon interaction with influenza viruses once no increase in size was observed after DLS measurement. The strategy proposed here aimed to use antibodies conjugated to AuNPs as a generic platform that can be extended to other detection principles, enabling technologies for low-cost mass testing for COVID-19.pt_BR
dc.description.sponsorship(CNPq) Conselho Nacional de Desenvolvimento Científico e Tecnológicopt_BR
dc.description.sponsorship(FAPERJ) Fundação de Amparo à Pesquisa do Estado do Rio de Janeiropt_BR
dc.description.sponsorship(CAPES) Coordenação de Aperfeiçoamento de Pessoal de Nível Superiorpt_BR
dc.description.sponsorship(FAPESP) Fundação de Amparo à Pesquisa do Estado de São Paulopt_BR
dc.format.extent123355pt_BR
dc.language.isoEnglishpt_BR
dc.relation.ispartofTalantapt_BR
dc.rightsOpen accesspt_BR
dc.rights.urihttps://creativecommons.org/licenses/by/4.0/pt_BR
dc.titleDetection of SARS-CoV-2 virus via dynamic light scattering using antibody-gold nanoparticle bioconjugates against viral spike proteinpt_BR
dc.typeArticlept_BR
dc.rights.licenseCC BYpt_BR
dc.identifier.doi10.1016/j.talanta.2022.123355pt_BR
dc.identifier.urlhttps://doi.org/10.1016/j.talanta.2022.123355pt_BR
dc.contributor.external(UNB) Universidade de Brasíliapt_BR
dc.contributor.external(UNICAMP) Universidade Estadual de Campinaspt_BR
dc.contributor.external(UFMG) Universidade Federal de Minas Geraispt_BR
dc.contributor.external(UFT) Universidade Federal do Tocantinspt_BR
dc.contributor.external(UFG) Universidade Federal de Goiáspt_BR
dc.contributor.external(CETENE) Centro de Tecnologias Estratégicas do Nordestept_BR
dc.contributor.external(UFF) Universidade Federal Fluminensept_BR
dc.contributor.external(USP) Universidade de São Paulopt_BR
dc.contributor.external(UNICSUL) Universidade Cruzeiro do Sulpt_BR
dc.identifier.citationvolume243pt_BR
dc.subject.keywordSARS-CoV-2pt_BR
dc.subject.keywordImmunosensorspt_BR
dc.subject.keywordBioconjugatespt_BR
dc.subject.keywordSpectroscopic techniquespt_BR
dc.subject.keywordInfluenza viruspt_BR
dc.relation.ispartofabbreviatedTalantapt_BR
dc.identifier.citationabntv. 243, 123355, jun. 2022pt_BR
dc.identifier.citationvancouver2022 June;243:123355pt_BR
dc.contributor.butantanFessel, Melissa Regina|:Técnico|:Centro Bioindustrialpt_BR
dc.contributor.butantanChudzinski-Tavassi, Ana Marisa|:Pesquisador:Docente Permanente PPGTox|:Lab. Desenvolvimento e Inovação Industrialpt_BR
dc.contributor.butantanCuri, Rui|:Diretor|:Centro Bioindustrialpt_BR
dc.sponsorship.butantan(CNPq) Conselho Nacional de Desenvolvimento Científico e Tecnológico¦¦402816/2020-0pt_BR
dc.sponsorship.butantan(FAPERJ) Fundação de Amparo à Pesquisa do Estado do Rio de Janeiro¦¦E26/210.194/2020pt_BR
dc.sponsorship.butantan(CAPES) Coordenação de Aperfeiçoamento de Pessoal de Nível Superior¦¦pt_BR
dc.sponsorship.butantan(FAPESP) Fundação de Amparo à Pesquisa do Estado de São Paulo¦¦2018/22214-6pt_BR
dc.identifier.bvsccBR78.1pt_BR
dc.identifier.bvsdbIBProdpt_BR
dc.description.dbindexedYespt_BR
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