The phytoactive constituents of Eugenia selloi B.D. Jacks (pitangatuba): toxicity and elucidation of their anti-inflammatory mechanism(s) of action


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Article
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English
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Open access
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Abstract
We determined the phytochemical composition, anti-inflammatory mechanism of action, ROS/RNS scavenging capacity and systemic toxicity of a purified subfraction (S8) of Eugenia selloi. The composition of S8 was assessed by LC-ESI-QTOF-MS; the anti-inflammatory activity in RAW264.7 macrophages through NF-κB activation and biomarkers by multiplex in THP-1 cells; neutrophil migration, intravital microscopy and ICAM-1 expression in mice; NETs formation and CD11b expression; S8 scavenging capacity of ROS/RNS; toxicity in Galleria mellonella larvae model. Coumaric acid, quercetrin and vanillic acid were identified. S8 decreased NF-κB activation, IL-1β, IL-6, IL-10, MDC and MCP-1 levels, reduced neutrophil migration and ICAM-1 expression in mice; S8 did not interfere NET formation and CD11b expression, exhibited high antioxidant and showed negligible toxicity. E. selloi proved to be a promising, yet underexplored source of bioactive compounds, which can be useful employed in agribusiness and in the pharmaceutical and food industry to develop new products or human health supplies.
Reference
Lazarini JG, Massarioli AP, Soares JC, Nani BD, Charo N, Oliveira DS, et al. The phytoactive constituents of Eugenia selloi B.D. Jacks (pitangatuba): toxicity and elucidation of their anti-inflammatory mechanism(s) of action. Food Chem. 2022 July;4:100093. doi:10.1016/j.fochms.2022.100093.
Link to cite this reference
https://repositorio.butantan.gov.br/handle/butantan/4268
Issue Date
2022


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