Proteomic profiling of hydatid fluid from pulmonary cystic echinococcosis

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dc.contributorLab. de Toxinologia Aplicadapt_BR
dc.contributorCeTICS - Centro de Toxinas, Resposta-imune e Sinalização Celularpt_BR
dc.contributor.authorSantos, Guilherme Brzoskowski dospt_BR
dc.contributor.authorSilva, Edileuza Danieli dapt_BR
dc.contributor.authorKitano, Eduardo Shigueopt_BR
dc.contributor.authorBattistella, Maria Eduardapt_BR
dc.contributor.authorMonteiro, Karina Mariantept_BR
dc.contributor.authorLima, Jeferson Camargo dept_BR
dc.contributor.authorFerreira, Henrique Bunselmeyerpt_BR
dc.contributor.authorSerrano, Solange Maria de Toledopt_BR
dc.contributor.authorZaha, Arnaldopt_BR
dc.date.accessioned2022-03-23T15:58:47Z-
dc.date.available2022-03-23T15:58:47Z-
dc.date.issued2022pt_BR
dc.identifier.citationSantos GB, Silva ED, Kitano ES, Battistella ME, Monteiro KM, Lima JC, et al. Proteomic profiling of hydatid fluid from pulmonary cystic echinococcosis. Parasit. Vectors. 2022 Mar;15:99. doi:10.1186/s13071-022-05232-8.pt_BR
dc.identifier.urihttps://repositorio.butantan.gov.br/handle/butantan/4269-
dc.description.abstractBackground Most cystic echinococcosis cases in Southern Brazil are caused by Echinococcus granulosus and Echinococcus ortleppi. Proteomic studies of helminths have increased our knowledge about the molecular survival strategies that are used by parasites. Here, we surveyed the protein content of the hydatid fluid compartment in E. granulosus and E. ortleppi pulmonary bovine cysts to better describe and compare their molecular arsenal at the host-parasite interface. Methods Hydatid fluid samples from three isolates of each species were analyzed using mass spectrometry-based proteomics (LC-MS/MS). In silico functional analyses of the identified proteins were performed to examine parasite survival strategies. Results The identified hydatid fluid protein profiles showed a predominance of parasite proteins compared to host proteins that infiltrate the cysts. We identified 280 parasitic proteins from E. granulosus and 251 from E. ortleppi, including 52 parasitic proteins that were common to all hydatid fluid samples. The in silico functional analysis revealed important molecular functions and processes that are active in pulmonary cystic echinococcosis, such as adhesion, extracellular structures organization, development regulation, signaling transduction, and enzyme activity. Conclusions The protein profiles described here provide evidence of important mechanisms related to basic cellular processes and functions that act at the host-parasite interface in cystic echinococcosis. The molecular tools used by E. granulosus and E. ortleppi for survival within the host are potential targets for new therapeutic approaches to treat cystic echinococcosis and other larval cestodiases.pt_BR
dc.description.sponsorship(CNPq) Conselho Nacional de Desenvolvimento Científico e Tecnológicopt_BR
dc.description.sponsorship(FAPERGS) Fundação de Amparo à pesquisa do Estado do Rio Grande do Sulpt_BR
dc.description.sponsorship(FAPESP) Fundação de Amparo à Pesquisa do Estado de São Paulopt_BR
dc.description.sponsorship(CAPES) Coordenação de Aperfeiçoamento de Pessoal de Nível Superiorpt_BR
dc.format.extent99pt_BR
dc.language.isoEnglishpt_BR
dc.relation.ispartofParasites & Vectorspt_BR
dc.rightsOpen accesspt_BR
dc.rights.urihttps://creativecommons.org/licenses/by/4.0/pt_BR
dc.titleProteomic profiling of hydatid fluid from pulmonary cystic echinococcosispt_BR
dc.typeArticlept_BR
dc.rights.licenseCC BYpt_BR
dc.identifier.doi10.1186/s13071-022-05232-8pt_BR
dc.identifier.urlhttps://doi.org/10.1186/s13071-022-05232-8pt_BR
dc.contributor.external(UFRGS) Universidade Federal do Rio Grande do Sulpt_BR
dc.identifier.citationvolume15pt_BR
dc.subject.keywordEchinococcus granulosuspt_BR
dc.subject.keywordEchinococcus ortleppipt_BR
dc.subject.keywordsecretomept_BR
dc.subject.keywordHydatid fluidpt_BR
dc.subject.keywordParasite proteomicspt_BR
dc.subject.keywordHost-parasite interfacept_BR
dc.relation.ispartofabbreviatedParasit. Vectorspt_BR
dc.identifier.citationabntv. 15, 99, mar. 2022pt_BR
dc.identifier.citationvancouver2022 Mar;15:99pt_BR
dc.contributor.butantanKitano, Eduardo Shigueo|:Desvinculado|:Lab. de Toxinologia Aplicada:CeTICS - Centro de Toxinas, Resposta-imune e Sinalização Celularpt_BR
dc.contributor.butantanSerrano, Solange Maria de Toledo|:Pesquisador:Docente Permanente PPGTox|:Lab. de Toxinologia Aplicada:CeTICS - Centro de Toxinas, Resposta-imune e Sinalização Celularpt_BR
dc.sponsorship.butantan(CNPq) Conselho Nacional de Desenvolvimento Científico e Tecnológico¦¦472316/2013-3pt_BR
dc.sponsorship.butantan(CNPq) Conselho Nacional de Desenvolvimento Científico e Tecnológico¦¦470716/2014-2pt_BR
dc.sponsorship.butantan(FAPERGS) Fundação de Amparo à pesquisa do Estado do Rio Grande do Sul¦¦001892-25.51/13-0pt_BR
dc.sponsorship.butantan(FAPESP) Fundação de Amparo à Pesquisa do Estado de São Paulo¦¦2013/07467-1pt_BR
dc.sponsorship.butantan(CAPES) Coordenação de Aperfeiçoamento de Pessoal de Nível Superior¦¦PARASITOLOGIA-1278/2011pt_BR
dc.sponsorship.butantan(CAPES) Coordenação de Aperfeiçoamento de Pessoal de Nível Superior¦¦23038.010043/2013-02pt_BR
dc.identifier.bvsccBR78.1pt_BR
dc.identifier.bvsdbIBProdpt_BR
dc.description.dbindexedYespt_BR
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