Role of AFP in adherence and colonization of intestinal and urinary tracts

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Translated title
Papel de AFP na aderência e colonização dos tratos intestinal e urinário

Responsible researcher
Butantan affiliation
Publication type
Dataset
Language
English
File format
pdf
Updated on
4-May-2022
Access rights
Open access
Appears in Collections:
Dataset description
Quantitative adherence and invasion of UPEC-46 (.pdf): quantitative adherence and invasion assays (3 hours of incubation, without D-mannose) of UPEC-46 using different eukaryotic cell lineages. Interaction and invasion ability of UPEC-46 and controls in different cells (.pdf): total interaction, internalized bacteria and invasion of UPEC-46 and control strains (3 hours of incubation, without D-mannose) using different eukaryotic cell lineages. Effect of D-mannose in adherence of UPEC-46 (.pdf): effect of D-mannose on T1P-mediated adherence of UPEC-46 strain in different eukaryotic cell lineages. Quantitative adherence of UPEC-46 and derivate strains in different cells (.pdf): quantitative adherence assays (3 hours of incubation, without D-mannose) of UPEC-46 and derivate strains using different eukaryotic cell lineages. Bladders and kidneys colonization UPEC-46 (.pdf): Bladders and kidneys colonization mediated by UPEC-46 strain in UTI mouse model (6, 12, 24, or 72 h). Urinary colonization UPEC-46 and derivate strains (.pdf): Bladders and kidneys colonization mediated by UPEC-46 and derivate strains in UTI mouse model (72 h). Intestinal colonization UPEC-46 and derivate strains (.pdf): Mice were orogastrically inoculated with 103 CFU of UPEC-46 and derivate strains. Fresh fecal samples were collected from each mouse for up to 14 d post-inoculation for bacteria counts. Area under curve UPEC-46 and derivate strains (.pdf): cumulative bacterial enumeration for 14 d post infection [CFU/g feces]. Growth curve of UPEC-46 and double mutant (.pdf): growth curve of UPEC-46 and double mutant in different culture mediums. Motility of UPEC-46 and double mutant (.pdf): halo diameter (cm) of UPEC-46 and double mutant on LB agar containing 0.3% agar. Steps of fimH mutagenesis (.pdf): steps employed to construct the fimH mutant strain using UPEC-46::afpA – primers and PCR amplicons analyzed by agarose gels. Blast 204 mutant primer Fw (.pdf): BLAST from Sanger sequencing to the forward primer (KAN-2 FP-1) of 204 transposon mutant using UPEC-46 strain as query sequence. Blast 204 mutant primer Rv (.pdf): BLAST from Sanger sequencing to the reverse primer (R6KAN-2 RP-1) of 204 transposon mutant using UPEC-46 strain as query sequence. Blast 310 mutant primer Fw (.pdf): BLAST from Sanger sequencing to the forward primer (KAN-2 FP-1) of 310 transposon mutant using UPEC-46 strain as query sequence. Blast 310 mutant primer Rv (.pdf): BLAST from Sanger sequencing to the reverse primer (R6KAN-2 RP-1) of 310 transposon mutant using UPEC-46 strain as query sequence. Blast 316 mutant primer Fw (.pdf): BLAST from Sanger sequencing to the forward primer (KAN-2 FP-1) of 316 transposon mutant using UPEC-46 strain as query sequence. Blast 316 mutant primer Rv (.pdf): BLAST from Sanger sequencing to the reverse primer (R6KAN-2 RP-1) of 316 transposon mutant using UPEC-46 strain as query sequence. Blast 441 mutant primer Fw (.pdf): BLAST from Sanger sequencing to the forward primer (KAN-2 FP-1) of 441 transposon mutant using UPEC-46 strain as query sequence. Blast 441 mutant primer Rv (.pdf): BLAST from Sanger sequencing to the reverse primer (R6KAN-2 RP-1) of 441 transposon mutant using UPEC-46 strain as query sequence. Blast 632 mutant primer Fw (.pdf): BLAST from Sanger sequencing to the forward primer (KAN-2 FP-1) of 632 transposon mutant using UPEC-46 strain as query sequence. Blast 632 mutant primer Rv (.pdf): BLAST from Sanger sequencing to the reverse primer (R6KAN-2 RP-1) of 632 transposon mutant using UPEC-46 strain as query sequence. Blast 700 mutant primer Fw (.pdf): BLAST from Sanger sequencing to the forward primer (KAN-2 FP-1) of 700 transposon mutant using UPEC-46 strain as query sequence. Blast 700 mutant primer Rv (.pdf): BLAST from Sanger sequencing to the reverse primer (R6KAN-2 RP-1) of 700 transposon mutant using UPEC-46 strain as query sequence. Blast 780 mutant primer Fw (.pdf): BLAST from Sanger sequencing to the forward primer (KAN-2 FP-1) of 780 transposon mutant using UPEC-46 strain as query sequence. Blast 780 mutant primer Rv (.pdf): BLAST from Sanger sequencing to the reverse primer (R6KAN-2 RP-1) of 780 transposon mutant using UPEC-46 strain as query sequence. Blast 781 mutant primer Fw (.pdf): BLAST from Sanger sequencing to the forward primer (KAN-2 FP-1) of 781 transposon mutant using UPEC-46 strain as query sequence. Blast 781 mutant primer Rv (.pdf): BLAST from Sanger sequencing to the reverse primer (R6KAN-2 RP-1) of 781 transposon mutant using UPEC-46 strain as query sequence. Blast 854 mutant primer Fw (.pdf): BLAST from Sanger sequencing to the forward primer (KAN-2 FP-1) of 854 transposon mutant using UPEC-46 strain as query sequence. Blast 854 mutant primer Rv (.pdf): BLAST from Sanger sequencing to the reverse primer (R6KAN-2 RP-1) of 854 transposon mutant using UPEC-46 strain as query sequence. Blast 901 mutant primer Fw (.pdf): BLAST from Sanger sequencing to the forward primer (KAN-2 FP-1) of 901 transposon mutant using UPEC-46 strain as query sequence. Blast 901 mutant primer Rv (.pdf): BLAST from Sanger sequencing to the reverse primer (R6KAN-2 RP-1) of 901 transposon mutant using UPEC-46 strain as query sequence. Blast 936 mutant primer Fw (.pdf): BLAST from Sanger sequencing to the forward primer (KAN-2 FP-1) of 936 transposon mutant using UPEC-46 strain as query sequence. Blast 936 mutant primer Rv (.pdf): BLAST from Sanger sequencing to the reverse primer (R6KAN-2 RP-1) of 936 transposon mutant using UPEC-46 strain as query sequence. Blast 976 mutant primer Fw (.pdf): BLAST from Sanger sequencing to the forward primer (KAN-2 FP-1) of 976 transposon mutant using UPEC-46 strain as query sequence. Blast 976 mutant primer Rv (.pdf): BLAST from Sanger sequencing to the reverse primer (R6KAN-2 RP-1) of 976 transposon mutant using UPEC-46 strain as query sequence. Intestinal colonization model– PCR (.pdf): Mice were orogastrically inoculated with 103 CFU of UPEC-46 and derivate strains. Fresh fecal samples were collected from each mouse for up to 14 d post-inoculation for bacteria counts, using MacConkey agar. PCRs were performed using the primers for afpA and afpP genes amplification. UTI model– PCR (.pdf): Mice were transurethrally inoculated with 109 CFU of UPEC-46 and derivative strains. After inoculation, mice were sacrificed and the bladders and kidneys were collected for bacterial counts, using MacConkey agar. PCRs were performed using the primers for afpA and afpP genes amplification.
Link to cite this reference
https://repositorio.butantan.gov.br/handle/butantan/4315
Issue Date
2022


Files in This Item:

Area under curve UPEC-46 and derivate strains.pdf
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Size: 12.76 kB
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Bladders and kidneys colonization UPEC-46.pdf
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Size: 70.34 kB
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Blast 204 mutant primer Fw.pdf
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Size: 201.42 kB
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Blast 204 mutant primer Rv.pdf
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Size: 197.88 kB
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Blast 310 mutant primer Fw.pdf
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Size: 196.18 kB
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Blast 310 mutant primer Rv.pdf
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Size: 196.82 kB
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Blast 316 mutant primer Fw.pdf
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Size: 198.68 kB
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Blast 316 mutant primer Rv.pdf
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Size: 200.54 kB
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Blast 441 mutant primer Fw.pdf
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Size: 421.88 kB
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Blast 441 mutant primer Rv.pdf
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Size: 199.09 kB
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Blast 632 mutant primer Fw.pdf
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Size: 199.18 kB
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Blast 632 mutant primer Rv.pdf
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Size: 199.16 kB
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Blast 700 mutant primer Fw.pdf
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Size: 198.29 kB
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Blast 700 mutant primer Rv.pdf
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Size: 195.74 kB
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Blast 780 mutant primer Fw.pdf
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Size: 196.01 kB
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Blast 780 mutant primer Rv.pdf
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Size: 196.86 kB
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Blast 781 mutant primer Fw.pdf
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Size: 284.61 kB
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Blast 781 mutant primer Rv.pdf
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Size: 285.86 kB
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Blast 854 mutant primer Fw.pdf
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Size: 282.65 kB
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Blast 854 mutant primer Rv.pdf
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Size: 287.41 kB
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Blast 901 mutant primer Fw.pdf
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Size: 280.45 kB
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Blast 901 mutant primer Rv.pdf
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Size: 283.21 kB
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Blast 936 mutant primer Fw.pdf
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Size: 285.1 kB
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Blast 936 mutant primer Rv.pdf
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Size: 285.76 kB
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Blast 976 mutant primer Fw.pdf
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Size: 277.98 kB
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Blast 976 mutant primer Rv.pdf
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Size: 276.48 kB
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Effect of D-mannose in adherence of UPEC-46.pdf
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Size: 19.61 kB
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Growth curve of UPEC-46 and double mutant.pdf
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Size: 36.28 kB
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Intestinal colonization model_ PCR.pdf
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Size: 868.94 kB
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Intestinal colonization UPEC-46 and derivate strains.pdf
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Size: 107.08 kB
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Motility of UPEC-46 and double mutant.pdf
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Size: 10.36 kB
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Quantitative adherence and invasion of UPEC-46.pdf
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Size: 16.48 kB
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Steps of fimH mutagenesis.pdf
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Size: 339.53 kB
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Urinary colonization UPEC-46 and derivate strains.pdf
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Size: 36.16 kB
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UTI model_ PCR.pdf
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Size: 250.31 kB
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