Recapitulation of retinal damage in zebrafish larvae infected with Zika virus

Full metadata record
DC FieldValueLanguage
dc.contributorLab. de Toxinologia Aplicadapt_BR
dc.contributor(LMP) Laboratório Multipropósitopt_BR
dc.contributor.authorMaleski, Adolfo Luis Almeidapt_BR
dc.contributor.authorRosa, João Gabriel Santospt_BR
dc.contributor.authorBernardo, Jefferson Thiago Gonçalvespt_BR
dc.contributor.authorAstray, Renato Mancinipt_BR
dc.contributor.authorWalker, Cristiani Isabel Banderópt_BR
dc.contributor.authorLopes-Ferreira, Monicapt_BR
dc.contributor.authorLima, Carlapt_BR
dc.date.accessioned2022-05-06T18:58:35Z-
dc.date.available2022-05-06T18:58:35Z-
dc.date.issued2022pt_BR
dc.identifier.citationMaleski ALA, Rosa JGS, Bernardo JTG, Astray RM, Walker CIB, Lopes-Ferreira M, et al. Recapitulation of retinal damage in zebrafish larvae infected with Zika virus. Cells. 2022; Apr 11(9):157. doi:10.3390/cells11091457.pt_BR
dc.identifier.urihttps://repositorio.butantan.gov.br/handle/butantan/4316-
dc.description.abstractZebrafish are increasingly being utilized as a model to investigate infectious diseases and to advance the understanding of pathogen–host interactions. Here, we take advantage of the zebrafish to recapitulate congenital ZIKV infection and, for the first time, demonstrate that it can be used to model infection and reinfection and monitor anti-viral and inflammatory immune responses, as well as brain growth and eye abnormalities during embryonic development. By injecting a Brazilian strain of ZIKV into the yolk sac of one-cell stage embryos, we confirmed that, after 72 h, ZIKV successfully infected larvae, and the physical condition of the virus-infected hosts included gross morphological changes in surviving embryos (84%), with a reduction in larval head size and retinal damage characterized by increased thickness of the lens and inner nuclear layer. Changes in locomotor activity and the inability to perceive visual stimuli are a result of changes in retinal morphology caused by ZIKV. Furthermore, we demonstrated the ability of ZIKV to replicate in zebrafish larvae and infect new healthy larvae, impairing their visual and neurological functions. These data reinforce the deleterious activity of ZIKV in the brain and visual structures and establish the zebrafish as a model to study the molecular mechanisms involved in the pathology of the virus.pt_BR
dc.description.sponsorship(FAPESP) Fundação de Amparo à Pesquisa do Estado de São Paulopt_BR
dc.description.sponsorship(CNPq) Conselho Nacional de Desenvolvimento Científico e Tecnológicopt_BR
dc.description.sponsorship(CAPES) Coordenação de Aperfeiçoamento de Pessoal de Nível Superiorpt_BR
dc.format.extent1457pt_BR
dc.language.isoEnglishpt_BR
dc.relation.ispartofCellspt_BR
dc.rightsOpen accesspt_BR
dc.rights.urihttps://creativecommons.org/licenses/by/4.0/pt_BR
dc.titleRecapitulation of retinal damage in zebrafish larvae infected with Zika viruspt_BR
dc.typeArticlept_BR
dc.rights.licenseCC BYpt_BR
dc.identifier.doi10.3390/cells11091457pt_BR
dc.identifier.urlhttps://doi.org/10.3390/cells11091457pt_BR
dc.contributor.external(UFS) Universidade Federal de Sergipept_BR
dc.identifier.citationvolume11pt_BR
dc.identifier.citationissue9pt_BR
dc.subject.keywordZika viruspt_BR
dc.subject.keywordzebrafishpt_BR
dc.subject.keyworddevelopmentpt_BR
dc.subject.keywordretinopathypt_BR
dc.subject.keywordlocomotor behaviorpt_BR
dc.relation.ispartofabbreviatedCellspt_BR
dc.identifier.citationabntv. 11, n. 9, p. 157, abr. 2022pt_BR
dc.identifier.citationvancouver2022; Apr 11(9):157pt_BR
dc.contributor.butantanMaleski, Adolfo Luis Almeida|:Aluno|:Lab. de Toxinologia Aplicadapt_BR
dc.contributor.butantanRosa, João Gabriel Santos|:Aluno|:Lab. de Toxinologia Aplicadapt_BR
dc.contributor.butantanBernardo, Jefferson Thiago Gonçalves|:Aluno|:Lab. de Toxinologia Aplicadapt_BR
dc.contributor.butantanAstray, Renato Mancini|:Pesquisador|:(LMP) Laboratório Multipropósitopt_BR
dc.contributor.butantanLopes-Ferreira, Monica|:Pesquisador|:Lab. de Toxinologia Aplicadapt_BR
dc.contributor.butantanLima, Carla|:Pesquisador|:Lab. de Toxinologia Aplicadapt_BR
dc.sponsorship.butantan(FAPESP) Fundação de Amparo à Pesquisa do Estado de São Paulo¦¦2013/07467-1pt_BR
dc.sponsorship.butantan(CNPq) Conselho Nacional de Desenvolvimento Científico e Tecnológico¦¦305414/2019-4pt_BR
dc.sponsorship.butantan(CAPES) Coordenação de Aperfeiçoamento de Pessoal de Nível Superior¦¦001pt_BR
dc.identifier.bvsccBR78.1pt_BR
dc.identifier.bvsdbIBProdpt_BR
dc.description.dbindexedYespt_BR
item.grantfulltextopen-
item.languageiso639-1English-
item.fulltextCom Texto completo-
item.openairetypeArticle-
crisitem.author.dept#PLACEHOLDER_PARENT_METADATA_VALUE#-
crisitem.author.dept#PLACEHOLDER_PARENT_METADATA_VALUE#-
crisitem.author.dept#PLACEHOLDER_PARENT_METADATA_VALUE#-
crisitem.author.dept#PLACEHOLDER_PARENT_METADATA_VALUE#-
crisitem.author.dept#PLACEHOLDER_PARENT_METADATA_VALUE#-
crisitem.author.dept#PLACEHOLDER_PARENT_METADATA_VALUE#-
crisitem.author.dept#PLACEHOLDER_PARENT_METADATA_VALUE#-
crisitem.author.orcid#PLACEHOLDER_PARENT_METADATA_VALUE#-
crisitem.author.orcid#PLACEHOLDER_PARENT_METADATA_VALUE#-
crisitem.author.orcid#PLACEHOLDER_PARENT_METADATA_VALUE#-
crisitem.author.orcid0000-0002-6983-5298-
crisitem.author.orcid#PLACEHOLDER_PARENT_METADATA_VALUE#-
crisitem.author.orcid0000-0003-0244-7482-
crisitem.author.orcid0000-0002-8161-966X-
crisitem.author.parentorg#PLACEHOLDER_PARENT_METADATA_VALUE#-
crisitem.author.parentorg#PLACEHOLDER_PARENT_METADATA_VALUE#-
crisitem.author.parentorg#PLACEHOLDER_PARENT_METADATA_VALUE#-
crisitem.journal.journalissn#PLACEHOLDER_PARENT_METADATA_VALUE#-
crisitem.journal.journaleissn#PLACEHOLDER_PARENT_METADATA_VALUE#-
Appears in Collections:Artigos de periódicos


Files in This Item:

cells-11-01457.pdf
Description:
Size: 1.53 MB
Format: Adobe PDF
View/Open
Show simple item record

This item is licensed under a Creative Commons License Creative Commons