Macrophage inflammatory response mediated by intimin and bundle-Forming pilus from Enteropathogenic Escherichia coli

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dc.contributorLab. Bacteriologiapt_BR
dc.contributor.authorCaetano, Bruna Alvespt_BR
dc.contributor.authorMourão, Daniela B.pt_BR
dc.contributor.authorAbreu, Patricia Antonia Estimapt_BR
dc.contributor.authorMonaris, Denizept_BR
dc.contributor.authorVasconcellos, Halyka L.pt_BR
dc.contributor.authorLuz, Danielapt_BR
dc.contributor.authorGalhardo, Cynthia Soarespt_BR
dc.contributor.authorMenezes, Marcio A.pt_BR
dc.contributor.authorLima, Flávia A.pt_BR
dc.contributor.authorElias, Waldir Pereirapt_BR
dc.contributor.authorBorges, Monamaris Marquespt_BR
dc.contributor.authorPiazza, Roxane Maria Fontespt_BR
dc.identifier.citationCaetano BA, Mourão DB., Abreu PAE, Monaris D, Vasconcellos HL., Luz D, et al. Macrophage inflammatory response mediated by intimin and bundle-Forming pilus from Enteropathogenic Escherichia coli. Proceedings. . doi:10.3390/proceedings2022081146.pt_BR
dc.description.abstractEnteropathogenic Escherichia coli (EPEC) are important agents of acute diarrhea in children living in developing countries. A severe dysfunction of the intestinal epithelial barrier occurs during EPEC infection, leading to diarrhea and inflammation as consequences. EPEC main virulence factors include the adhesins intimin and bundle-forming pilus (BFP), as well as several effector proteins translocated to the enterocyte by the type-three secretion system. The initial interaction of EPEC with the host cell and the role of effector proteins in this process are well known. However, the role of the EPEC virulence factors in macrophage activation is not fully understood. Hence, we analyzed the ability of intimin and bundle-forming pilus (BfpA) to activate the innate response mediated by macrophages, where the production of the proinflammatory cytokines TNF-α, IL-1, IL-6 and IL-12, as well as the anti-inflammatory cytokine IL-10 and chemokine MCP-1, were evaluated. Our results showed that recombinant intimin and BfpA activate macrophages in a dose-dependent manner, and the stimulated cells produced TNF-α, IL-12, IL-6, IL-10 and MCP-1, but not IL-1β. No synergistic effect was observed in the production of pro-inflammatory cytokines by combining BfpA and intimin, although production of IL-10, an anti-inflammatory mediator, was potentiated at a higher dose. The effect observed was largely attributed to these proteins, as the treatment of proteins with polymyxin B did not alter the production of TNF-α. Thus, herein we showed that intimin and BfpA can activate the innate immune response, inducing the production of pro- and anti-inflammatory cytokines, as well as chemokines, playing additional role as inflammatory molecules in the early steps of EPEC infection.pt_BR
dc.rightsOpen accesspt_BR
dc.titleMacrophage inflammatory response mediated by intimin and bundle-Forming pilus from Enteropathogenic Escherichia colipt_BR
dc.rights.licenseCC BYpt_BR
dc.contributor.externalFundação Pró-Sangue Hemocentro de São Paulopt_BR
dc.contributor.externalHospital Israelita Albert Einsteinpt_BR
dc.contributor.externalSuperintendência de Polícia Técnico Científicapt_BR
dc.subject.keywordenteropathogenic E. colipt_BR
dc.subject.keywordbundle-forming pilus (BfpA)pt_BR
dc.subject.keywordinnate immune responsept_BR
dc.contributor.butantanAbreu, Patricia Antonia Estima|:Pesquisador|:Lab. Bacteriologiapt_BR
dc.contributor.butantanCaetano, Bruna Alves|:Aluno|:Lab. Bacteriologiapt_BR
dc.contributor.butantanElias, Waldir Pereira|:Pesquisador|:Lab. Bacteriologiapt_BR
dc.contributor.butantanBorges, Monamaris Marques|:Pesquisador|:Lab. Bacteriologiapt_BR
dc.contributor.butantanPiazza, Roxane Maria Fontes|:Pesquisador|:Lab. Bacteriologiapt_BR
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