Biochemical and toxinological characterization of venom from Macrorhynchia philippina (Cnidaria, Hydrozoa)

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dc.contributorLab. Bioquímicapt_BR
dc.contributor.authorBanagouro, Karine Cristie Quagliopt_BR
dc.contributor.authorViana, Jeffersonpt_BR
dc.contributor.authorLima, Leonardo Pereira dept_BR
dc.contributor.authorCoelho, Guilherme Rabelopt_BR
dc.contributor.authorRocha, Thalitapt_BR
dc.contributor.authorGirardello, Raquelpt_BR
dc.contributor.authorRussi, Karolayne Larissapt_BR
dc.contributor.authorKitahara, Marcelo V.pt_BR
dc.contributor.authorSciani, Juliana Mozerpt_BR
dc.identifier.citationBanagouro KCQ, Viana J, Lima LP, Coelho GR, Rocha T, Girardello R, et al. Biochemical and toxinological characterization of venom from Macrorhynchia philippina (Cnidaria, Hydrozoa). Biomed Res Int. 2022 May; 2022:8170252. doi:10.1155/2022/8170252.pt_BR
dc.description.abstractMacrorhynchia philippina is a colonial benthic hydroid from the Class Hydrozoa (Phylum Cnidaria) distributed in the tropical and subtropical marine waters from Atlantic Ocean, Indo-Pacific, and Mozambique. Its colonies somewhat resemble plants, causing confusion in the bathers who accidentally touch the animal. Acute burning/local pain, edema, erythema, and pruritus were symptoms already described, but its venom composition is unknown, as well as the participation of toxins for the symptom’s development. Thus, herein, we show the biochemical composition and toxic effects of M. philippina venom. Colonies were collected and processed for histological analysis; alternatively, they were immersed into methanol containing 0.1% acetic acid for venom attainment, which was analyzed by mass spectrometry and submitted to edema and nociception evaluation in mice, hemolysis and antimicrobial assays in vitro. Before the molecule’s extraction, it was possible to see the inoculation structures (hydrocladiums and hydrotheca) containing venom, which was released after the immersion of the animal in the solvents. The venom was composed mainly by low molecular mass compounds, able to cause significant reduction of the paw withdrawal latency from the hot plate test, 30 minutes after the injection. Moreover, significant edema was observed 10 and 30 minutes after the injection, indicating the activity of at least two inflammatory mediators. The venom caused no hemolytic activity but reduced the growth of A. baumannii and K. pneumoniae strains. This study is the first biochemical description of M. philippina venom, with molecules that cause fast inflammatory and painful effects, characteristic of the envenomation.pt_BR
dc.description.sponsorship(FAPESP) Fundação de Amparo à Pesquisa do Estado de São Paulopt_BR
dc.relation.ispartofBioMed Research Internationalpt_BR
dc.rightsOpen accesspt_BR
dc.titleBiochemical and toxinological characterization of venom from Macrorhynchia philippina (Cnidaria, Hydrozoa)pt_BR
dc.rights.licenseCC BYpt_BR
dc.contributor.external(USF) Universidade São Franciscopt_BR
dc.contributor.external(PUC-SP) Pontifícia Universidade Católica de São Paulopt_BR
dc.contributor.external(USP) Universidade de São Paulopt_BR
dc.contributor.external(UNIFESP) Universidade Federal de São Paulopt_BR
dc.contributor.externalSmithsonian Institutionpt_BR
dc.relation.ispartofabbreviatedBiomed Res Intpt_BR
dc.identifier.citationabntv. 2022, 8170252, mai. 2022pt_BR
dc.identifier.citationvancouver2022 May; 2022:8170252pt_BR
dc.contributor.butantanCoelho, Guilherme Rabelo|:Técnico|:Lab. Bioquímicapt_BR
dc.sponsorship.butantan(FAPESP) Fundação de Amparo à Pesquisa do Estado de São Paulo¦¦2019/19929-6pt_BR
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