Influence of the Polysaccharide capsule on the bactericidal activity of indolicidin on Streptococcus pneumoniae

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dc.contributorLab. de Desenvolvimento de Vacinaspt_BR
dc.contributor.authorWaz, Natalha T.pt_BR
dc.contributor.authorOliveira, Sheilapt_BR
dc.contributor.authorGirardello, Raquelpt_BR
dc.contributor.authorLincopan, Niltonpt_BR
dc.contributor.authorBarazzone, Giovana Cappiopt_BR
dc.contributor.authorParisotto, Thaispt_BR
dc.contributor.authorHakansson, Anders P.pt_BR
dc.contributor.authorConverso, Thiago Rojaspt_BR
dc.contributor.authorDarrieux, Michellept_BR
dc.date.accessioned2022-06-03T12:04:53Z-
dc.date.available2022-06-03T12:04:53Z-
dc.date.issued2022pt_BR
dc.identifier.citationWaz NT., Oliveira S, Girardello R, Lincopan N, Barazzone GC, Parisotto T, et al. Influence of the Polysaccharide capsule on the bactericidal activity of indolicidin on Streptococcus pneumoniae. Front. Microbiol. 2022 May;13:898815. doi:10.3389/fmicb.2022.898815.pt_BR
dc.identifier.urihttps://repositorio.butantan.gov.br/handle/butantan/4380-
dc.description.abstractStreptococcus pneumoniae is a pathogen responsible for high morbidity and mortality worldwide. The polysaccharide capsule confers protection against phagocytosis and influences many aspects of pneumococcal pathogenesis. The capsular polysaccharides (CPS) are highly immunogenic and exhibit great structural variability, with more than 100 serotypes described so far. Antimicrobial peptides (AMPs) are an important part of the innate defense mechanisms against many pathogens. Indolicidin is a cationic AMP produced by bovine neutrophils, with bactericidal effects against several bacteria. CPS has been shown to interfere with the ability of AMPs to kill pneumococci, but the effects of capsule variability on susceptibility to indolicidin have not been explored. The present work determined the effects of capsule on resistance to indolicidin in vitro. Using a bactericidal plate assay, we observed that different pneumococcal serotypes exhibited variable resistance to indolicidin, which correlated with the capsule net charge. Interestingly, the effect of capsule expression on resistance to indolicidin was dependent on the serotype; bacteria with lower zeta potential were more resistant to indolicidin when capsule was present, while those with less negative surface charge were more resistant in the absence of capsule. The addition of purified CPS partially rescued the bacteria from the bactericidal effects of indolicidin, while the addition of anticapsular antibodies accentuated the peptide’s bactericidal action, suggesting a possible new protective mechanism induced by polysaccharide-based pneumococcal vaccines.pt_BR
dc.description.sponsorship(FAPESP) Fundação de Amparo à Pesquisa do Estado de São Paulopt_BR
dc.description.sponsorship(CAPES) Coordenação de Aperfeiçoamento de Pessoal de Nível Superiorpt_BR
dc.description.sponsorship(CNPq) Conselho Nacional de Desenvolvimento Científico e Tecnológicopt_BR
dc.description.sponsorship(SRC) Swedish Research Councilpt_BR
dc.format.extent898815pt_BR
dc.language.isoEnglishpt_BR
dc.relation.ispartofFrontiers in Microbiologypt_BR
dc.rightsOpen accesspt_BR
dc.rights.urihttps://creativecommons.org/licenses/by/4.0/pt_BR
dc.titleInfluence of the Polysaccharide capsule on the bactericidal activity of indolicidin on Streptococcus pneumoniaept_BR
dc.typeArticlept_BR
dc.rights.licenseCC BYpt_BR
dc.identifier.doi10.3389/fmicb.2022.898815pt_BR
dc.identifier.urlhttps://doi.org/10.3389/fmicb.2022.898815pt_BR
dc.contributor.external(USF) Universidade São Franciscopt_BR
dc.contributor.external(USP) Universidade de São Paulopt_BR
dc.contributor.externalLunds Universitetpt_BR
dc.identifier.citationvolume13pt_BR
dc.subject.keywordcapsular polysaccharidept_BR
dc.subject.keywordindolicidinpt_BR
dc.subject.keywordStreptococcus pneumoniaept_BR
dc.subject.keywordantimicrobial peptidespt_BR
dc.subject.keywordAMP resistancept_BR
dc.relation.ispartofabbreviatedFront. Microbiolpt_BR
dc.identifier.citationabntv. 13, 898815, maio. 2022pt_BR
dc.identifier.citationvancouver2022 May;13:898815pt_BR
dc.contributor.butantanBarazzone, Giovana Cappio|:Pesquisador|:Lab. de Desenvolvimento de Vacinaspt_BR
dc.sponsorship.butantan(FAPESP) Fundação de Amparo à Pesquisa do Estado de São Paulo¦¦2020/11037-6pt_BR
dc.sponsorship.butantan(CAPES) Coordenação de Aperfeiçoamento de Pessoal de Nível Superior¦¦88887.495999/2020–00pt_BR
dc.sponsorship.butantan(CNPq) Conselho Nacional de Desenvolvimento Científico e Tecnológico¦¦400099/2022-5pt_BR
dc.sponsorship.butantan(SRC) Swedish Research Council¦¦2021-06050pt_BR
dc.identifier.bvsccBR78.1pt_BR
dc.identifier.bvsdbIBProdpt_BR
dc.description.dbindexedYespt_BR
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