Multiplex PCR to Streptococcus pneumoniae serotype identification directly in cerebrospinal fluid samples

Full metadata record
DC FieldValueLanguage
dc.contributorLab. Bacteriologiapt_BR
dc.contributor.authorde Souza, Mariana Brenapt_BR
dc.contributor.authorCarvalho, Eneaspt_BR
dc.contributor.authorCergole-Novella, Maria Cecíliapt_BR
dc.contributor.authorMolinari, Delma Aparecidapt_BR
dc.contributor.authorColpas, Daniela Rodriguespt_BR
dc.contributor.authorCarmo, Andréia Moreira dos Santospt_BR
dc.contributor.authorDaros, Vilma dos Santos Menezes Gaiottopt_BR
dc.contributor.authorde Campos, Ivana Barrospt_BR
dc.identifier.citationde Souza MB, Carvalho E, Cergole-Novella MC, Molinari DA, Colpas DR, Carmo AMS, et al. Multiplex PCR to Streptococcus pneumoniae serotype identification directly in cerebrospinal fluid samples. Eur J Clin Microbiol Infect Dis. 2023 Jan; 42:255-266. doi:10.1007/s10096-023-04547-3.pt_BR
dc.description.abstractStreptococcus pneumoniae causes invasive diseases of significant public health concern, such as meningitis. The culture of cerebrospinal fluid (CSF) samples, the standard technique for meningitis diagnoses, is not always positive. Consequently, meaningful information about the etiological agent is lost, which can compromise effective epidemiological surveillance and the improvement of immunization policies. This study aims to standardize a method to genotype pneumococcus in the CSF samples which could mitigate the absence of isolated strains, and also evaluate the prediction of this assay. We applied eight multiplex PCR (mPCR) assays to CSF samples paired with the Quellung reaction applied to the isolated strains. We also compared different master mix kits in the mPCR. Moreover, a retrospective study was conducted with CSF samples considered pneumococcus positive due to the presence of the lytA gene. Results showed that genotyping by the mPCR correlated 100% with the Quellung reaction, and genotyping was dependent on the master mix applied. In the retrospective study (2014–2020), 73.4% were successfully genotyped. The analyses of the receiver operating characteristic curve showed that the cycle threshold (Ct value) around 30 for the lytA gene had a 75% positive chance of successful genotyping, whereas with a Ct value > 35, the chance was 12.5%. Finally, we observed that genotype 19A was prevalent in the period (12%), information unknown until now due to the lack of isolated strains. Therefore, the mPCR of CSF samples can efficiently predict S. pneumoniae serotypes, especially in the absence of isolated strains, which can be a great tool for pneumococcal serotype surveillance.pt_BR
dc.description.sponsorship(FAPESP) Fundação de Amparo à Pesquisa do Estado de São Paulopt_BR
dc.relation.ispartofEuropean Journal of Clinical Microbiology & Infectious Diseasespt_BR
dc.rightsRestricted accesspt_BR
dc.titleMultiplex PCR to Streptococcus pneumoniae serotype identification directly in cerebrospinal fluid samplespt_BR
dc.contributor.external(IAL) Instituto Adolfo Lutzpt_BR
dc.subject.keywordmolecular diagnosticpt_BR
dc.subject.keywordmultiplex PCRpt_BR
dc.subject.keywordpneumococcal meningitispt_BR
dc.subject.keywordpneumococcal serotypespt_BR
dc.subject.keywordcerebrospinal fluidpt_BR
dc.relation.ispartofabbreviatedEur J Clin Microbiol Infect Dispt_BR
dc.identifier.citationabntv. 42, 255-266, jan. 2023pt_BR
dc.identifier.citationvancouver2023 Jan; 42:255-266pt_BR
dc.contributor.butantanCarvalho, Eneas|:Pesquisador|:Lab. Bacteriologia|:pt_BR
dc.sponsorship.butantan(FAPESP) Fundação de Amparo à Pesquisa do Estado de São Paulo¦¦2018/22718–4pt_BR
dc.sponsorship.butantan(FAPESP) Fundação de Amparo à Pesquisa do Estado de São Paulo¦¦2017/03022–6pt_BR
dc.description.internalLiberado apenas a versão aceita c/ 12 meses de embargo sob Publisher's Bespoke Licensept_BR
item.fulltextSem Texto completo-
Appears in Collections:Artigos

Show simple item record

The access to the publications deposited in this repository respects the licenses from journals and publishers.