Analysis of LruC lipoprotein and identification of peptides candidates for vaccine development and diagnosis of leptospirosis

Full metadata record
DC FieldValueLanguage
dc.contributorLab. Bacteriologiapt_BR
dc.contributor(BCA) Lab. Biofármacospt_BR
dc.contributorCentro Bioindustrialpt_BR
dc.contributor(LBR) Lab. Biológicos Recombinantespt_BR
dc.contributorIniciação Científicapt_BR
dc.contributorCurso de Especialização em Biotecnologia para Saúde – Vacinas e Biofármacospt_BR
dc.contributor.authorMariano, Iago Henrique de Mirandapt_BR
dc.contributor.authorSilva, Bruna Ferreira dapt_BR
dc.contributor.authorMachado, Mayriele da Silvapt_BR
dc.contributor.authorBlanco, Roberta M.pt_BR
dc.contributor.authorRomero, Eliete C.pt_BR
dc.contributor.authorChudzinski, Sonia Aparecida de Andradept_BR
dc.contributor.authorHo, Paulo Leept_BR
dc.contributor.authorMartins, Elizabeth Angelica Lemept_BR
dc.contributor.authorSilva, Josefa Bezerra dapt_BR
dc.date.accessioned2023-02-14T18:30:16Z-
dc.date.available2023-02-14T18:30:16Z-
dc.date.issued2023pt_BR
dc.identifier.citationMariano IHM, Silva BF, Machado MS., Blanco RM., Romero EC., Chudzinski SAA, et al. Analysis of LruC lipoprotein and identification of peptides candidates for vaccine development and diagnosis of leptospirosis. PloS One. 2023 Feb; 18(2):e0281344. doi:10.1371/journal.pone.0281344.pt_BR
dc.identifier.urihttps://repositorio.butantan.gov.br/handle/butantan/4797-
dc.description.abstractLeptospirosis is a public health concern with lethality around 15% of the total cases. The current vaccines against Leptospira infection based on bacterins have several limitations, which require urgent development of new ones. In this context, groundbreaking approaches such as peptide-vaccines could be used to come around with promising results. Our goal was to identify conserved and immunogenic epitopes from the lipoprotein LruC that could interact with Major Histocompatibility Complex (MHC) I and II. LruC is a conserved lipoprotein expressed during leptospirosis that is considered among vaccine candidates and can be used as source for development of peptide-based vaccines. We searched for peptides that would be recognized by antibodies from either serum of hamsters previously immunized with low-LPS bacterin vaccines or from serum of patients diagnosed with leptospirosis. Immuno properties of seven peptides from LruC protein were evaluated in silico and by Dot Blot assay, and validate by ELISA. Preliminary results pointed one promising peptide that was recognized by the sera. In conclusion, the immunoinformatic approach helps the search and screening of peptides, while the Dot Blot assay, a simple and effective tool, helps to test and validate them. Thus, these prospective techniques together were validated to identify and validate potential peptides for further investigation as peptide-based vaccines or diagnostic methods.pt_BR
dc.description.sponsorship(FAPESP) Fundação de Amparo à Pesquisa do Estado de São Paulopt_BR
dc.description.sponsorship(CNPq) Conselho Nacional de Desenvolvimento Científico e Tecnológicopt_BR
dc.format.extente0281344pt_BR
dc.language.isoEnglishpt_BR
dc.relation.ispartofPloS Onept_BR
dc.rightsOpen accesspt_BR
dc.rights.urihttps://creativecommons.org/licenses/by/4.0/pt_BR
dc.titleAnalysis of LruC lipoprotein and identification of peptides candidates for vaccine development and diagnosis of leptospirosispt_BR
dc.typeArticlept_BR
dc.rights.licenseCC BYpt_BR
dc.identifier.doi10.1371/journal.pone.0281344pt_BR
dc.contributor.external(CNPq) Conselho Nacional de Desenvolvimento Científico e Tecnológicopt_BR
dc.contributor.external(IAL) Instituto Adolfo Lutzpt_BR
dc.identifier.citationvolume18pt_BR
dc.identifier.citationissue2pt_BR
dc.relation.ispartofabbreviatedPloS Onept_BR
dc.identifier.citationabntv. 18, n. 2, e0281344, fev. 2023pt_BR
dc.identifier.citationvancouver2023 Feb; 18(2):e0281344pt_BR
dc.contributor.butantanMariano, Iago Henrique de Miranda|:Aluno Egresso|:Curso de Especialização em Biotecnologia para Saúde – Vacinas e Biofármacos|:Lab. Bacteriologia|:Primeiro Autorpt_BR
dc.contributor.butantanSilva, Bruna Ferreira da|:Aluno Egresso|:Iniciação Científica|:Lab. Bacteriologia|:pt_BR
dc.contributor.butantanMachado, Mayriele da Silva|:Aluno|:Curso de Especialização em Biotecnologia para Saúde – Vacinas e Biofármacos|:Lab. Bacteriologia|:pt_BR
dc.contributor.butantanChudzinski, Sonia Aparecida de Andrade|:Pesquisador|:(BCA) Lab. Biofármacos|:pt_BR
dc.contributor.butantanHo, Paulo Lee|:Pesquisador|:Centro Bioindustrial|:pt_BR
dc.contributor.butantanElizabeth A. L. Martins|:Pesquisador|:(LBR) Lab. Biológicos Recombinantes|:pt_BR
dc.contributor.butantanSilva, Josefa Bezerra da|:Pesquisador|:Lab. Bacteriologia|:Autor de correspondência|:pt_BR
dc.sponsorship.butantan(FAPESP) Fundação de Amparo à Pesquisa do Estado de São Paulo¦¦2017/20903-6pt_BR
dc.sponsorship.butantan(CNPq) Conselho Nacional de Desenvolvimento Científico e Tecnológico¦¦pt_BR
dc.identifier.bvsccBR78.1pt_BR
dc.identifier.bvsdbIBProdpt_BR
dc.description.dbindexedYespt_BR
item.grantfulltextopen-
item.fulltextCom Texto completo-
item.languageiso639-1English-
item.openairetypeArticle-
crisitem.journal.journalissn#PLACEHOLDER_PARENT_METADATA_VALUE#-
crisitem.journal.journaleissn#PLACEHOLDER_PARENT_METADATA_VALUE#-
crisitem.author.dept#PLACEHOLDER_PARENT_METADATA_VALUE#-
crisitem.author.dept#PLACEHOLDER_PARENT_METADATA_VALUE#-
crisitem.author.dept#PLACEHOLDER_PARENT_METADATA_VALUE#-
crisitem.author.dept#PLACEHOLDER_PARENT_METADATA_VALUE#-
crisitem.author.dept#PLACEHOLDER_PARENT_METADATA_VALUE#-
crisitem.author.dept#PLACEHOLDER_PARENT_METADATA_VALUE#-
crisitem.author.dept#PLACEHOLDER_PARENT_METADATA_VALUE#-
crisitem.author.dept#PLACEHOLDER_PARENT_METADATA_VALUE#-
crisitem.author.dept#PLACEHOLDER_PARENT_METADATA_VALUE#-
crisitem.author.orcid#PLACEHOLDER_PARENT_METADATA_VALUE#-
crisitem.author.orcid#PLACEHOLDER_PARENT_METADATA_VALUE#-
crisitem.author.orcid#PLACEHOLDER_PARENT_METADATA_VALUE#-
crisitem.author.orcid#PLACEHOLDER_PARENT_METADATA_VALUE#-
crisitem.author.orcid#PLACEHOLDER_PARENT_METADATA_VALUE#-
crisitem.author.orcid0000-0003-4862-8131-
crisitem.author.orcid0000-0003-3652-241X-
crisitem.author.orcid0000-0002-2333-9379-
crisitem.author.orcid#PLACEHOLDER_PARENT_METADATA_VALUE#-
crisitem.author.parentorg#PLACEHOLDER_PARENT_METADATA_VALUE#-
crisitem.author.parentorg#PLACEHOLDER_PARENT_METADATA_VALUE#-
Appears in Collections:Artigos


Files in This Item:

Analysis of LruC lipoprotein and identification.pdf
Description:
Size: 2.23 MB
Format: Adobe PDF
View/Open
Show simple item record

This item is licensed under a Creative Commons License Creative Commons