Polymyxin Resistance in Salmonella: exploring mutations and genetic dDeterminants of non-human isolates

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dc.contributorLab. Bacteriologiapt_BR
dc.contributor.authorThais Vieirapt_BR
dc.contributor.authorCarla Adriana Dos Santospt_BR
dc.contributor.authorAmanda Maria de Jesus Bertanipt_BR
dc.contributor.authorCarvalho, Eneaspt_BR
dc.contributor.authorCosta, Alef Janguas dapt_BR
dc.date.accessioned2024-03-19T13:15:08Z-
dc.date.available2024-03-19T13:15:08Z-
dc.date.issued2024pt_BR
dc.identifier.urihttps://repositorio.butantan.gov.br/handle/butantan/5279-
dc.description.abstractUntil 2015, polymyxin resistance was primarily attributed to chromosomal mutations. However, with the first report of mobile colistin resistance (mcr-1) in commensal Escherichia coli from food animals in China, the landscape has changed. To evaluate the presence of polymyxin resistance in Salmonella spp., a drop screening test for colistin and polymyxin B was carried out on 1156 isolates of non-human origin (animals, food, and the environment), received in Brazil, between 2016 and 2021. Subsequently, 210 isolates with resistant results in the drop test were subjected to the gold-standard test (broth microdilution) for both colistin and polymyxin B. Whole-genome sequencing (WGS) of 102 resistant isolates was performed for a comprehensive analysis of associated genes. Surprisingly, none of the isolates resistant to colistin in the drop test harbored any of the mcr variants (mcr-1 to mcr-10). WGS identified that the most common mutations were found in pmrA (n= 22; T89S) and pmrB (n = 24; M15T, G73S, V74I, I83A, A111V). Other resistance determinants were also detected, such as the aac(6′)-Iaa gene in 72 isolates, while others carried beta-lactamase genes (blaTEM-1 blaCTX-M-2, blaCMY-2). Additionally, genes associated with fluoroquinolone resistance (qnrB19, qnrS1, oqxA/B) were detected in 11 isolates. Colistin and polymyxin B resistance were identified among Salmonella from non-human sources, but not associated with the mcr genes. Furthermore, the already-described mutations associated with polymyxin resistance were detected in only a small number of isolates, underscoring the need to explore and characterize unknown genes that contribute to resistance.pt_BR
dc.description.sponsorship(FAPESP) Fundação de Amparo à Pesquisa do Estado de São Paulopt_BR
dc.description.sponsorship(CNPq) Conselho Nacional de Desenvolvimento Científico e Tecnológicopt_BR
dc.format.extent110pt_BR
dc.language.isoEnglishpt_BR
dc.relation.ispartofAntibioticspt_BR
dc.rightsOpen accesspt_BR
dc.rights.urihttps://creativecommons.org/licenses/by/4.0/pt_BR
dc.titlePolymyxin Resistance in Salmonella: exploring mutations and genetic dDeterminants of non-human isolatespt_BR
dc.typeArticlept_BR
dc.rights.licenseCC BYpt_BR
dc.identifier.doi10.3390/antibiotics13020110pt_BR
dc.identifier.urlhttps://doi.org/10.3390/antibiotics13020110pt_BR
dc.contributor.external(IAL) Instituto Adolfo Lutzpt_BR
dc.contributor.external(USP) Universidade de São Paulopt_BR
dc.identifier.citationvolume13pt_BR
dc.identifier.citationissue2pt_BR
dc.subject.keywordSalmonella spppt_BR
dc.subject.keywordpolymyxinpt_BR
dc.subject.keywordcolistinpt_BR
dc.subject.keywordantimicrobial resistancept_BR
dc.relation.ispartofabbreviatedAntibioticspt_BR
dc.identifier.citationabntv. 13, n. 2, 110, jan. 2024pt_BR
dc.identifier.citationvancouver2024 Jan; 13(2):110pt_BR
dc.contributor.butantanCarvalho, Eneas|:Pesquisador|:Lab. Bacteriologiapt_BR
dc.sponsorship.butantan(FAPESP) Fundação de Amparo à Pesquisa do Estado de São Paulo¦¦2017/50333-7pt_BR
dc.sponsorship.butantan(FAPESP) Fundação de Amparo à Pesquisa do Estado de São Paulo¦¦2021/12219-3pt_BR
dc.sponsorship.butantan(CNPq) Conselho Nacional de Desenvolvimento Científico e Tecnológico¦¦402563/2021-2pt_BR
dc.identifier.bvsccBR78.1pt_BR
dc.identifier.bvsdbIBProdpt_BR
dc.description.dbindexedYespt_BR
item.fulltextCom Texto completo-
item.languageiso639-1English-
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item.openairetypeArticle-
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