Avaliação da toxicidade do muco do corpo dos bagres Pimelodus maculatus, Pinirampus pirinampu e Pterodoras granulosus em embriões de zebrafish


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Publication type
Academic monograph
Language
Portuguese
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Restricted access
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Abstract
Aquatic animals are associated with many cases of accidents on the Brazilian coast, especially fish, such as catfish. Accidents caused by venomous fish are characterized by intense pain, edema and erythema that progresses to necrosis that is difficult to heal. In addition to the venom produced by glands in the stingers, catfish mucus also contributes to poisoning. Among the venomous catfish studied so far, there are few studies on the envenomation caused by the Brazilian catfish P. maculatus, P. granulosus and P. pirinampu, especially the mucus that covers their bodies. The zebrafish (Danio rerio), popularly known as paulistinha, is used as an alternative model in research due to its physiological characteristics. The alternative validated zebrafish FET test OECD #236 is widely used in the analysis of potentially harmful substances, including venoms. The objective of this project is to study the possible toxic effects of mucus that covers the body of female and male catfish specimens P. maculatus, P. granulosus and P. pirinampu on zebrafish embryos using the embryo toxicity test to evaluate abnormalities in embryonic development. We found that a) a greater number of females were collected from March to August 2023, compared to males of the 3 species studied; b) mucus from females and males of P. granulosus and P. maculatus have similar protein content, but mucus from males of P. pirinampu has a higher protein content compared to mucus from females; c) mucus from females of the 3 species has a more diverse protein profile compared to mucus from males; the mucus of P. maculatus males has very few proteins, the mucus of P. granulosus males has proteins with a molecular weight of around 17 kDa and the mucus of P. granulosus females has the broadest protein repertoire with a weight between 3 and 62 kDa; d) mucus from P. granulosus females induces greater mortality than mucus from males, and mucus from males at low doses is not lethal; e) mucus from females and mucus from males of P. granulosus induce teratogenic (repertoire of 3 and 3, respectively) and mainly sublethal (4 and 7, respectively) abnormalities; f) low doses of mucus from P. pirinampu females induce a very high mortality rate and the very high rates induced by mucus from males are dose-dependent; g) mucus from P. pirinampu females induces a lower number of teratogenic abnormalities in relation to mucus from males (1 and 3, respectively) as well as sublethal abnormalities (5 and 8, respectively); h) the mucus of P. maculatus females induces very high mortality in a dose-dependent manner; the mortality induced by the mucus of females at the lowest dose (12.5 μg) is greater than the mortality induced by the mucus of males at the highest dose (10 μg) and i) the mucus of P. maculatus females does not induce abnormalities, but that of males induces a large repertoire of teratogenic and sublethal abnormalities (10). Taken together, we hope that our study on the toxic capacity of mucus from medically important Brazilian catfish (P. granulosus, P. pirinampu and P. maculatus) determined in an embryo-larval zebrafish model will allow a better understanding of envenomation and the pathways of signaling involved in the abnormalities paving the way for production of therapeutic compounds to treat the accidents and for the discovery of new target-directed molecules.
Reference
COSTA, Yasmin Stefanie Oliveira. Avaliação da toxicidade do muco do corpo dos bagres Pimelodus maculatus, Pinirampus pirinampu e Pterodoras granulosus em embriões de zebrafish. 2024. 71 p. Trabalho de Conclusão de Curso (Especialização em Toxinas de Interesse em Saúde) - Escola Superior do Instituto Butantan, São Paulo, 2024.
Link to cite this reference
https://repositorio.butantan.gov.br/handle/butantan/5345
Issue Date
2024

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