Estratégias para a manutenção reprodutiva de uma linhagem de camundongos obesos diabéticos: BKS.Cg - m+/+Leprdb/JUnib

Abstract

The study on the reproductive maintenance of the BKS. Cg-m+/+Leprdb/JUnib carried out at the Multidisciplinary Center for Biological Research in the area of Science in Laboratory Animals – CEMIB/UNICAMP, presented significant results for biomedical research related to obesity and type 2 diabetes. The general objective was to investigate strategies to optimize the reproduction of these mice, since the maintenance of the genetic characteristics of the strain is essential to obtain results and its reproducibility.To this end, the genotypes resulting from matings were analyzed, observing significant variations in the glycemic indexes throughout the development of the animals. Understanding influencing factors, such as genetics and environment, has allowed for the development of practical strategies, including the careful selection of parents based on their metabolic profiles. Data collection revealed a higher mean blood glucose in the colony resulting from the selection of matrices with higher glycemic indexes. Statistical analysis showed a progressive increase in glycemic indices over generations, demonstrating a pattern of heritability of these metabolic traits. The results obtained were discussed in the light of the previous literature, highlighting the importance of the targeted mating strategy in the maintenance of the studied lineage. Genetic analysis pointed to the influence of genetic inheritance on the expression of these metabolic traits, providing valuable insights for future research on obesity and type 2 diabetes in mice. In conclusion, the study contributed significantly to improve the knowledge about the reproductive maintenance of the BKS strain. Cg-m+/+Leprdb/JUnib, providing a strategies for the establishment of these colonies and more efficient management protocols in biomedical research es. These findings These findings the facility future studies related to obesity, type 2 diabetes, and other metabolic diseases, offering ne perspectives for understanding and treating these conditions in humans.