Chondroitin sulfate impairs neural stem cell migration through ROCK activation

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dc.contributorLaboratório de Fisiopatologiapt_BR
dc.contributorLaboratório de Biologia Molecularpt_BR
dc.contributor.authorGalindo, Layla T.pt_BR
dc.contributor.authorMundim, Mayara T. V. V.pt_BR
dc.contributor.authorPinto, Agnes S.pt_BR
dc.contributor.authorChiarantin, Gabrielly M. D.pt_BR
dc.contributor.authorAlmeida, Maíra Estanislau Soares dept_BR
dc.contributor.authorLamers, Marcelo L.pt_BR
dc.contributor.authorHorwitz, Alan R.pt_BR
dc.contributor.authorSantos, Marinilce F.pt_BR
dc.contributor.authorPorcionatto, Marimeliapt_BR
dc.date.accessioned2020-07-09T21:19:07Z-
dc.date.available2020-07-09T21:19:07Z-
dc.date.issued2018-
dc.identifier.citationGalindo LT., Mundim MT.V.V., Pinto AS., Chiarantin GM.D., Almeida MES, Lamers ML., et al. Chondroitin sulfate impairs neural stem cell migration through ROCK activation. Mol Neurobiol. 2018 Apr;55(4):3185-95. doi:10.1007/s12035-017-0565-8.pt_BR
dc.identifier.urihttps://repositorio.butantan.gov.br/handle/butantan/2398-
dc.description.abstractBrain injuries such as trauma and stroke lead to glial scar formation by reactive astrocytes which produce and secret axonal outgrowth inhibitors. Chondroitin sulfate proteoglycans (CSPG) constitute a well-known class of extracellular matrix molecules produced at the glial scar and cause growth cone collapse. The CSPG glycosaminoglycan side chains composed of chondroitin sulfate (CS) are responsible for its inhibitory activity on neurite outgrowth and are dependent on RhoA activation. Here, we hypothesize that CSPG also impairs neural stem cell migration inhibiting their penetration into an injury site. We show that DCX+ neuroblasts do not penetrate a CSPG-rich injured area probably due to Nogo receptor activation and RhoA/ROCK signaling pathway as we demonstrate in vitro with neural stem cells cultured as neurospheres and pull-down for RhoA. Furthermore, CS-impaired cell migration in vitro induced the formation of large mature adhesions and altered cell protrusion dynamics. ROCK inhibition restored migration in vitro as well as decreased adhesion size.pt_BR
dc.description.sponsorshipFundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)pt_BR
dc.description.sponsorshipConselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq)pt_BR
dc.description.sponsorshipNational Institute of General Medical Sciences (NIGMS)pt_BR
dc.format.extentp. 3185-3195pt_BR
dc.languageengpt_BR
dc.relation.ispartofMolecular Neurobiologypt_BR
dc.rightsOpen Accesspt_BR
dc.titleChondroitin sulfate impairs neural stem cell migration through ROCK activationpt_BR
dc.typeArticlept_BR
dc.identifier.doi10.1007/s12035-017-0565-8pt_BR
dc.identifier.urlhttp://dx.doi.org/10.1007/s12035-017-0565-8pt_BR
dc.contributor.externalUniversidade Federal de São Paulo (UNIFESP)¦¦Brasilpt_BR
dc.contributor.externalUniversidade Federal do Rio Grande do Sul (UFRGS)¦¦Brasilpt_BR
dc.contributor.externalUniversity of Virginia (UVA)¦¦Estados Unidospt_BR
dc.contributor.externalUniversidade de São Paulo (USP)¦¦Brasilpt_BR
dc.identifier.citationvolume55pt_BR
dc.identifier.citationissue4pt_BR
dc.subject.keywordNeural stem cellpt_BR
dc.subject.keywordCell migrationpt_BR
dc.subject.keywordChondroitinpt_BR
dc.subject.keywordTraumatic brain injurypt_BR
dc.subject.keywordRhoApt_BR
dc.subject.keywordRockpt_BR
dc.relation.ispartofabbreviatedMol Neurobiolpt_BR
dc.identifier.citationabntv. 55, n. 4, p. 3185-3195, abr. 2018pt_BR
dc.identifier.citationvancouver2018 Apr;55(4):3185-95pt_BR
dc.contributor.butantanAlmeida, Maíra Estanislau Soares de|:Aluno|:Laboratório de Fisiopatologia:Laboratório de Biologia Molecular|:pt_BR
dc.sponsorship.butantanConselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq)¦¦404,646/2012-3pt_BR
dc.sponsorship.butantanFundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)¦¦2011/00526-7pt_BR
dc.sponsorship.butantanFundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)¦¦2012/00652pt_BR
dc.sponsorship.butantanNational Institute of General Medical Sciences (NIGMS)¦¦GM23244pt_BR
dc.identifier.bvsccBR78.1pt_BR
dc.identifier.bvsdbIBProdpt_BR
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