Does the administration of pilocarpine prior to venom milking influence the composition of Micrurus corallinus venom?

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dc.contributorLab. Herpetologiapt_BR
dc.contributorCentro Bioindustrialpt_BR
dc.contributor.authorMorais-Zani, Karen dept_BR
dc.contributor.authorSilva, Caroline Serinopt_BR
dc.contributor.authorGalizio, Nathália da Costapt_BR
dc.contributor.authorTasima, Lidia Jorgept_BR
dc.contributor.authorPagotto, Josias Falararopt_BR
dc.contributor.authorRocha, Marisa Maria Teixeira dapt_BR
dc.contributor.authorMarcelino, José Robertopt_BR
dc.contributor.authorSant'Anna, Sávio Stefaninipt_BR
dc.contributor.authorTashima, Alexandre K.pt_BR
dc.contributor.authorTanaka-Azevedo, Anita Miticopt_BR
dc.contributor.authorGrego, Kathleen Fernandespt_BR
dc.date.accessioned2020-07-09T21:19:19Z-
dc.date.available2020-07-09T21:19:19Z-
dc.date.issued2018pt_BR
dc.identifier.citationMorais-Zani K, Silva CS, Galizio NC, Tasima LJ, Pagotto JF, Rocha MMT, et al. Does the administration of pilocarpine prior to venom milking influence the composition of Micrurus corallinus venom?. J. Proteomics. 2018 Mar;174:17-27. doi:10.1016/j.jprot.2017.12.010.pt_BR
dc.identifier.urihttps://repositorio.butantan.gov.br/handle/butantan/2412-
dc.description.abstractConsidering that the scarcity of venom represents a huge challenge for biochemical and functional studies of Micrurus species (coral snakes), in this report we describe for the first time the influence of pilocarpine administration prior to venom milking on the yield and protein composition of Micrurus corallinus venom. The administration of pilocarpine resulted in an increase of about 127% in the volume of venom milked, with similar protein content. Venoms showed similar protein bands distribution and intensity by SDS-PAGE and equivalents RP-HPLC profiles. Our proteomic analysis showed that venoms milked in the presence and absence of pilocarpine presented comparable protein profiles, in terms of protein composition and relative abundance. The toxins identified were assigned to 13 protein families and represent the most complete M. corallinus venom proteome described so far, in terms of number of protein families identified. Our data indicate that the administration of pilocarpine prior to venom milking increases the venom yield and does not change significantly the venom composition of M. corallinus. The employment of pilocarpine represents a useful approach to increase the yield of venom not only for Micrurus species, but also for other genera of snakes with limitations regarding the amount of venom available.pt_BR
dc.description.sponsorship(FAPESP) Fundação de Amparo à Pesquisa do Estado de São Paulopt_BR
dc.description.sponsorship(CAPES) Coordenação de Aperfeiçoamento de Pessoal de Nível Superiorpt_BR
dc.description.sponsorship(CNPq) Conselho Nacional de Desenvolvimento Científico e Tecnológicopt_BR
dc.description.sponsorship(FINEP) Financiadora de Estudos e Projetospt_BR
dc.format.extentp. 17-27pt_BR
dc.language.isoEnglishpt_BR
dc.relation.ispartofJournal of Proteomicspt_BR
dc.titleDoes the administration of pilocarpine prior to venom milking influence the composition of Micrurus corallinus venom?pt_BR
dc.typeArticlept_BR
dc.identifier.doi10.1016/j.jprot.2017.12.010pt_BR
dc.identifier.urlhttp://dx.doi.org/10.1016/j.jprot.2017.12.010pt_BR
dc.contributor.external(UNIFESP) Universidade Federal de São Paulo¦¦Brasilpt_BR
dc.contributor.external(USP) Universidade de São Paulopt_BR
dc.identifier.citationvolume174pt_BR
dc.subject.keywordMicrurus corallinuspt_BR
dc.subject.keywordPilocarpinept_BR
dc.subject.keywordVenom yieldpt_BR
dc.subject.keywordVenom compositionpt_BR
dc.relation.ispartofabbreviatedJ Proteomicspt_BR
dc.identifier.citationabntv. 174, p. 17-27, mar. 2018pt_BR
dc.identifier.citationvancouver2018 Mar;174:17-27pt_BR
dc.contributor.butantanSilva, Caroline Serino|:|:Lab. Herpetologia|:pt_BR
dc.contributor.butantanGalizio, Nathália da Costa|:Aluno|:Lab. Herpetologia|:pt_BR
dc.contributor.butantanTasima, Lidia Jorge|:Aluno|:Lab. Herpetologia|:pt_BR
dc.contributor.butantanRocha, Marisa Maria Teixeira da|:Técnico|:Lab. Herpetologia|:pt_BR
dc.contributor.butantanMarcelino, José Roberto|:Técnico|:Centro Bioindustrial|:pt_BR
dc.contributor.butantanSant'Anna, Sávio Stefanini|:Pesquisador|:Lab. Herpetologia|:pt_BR
dc.contributor.butantanTanaka-Azevedo, Anita Mitico|:Pesquisador|:Lab. Herpetologia|:pt_BR
dc.contributor.butantanGrego, Kathleen Fernandes|:Pesquisador|:Lab. Herpetologia|:Autor de correspondênciapt_BR
dc.contributor.butantanMorais-Zani, Karen de|:Pesquisador|:Lab. Herpetologia|:PrimeiroAutor:Autor de correspondênciapt_BR
dc.sponsorship.butantan(CNPq) Conselho Nacional de Desenvolvimento Científico e Tecnológico¦¦pt_BR
dc.sponsorship.butantan(CAPES) Coordenação de Aperfeiçoamento de Pessoal de Nível Superior¦¦pt_BR
dc.sponsorship.butantanFinanciadora de Estudos e Projetos (FINEP)¦¦pt_BR
dc.sponsorship.butantan(FAPESP) Fundação de Amparo à Pesquisa do Estado de São Paulo¦¦2014/11108-0pt_BR
dc.sponsorship.butantan(FAPESP) Fundação de Amparo à Pesquisa do Estado de São Paulo¦¦2016/09976-9pt_BR
dc.sponsorship.butantan(FAPESP) Fundação de Amparo à Pesquisa do Estado de São Paulo¦¦2017/01890-0pt_BR
dc.sponsorship.butantan(FAPESP) Fundação de Amparo à Pesquisa do Estado de São Paulo¦¦2012/19321-9pt_BR
dc.sponsorship.butantan(FAPESP) Fundação de Amparo à Pesquisa do Estado de São Paulo¦¦2016/03839-0pt_BR
dc.identifier.bvsccBR78.1pt_BR
dc.identifier.bvsdbIBProdpt_BR
dc.description.dbindexedYespt_BR
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