Mucosal immunization with PspA (Pneumococcal surface protein A)-adsorbed nanoparticles targeting the lungs for protection against pneumococcal infection

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dc.contributorLaboratório de Bacteriologiapt_BR
dc.contributorLaboratório Especial de Desenvolvimento de Vacinas (LEDV)pt_BR
dc.contributor.authorRodrigues, Tasson Costapt_BR
dc.contributor.authorOliveira, Maria Leonor Sarno dept_BR
dc.contributor.authorSchanoski, Alessandra Soarespt_BR
dc.contributor.authorRico, Stefanni Liliane Chavezpt_BR
dc.contributor.authorFigueiredo, Douglas Borgespt_BR
dc.contributor.authorGonçalves, Viviane Maimonipt_BR
dc.contributor.authorFerreira, Daniela M.pt_BR
dc.contributor.authorKunda, Nitesh K.pt_BR
dc.contributor.authorSaleem, Imran Y.pt_BR
dc.contributor.authorMiyaji, Eliane Namiept_BR
dc.date.accessioned2020-07-09T21:19:35Z-
dc.date.available2020-07-09T21:19:35Z-
dc.date.issued2018-
dc.identifier.citationRodrigues TC, Oliveira MLS, Schanoski AS, Rico SLC, Figueiredo DB, Gonçalves VM, et al. Mucosal immunization with PspA (Pneumococcal surface protein A)-adsorbed nanoparticles targeting the lungs for protection against pneumococcal infection. PLoS One. 2018 Jan;13(1):e0191692. doi:10.1371/journal.pone.0191692.pt_BR
dc.identifier.issn1932-6203-
dc.identifier.urihttps://repositorio.butantan.gov.br/handle/butantan/2430-
dc.description.abstractBurden of pneumonia caused by Streptococcus pneumoniae remains high despite the availability of conjugate vaccines. Mucosal immunization targeting the lungs is an attractive alternative for the induction of local immune responses to improve protection against pneumonia. Our group had previously described the development of poly(glycerol adipate-co-omega-pentadecalactone) (PGA-co-PDL) polymeric nanoparticles (NPs) adsorbed with Pneumococcal surface protein A from clade 4 (PspA4Pro) within L-leucine microcarriers (nanocomposite microparticles-NCMPs) for mucosal delivery targeting the lungs (NP/NCMP PspA4Pro). NP/NCMP PspA4Pro was now used for immunization of mice. Inoculation of this formulation induced anti-PspA4Pro IgG antibodies in serum and lungs. Analysis of binding of serum IgG to intact bacteria showed efficient binding to bacteria expressing PspA from clades 3, 4 and 5 (family 2), but no binding to bacteria expressing PspA from clades 1 and 2 (family 1) was observed. Both mucosal immunization with NP/NCMP PspA4Pro and subcutaneous injection of the protein elicited partial protection against intranasal lethal pneumococcal challenge with a serotype 3 strain expressing PspA from clade 5 (PspA5). Although similar survival levels were observed for mucosal immunization with NP/NCMP PspA4Pro and subcutaneous immunization with purified protein, NP/NCMP PspA4Pro induced earlier control of the infection. Conversely, neither immunization with NP/NCMP PspA4Pro nor subcutaneous immunization with purified protein reduced bacterial burden in the lungs after challenge with a serotype 19F strain expressing PspA from clade 1 (PspA1). Mucosal immunization with NP/NCMP PspA4Pro targeting the lungs is thus able to induce local and systemic antibodies, conferring protection only against a strain expressing PspA from the homologous family 2.pt_BR
dc.description.sponsorshipConselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq)pt_BR
dc.description.sponsorshipFundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)pt_BR
dc.description.sponsorshipCoordenação de Aperfeiçoamento de Pessoal de Nível Superior (CAPES)pt_BR
dc.format.extente0191692pt_BR
dc.languageengpt_BR
dc.publisherPublic Library Sciencept_BR
dc.relation.ispartofPloS Onept_BR
dc.rightsOpen Accesspt_BR
dc.titleMucosal immunization with PspA (Pneumococcal surface protein A)-adsorbed nanoparticles targeting the lungs for protection against pneumococcal infectionpt_BR
dc.typeArticlept_BR
dc.identifier.doi10.1371/journal.pone.0191692pt_BR
dc.identifier.urlhttp://dx.doi.org/10.1371/journal.pone.0191692pt_BR
dc.contributor.externalLiverpool School of Tropical Medicine (LSTM)¦¦Reino Unidopt_BR
dc.contributor.externalLiverpool John Moores University (LJMU)¦¦Reino Unidopt_BR
dc.contributor.externalUniversity of New Mexico¦¦Estados Unidospt_BR
dc.publisher.citySan Franciscopt_BR
dc.identifier.citationvolume13pt_BR
dc.identifier.citationissue1pt_BR
dc.relation.ispartofabbreviatedPLoS Onept_BR
dc.identifier.citationabntv. 13, n. 1, e0191692, jan. 2018pt_BR
dc.identifier.citationvancouver2018 Jan;13(1):e0191692pt_BR
dc.publisher.countryUnited Statespt_BR
dc.contributor.butantanOliveira, Maria Leonor Sarno de|:Pesquisador|:Laboratório de Bacteriologia|:pt_BR
dc.contributor.butantanSchanoski, Alessandra Soares|:Pesquisador|:Laboratório de Bacteriologia|:pt_BR
dc.contributor.butantanRico, Stefanni Liliane Chavez|:Aluno|:Laboratório de Bacteriologia|:pt_BR
dc.contributor.butantanFigueiredo, Douglas Borges|:Aluno|:Laboratório Especial de Desenvolvimento de Vacinas (LEDV)|:pt_BR
dc.contributor.butantanGonçalves, Viviane Maimoni|:Pesquisador|:Laboratório Especial de Desenvolvimento de Vacinas (LEDV)|:pt_BR
dc.contributor.butantanMiyaji, Eliane Namie|:Pesquisador|:Laboratório de Bacteriologia|:Autor de correspondênciapt_BR
dc.contributor.butantanRodrigues, Tasson Costa|:Aluno|:Laboratório de Bacteriologia|:PrimeiroAutorpt_BR
dc.sponsorship.butantanConselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq)¦¦303198/2014-1pt_BR
dc.sponsorship.butantanConselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq)¦¦140573/2015-1pt_BR
dc.sponsorship.butantanCoordenação de Aperfeiçoamento de Pessoal de Nível Superior (CAPES)¦¦pt_BR
dc.sponsorship.butantanFundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)¦¦2015/06255-6pt_BR
dc.sponsorship.butantanFundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)¦¦2014/25436-9pt_BR
dc.identifier.bvsccBR78.1pt_BR
dc.identifier.bvsdbIBProdpt_BR
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