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Large-scale evaluation of a rapid diagnostic test for diarrhea caused by enterotoxigenic Escherichia coli targeting the heat-labile toxin

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dc.contributorLab. Bacteriologiapt_BR
dc.contributor.authorHenrique, Camilapt_BR
dc.contributor.authorCaetano, Bruna Alvespt_BR
dc.contributor.authorMitsunari, Thaíspt_BR
dc.contributor.authordos Santos, Luis Fernandopt_BR
dc.contributor.authorPiazza, Roxane Maria Fontespt_BR
dc.contributor.authorRocha, Letícia Barbozapt_BR
dc.date.accessioned2020-07-09T21:19:44Z-
dc.date.available2020-07-09T21:19:44Z-
dc.date.issued2018pt_BR
dc.identifier.citationHenrique C, Caetano BA, Mitsunari T, dos Santos LF, Piazza RMF, Rocha LB. Large-scale evaluation of a rapid diagnostic test for diarrhea caused by enterotoxigenic Escherichia coli targeting the heat-labile toxin. J. Microbiol. Methods. 2018 Jan;144:125-7. doi:10.1016/j.mimet.2017.11.020.pt_BR
dc.identifier.urihttps://repositorio.butantan.gov.br/handle/butantan/2443-
dc.description.abstractWe standardized an immunochromatographic test (IC) for heat-labile toxin I (LT-I) detection using LT-I antibodies and a specific platform containing the apparatus for application, assembly and cutting. IC detected as little as 62.5 ng/mL of purified LT-I toxin and presented 91% sensitivity, 99.5% specificity and 96.0% accuracy, thereby proving to be an excellent point-of-care test for the diagnosis of enterotoxigenic E. coli infection in low-income countries.pt_BR
dc.description.sponsorship(FAPESP) Fundação de Amparo à Pesquisa do Estado de São Paulopt_BR
dc.description.sponsorship(CAPES) Coordenação de Aperfeiçoamento de Pessoal de Nível Superiorpt_BR
dc.description.sponsorship(CNPq) Conselho Nacional de Desenvolvimento Científico e Tecnológicopt_BR
dc.format.extentp. 125-127pt_BR
dc.language.isoEnglishpt_BR
dc.relation.ispartofJournal of Microbiological Methodspt_BR
dc.rightsOpen accesspt_BR
dc.titleLarge-scale evaluation of a rapid diagnostic test for diarrhea caused by enterotoxigenic Escherichia coli targeting the heat-labile toxinpt_BR
dc.typeArticlept_BR
dc.identifier.doi10.1016/j.mimet.2017.11.020pt_BR
dc.identifier.urlhttp://dx.doi.org/10.1016/j.mimet.2017.11.020pt_BR
dc.contributor.external(IAL) Instituto Adolfo Lutzpt_BR
dc.identifier.citationvolume144pt_BR
dc.subject.keywordETECpt_BR
dc.subject.keywordHeat-labile toxinpt_BR
dc.subject.keywordantibodiespt_BR
dc.subject.keywordImmunochromatographic testpt_BR
dc.subject.keyworddiagnosispt_BR
dc.relation.ispartofabbreviatedJ Microbiol Methodspt_BR
dc.identifier.citationabntv. 144, p. 125-127, jan. 2018pt_BR
dc.identifier.citationvancouver2018 Jan;144:125-7pt_BR
dc.contributor.butantanCaetano, Bruna Alves|:Aluno PPGTOX|:Lab. Bacteriologia|:pt_BR
dc.contributor.butantanMitsunari, Thaís|:Aluno|:Lab. Bacteriologia|:pt_BR
dc.contributor.butantanRocha, Letícia Barboza|:Aluno|:Lab. Bacteriologia|:pt_BR
dc.contributor.butantanHenrique, Camila|:Aluno PPGTOX|:Lab. Bacteriologia|:PrimeiroAutorpt_BR
dc.contributor.butantanPiazza, Roxane Maria Fontes|:Pesquisador:Docente Permanente PPGTOX|:Lab. Bacteriologia|:pt_BR
dc.sponsorship.butantan(CNPq) Conselho Nacional de Desenvolvimento Científico e Tecnológico¦¦301,302/2013-8pt_BR
dc.sponsorship.butantan(CAPES) Coordenação de Aperfeiçoamento de Pessoal de Nível Superior¦¦pt_BR
dc.sponsorship.butantan(FAPESP) Fundação de Amparo à Pesquisa do Estado de São Paulo¦¦2013/06589-6pt_BR
dc.sponsorship.butantan(FAPESP) Fundação de Amparo à Pesquisa do Estado de São Paulo¦¦2013/50955-7pt_BR
dc.sponsorship.butantan(FAPESP) Fundação de Amparo à Pesquisa do Estado de São Paulo¦¦2012/090906-2pt_BR
dc.identifier.bvsccBR78.1pt_BR
dc.identifier.bvsdbIBProdpt_BR
dc.description.dbindexedYespt_BR
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item.languageiso639-1English-
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