Transport of cowpea bean derived peptides and their modulator effects on mRNA expression of cholesterol-related genes in Caco-2 and HepG2 cells

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dc.contributorLaboratório de Bioquímica e Biofísicapt_BR
dc.contributor.authorMarques, Marcelo Rodriguespt_BR
dc.contributor.authorCerda, Alvaropt_BR
dc.contributor.authorFontanari, Gustavo Guadagnuccipt_BR
dc.contributor.authorPimenta, Daniel Carvalhopt_BR
dc.contributor.authorSoares-Freitas, Rosana Manoliopt_BR
dc.contributor.authorHirata, Mario Hiroyukipt_BR
dc.contributor.authorCrespo Hirata, Rosario Dominguezpt_BR
dc.contributor.authorGomes Areas, Jose Alfredopt_BR
dc.date.accessioned2020-07-09T21:19:54Z-
dc.date.available2020-07-09T21:19:54Z-
dc.date.issued2018-
dc.identifier.citationMarques MR, Cerda A, Fontanari GG, Pimenta DC, Soares-Freitas RM, Hirata MH, et al. Transport of cowpea bean derived peptides and their modulator effects on mRNA expression of cholesterol-related genes in Caco-2 and HepG2 cells. Food Res. Int.. 2018 May;107:165-71. doi:10.1016/j.foodres.2018.01.031.pt_BR
dc.identifier.issn0963-9969-
dc.identifier.urihttps://repositorio.butantan.gov.br/handle/butantan/2456-
dc.description.abstractThis work studied the cell transport of peptidase-generated peptides from cowpea bean proteins and their effects on mRNA expression of cholesterol-related genes in intestinal and liver cells. The <= 3 kDa hydrolysate was obtained and incubated with Caco-2 intestinal cells using Transwell center dot plates. HepG2 liver cells were incubated with synthetic analogues of peptides (MELNAVSVVHS and MELNAVSVVSH) identified by "de novo" peptide sequencing in the Caco-2 monolayer permeates. The mRNA expression of NPC1L1, ABCA1 and ABCG1 was measured in Caco-2 cells, in the presence or absence of <= 3 kDa hydrolysate and the expression of HMGCR, SREBP2, LXRa, AMPK1, was determined in the HepG2 cells in the presence or absence of synthetic peptides. Exposure of Caco-2 cells to cowpea <= 3 kDa hydrolysate (2.5 and 5 mg/mL) increased ABCG1 expression at 6 h and 12 h. SREBP2, HMGCR and LDLR mRNA levels were reduced in HepG2 cells after 24 h of treatment with MELNAVSVVHS peptide (50 mu M and 100 mu M). These results suggest that MELNAVSVVHS peptide is able to cross intestinal barrier and to modulate genes involved in cholesterol homeostasis.pt_BR
dc.description.sponsorshipFundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)pt_BR
dc.format.extentp. 165-171pt_BR
dc.languageengpt_BR
dc.publisherCanadian Institute of Food Science and Technologypt_BR
dc.relation.ispartofFood Research Internationalpt_BR
dc.titleTransport of cowpea bean derived peptides and their modulator effects on mRNA expression of cholesterol-related genes in Caco-2 and HepG2 cellspt_BR
dc.typeArticlept_BR
dc.identifier.doi10.1016/j.foodres.2018.01.031pt_BR
dc.identifier.urlhttp://dx.doi.org/10.1016/j.foodres.2018.01.031pt_BR
dc.contributor.externalUniversidade de São Paulo (USP)¦¦Brasilpt_BR
dc.contributor.externalUniversidad de La Frontera¦¦Chilept_BR
dc.contributor.externalInstituto Federal do Maranhão (IFMA)¦¦Brasilpt_BR
dc.publisher.cityOttawapt_BR
dc.identifier.citationvolume107pt_BR
dc.subject.keywordCowpea beanpt_BR
dc.subject.keywordHydrolysatept_BR
dc.subject.keywordPeptides uptakept_BR
dc.subject.keywordCholesterolpt_BR
dc.subject.keywordIntestinal barrierpt_BR
dc.relation.ispartofabbreviatedFood Res. Int.pt_BR
dc.identifier.citationabntv.107, p. 165-171, maio 2018pt_BR
dc.identifier.citationvancouver2018 May;107:165-71pt_BR
dc.publisher.countryCanadápt_BR
dc.contributor.butantanPimenta, Daniel Carvalho|:Pesquisador:Docente Permanente PPGTOX|:Laboratório de Bioquímica e Biofísica|:pt_BR
dc.sponsorship.butantanFundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)¦¦2013/09304-2pt_BR
dc.sponsorship.butantanFundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)¦¦2012/15900-4pt_BR
dc.identifier.bvsccBR78.1pt_BR
dc.identifier.bvsdbIBProdpt_BR
item.openairetypeArticle-
item.fulltextCom Texto completo-
item.grantfulltextembargo_29990101-
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