A functional and thromboelastometric-based micromethod for assessing crotoxin anticoagulant activity and antiserum relative potency against Crotalus durissus terrificus venom

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dc.contributorLab. Farmacologiapt_BR
dc.contributorLab. Herpetologiapt_BR
dc.contributorCentro Bioindustrialpt_BR
dc.contributorLaboratório de Ecologia e Evoluçãopt_BR
dc.contributor.authorPrezoto, Benedito Carlospt_BR
dc.contributor.authorTanaka-Azevedo, Anita Miticopt_BR
dc.contributor.authorMarcelino, José Robertopt_BR
dc.contributor.authorTashima, Alexandre K.pt_BR
dc.contributor.authorNishiduka, E. S.pt_BR
dc.contributor.authorKapronezai, Josanapt_BR
dc.contributor.authorMota, Jéssica de Oliveirapt_BR
dc.contributor.authorRocha, Marisa Maria Teixeira dapt_BR
dc.contributor.authorSilva, Caroline Serinopt_BR
dc.contributor.authorOguiura, Nancypt_BR
dc.date.accessioned2020-07-09T21:20:15Z-
dc.date.available2020-07-09T21:20:15Z-
dc.date.issued2018pt_BR
dc.identifier.citationPrezoto BC, Tanaka-Azevedo AM, Marcelino JR, Tashima AK., Nishiduka E.S., Kapronezai J, et al. A functional and thromboelastometric-based micromethod for assessing crotoxin anticoagulant activity and antiserum relative potency against Crotalus durissus terrificus venom. Toxicon. 2018 Jun;148:26-32. doi:10.1016/j.toxicon.2018.04.009.pt_BR
dc.identifier.urihttps://repositorio.butantan.gov.br/handle/butantan/2481-
dc.description.abstractThe assessment of the capacity of antivenoms to neutralize the lethal activity of snake venoms still relies on traditional rodent in vivo lethality assay. ED50 and LD50 assays require large quantities of venoms and antivenoms, and besides leading to animal suffering. Therefore, in vitro tests should be introduced for assessing antivenom neutralizing capacity in intermediary steps of antivenom production. This task is facilitated when one key lethal toxin is identified. A good example is crotoxin, a P-neurotoxin phospholipase A(2)-like toxin that presents anticoagulant activity in vitro and is responsible for the lethality of venoms of Crotalus durissus snakes. By using rotational thromboelastometry, we reported recently one sensitive coagulation assay for assessing relative potency of the anti-bothropic serum in neutralizing procoagulant activity of Bothrops jararaca venom upon recalcified factor-XII-deficient chicken plasma samples (CPS). In this study, we stablished conditions for determining relative potency of four batches of the anti-crotalic serum (ACS) (antagonist) in inactivating crotoxin anticoagulant activity in CPS (target) simultaneously treated with one classical activator of coagulation (agonists). The correlation coefficient (r) between values related the ACS potency in inactivating both in vitro crotoxin anticoagulant activity and the in vivo lethality of whole venom (ED50) was 0.94 (p value < 0.05). In conclusion, slowness in spontaneous thrombin/fibrin generation even after recalcification elicit time lapse sufficient for elaboration of one dose-response curve to pro-or anti-coagulant agonists in CPS. We propose this methodology as an alternative and sensitive assay for assessing antivenom neutralizing ability in plasma of immunized horses as well as for in-process quality control.pt_BR
dc.description.sponsorshipFundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)pt_BR
dc.format.extentp. 26-32pt_BR
dc.language.isoEnglishpt_BR
dc.relation.ispartofToxiconpt_BR
dc.titleA functional and thromboelastometric-based micromethod for assessing crotoxin anticoagulant activity and antiserum relative potency against Crotalus durissus terrificus venompt_BR
dc.typeArticlept_BR
dc.identifier.doi10.1016/j.toxicon.2018.04.009pt_BR
dc.identifier.urlhttp://dx.doi.org/10.1016/j.toxicon.2018.04.009pt_BR
dc.contributor.externalUniversidade Federal de São Paulo (UNIFESP)¦¦Brasilpt_BR
dc.identifier.citationvolume148pt_BR
dc.subject.keywordAntivenom neutralizationpt_BR
dc.subject.keywordCrotalus durissus terrificus venompt_BR
dc.subject.keywordCrotoxinpt_BR
dc.subject.keywordChicken plasmapt_BR
dc.subject.keywordRotational thromboelastometrypt_BR
dc.relation.ispartofabbreviatedToxiconpt_BR
dc.identifier.citationabntv. 148, p. 26-32, jun. 2018pt_BR
dc.identifier.citationvancouver2018 Jun;148:26-32pt_BR
dc.contributor.butantanTanaka-Azevedo, Anita Mitico|:Pesquisador|:Lab. Herpetologia|:pt_BR
dc.contributor.butantanMarcelino, José Roberto|:Técnico|:Centro Bioindustrial|:pt_BR
dc.contributor.butantanKapronezai, Josana|:Técnico|:Laboratório de Ecologia e Evolução|:pt_BR
dc.contributor.butantanMota, Jéssica de Oliveira|:Aluno|:Lab. Farmacologia|:pt_BR
dc.contributor.butantanRocha, Marisa Maria Teixeira da|:Técnico|:Lab. Herpetologia|:pt_BR
dc.contributor.butantanSilva, Caroline Serino|:Aluno|:Lab. Herpetologia|:pt_BR
dc.contributor.butantanOguiura, Nancy|:Pesquisador|:Laboratório de Ecologia e Evolução|:pt_BR
dc.contributor.butantanPrezoto, Benedito Carlos|:Pesquisador|:Lab. Farmacologia|:PrimeiroAutor:Autor de correspondênciapt_BR
dc.sponsorship.butantanFundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)¦¦2016/03839-0pt_BR
dc.sponsorship.butantanFundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)¦¦2017/01890-0pt_BR
dc.identifier.bvsccBR78.1pt_BR
dc.identifier.bvsdbIBProdpt_BR
dc.description.dbindexedYespt_BR
item.languageiso639-1English-
item.fulltextCom Texto completo-
item.openairetypeArticle-
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