Evaluation of Lsa46 and Lsa77 leptospiral proteins for their immunoprotective activities in hamster model of leptospirosis

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dc.contributor(LDV) Lab. Desenvolvimento de Vacinaspt_BR
dc.contributorPrograma de Pós-Graduação em Ciências – Toxinologia (PPGTox)pt_BR
dc.contributor.authorTeixeira, Aline Rodrigues Florênciopt_BR
dc.contributor.authorFernandes, Luis Guilherme Vírgíliopt_BR
dc.contributor.authorSouza Filho, Antôniopt_BR
dc.contributor.authorSouza, Gisele O. dept_BR
dc.contributor.authorVasconcellos, Silvio A.pt_BR
dc.contributor.authorHeinemann, Marcos B.pt_BR
dc.contributor.authorNascimento, Ana Lúcia Tabet Oller dopt_BR
dc.date.accessioned2020-07-09T21:20:18Z-
dc.date.available2020-07-09T21:20:18Z-
dc.date.issued2018pt_BR
dc.identifier.citationTeixeira ARF, Fernandes LGV, Souza Filho A, Souza GO., Vasconcellos SA., Heinemann MB., et al. Evaluation of Lsa46 and Lsa77 leptospiral proteins for their immunoprotective activities in hamster model of leptospirosis. Biomed Res. Int.. 2018;2018:1813745. doi:10.1155/2018/1813745.pt_BR
dc.identifier.urihttps://repositorio.butantan.gov.br/handle/butantan/2483-
dc.description.abstractLeptospirosis is a neglected tropical disease caused by pathogenic Leptospira spp. The lack of an effective vaccine favors the increase of the disease. Currently, surface-exposed proteins are the main targets for the search of vaccine candidates. In this study, we examined whether the surface Lsa46 and Lsa77 proteins, previously identified as laminin and plasminogen binding proteins, have the capacity of inducing protection and sterilizing immunity against challenge with virulent Leptospira in hamster model. Animals were subcutaneously immunized with Lsa46, Lsa77, or a combination of both in Alum adjuvant and challenged intraperitoneally with L. interrogans serovar Kennewicki strain Pomona Fromm. Hamster immunization with Lsa46 or Lsa77 or both promoted a strong IgG response. Th2- and Th1-biased immune responses were observed when Lsa46 and Lsa77 were individually administered, respectively, as detected by the IgG1/IgG2/3 ratio. Immunized hamsters with the combined proteins induced a Th1-biased immune response. Although the immunization with Lsa46 and Lsa77 stimulated protective immunity with reduction of bacterial burden, when compared to animals individually immunized with the proteins, the data was not statistically significant. Thus, although promising, more studies are needed before the role of these proteins in stimulating sterilizing immunity in mammals is conclusively determined.pt_BR
dc.description.sponsorship(FAPESP) Fundação de Amparo à Pesquisa do Estado de São Paulopt_BR
dc.description.sponsorship(CNPq) Conselho Nacional de Desenvolvimento Científico e Tecnológicopt_BR
dc.format.extent1813745pt_BR
dc.language.isoEnglishpt_BR
dc.relation.ispartofBioMed Research Internationalpt_BR
dc.rightsOpen accesspt_BR
dc.rights.urihttps://creativecommons.org/licenses/by/4.0/pt_BR
dc.titleEvaluation of Lsa46 and Lsa77 leptospiral proteins for their immunoprotective activities in hamster model of leptospirosispt_BR
dc.typeArticlept_BR
dc.rights.licenseCC BYpt_BR
dc.identifier.doi10.1155/2018/1813745pt_BR
dc.contributor.external(USP) Universidade de São Paulopt_BR
dc.identifier.citationvolume2018pt_BR
dc.relation.ispartofabbreviatedBiomed Res Intpt_BR
dc.identifier.citationabntv. 2018, 1813745, 2018pt_BR
dc.identifier.citationvancouver2018; 2018:1813745pt_BR
dc.contributor.butantanFernandes, Luis Guilherme Virgílio|:Aluno|:Programa de Pós-Graduação em Ciências – Toxinologia (PPGTox)|:(LDV) Lab. Desenvolvimento de Vacinas|:pt_BR
dc.contributor.butantanNascimento, Ana Lúcia Tabet Oller do|:Pesquisador|:(LDV) Lab. Desenvolvimento de Vacinas|:pt_BR
dc.contributor.butantanTeixeira, Aline Rodrigues Florêncio|:Aluno|:Programa de Pós-Graduação em Ciências – Toxinologia (PPGTox)|:(LDV) Lab. Desenvolvimento de Vacinas|:PrimeiroAutorpt_BR
dc.sponsorship.butantan(CNPq) Conselho Nacional de Desenvolvimento Científico e Tecnológico¦¦302758/2013-5pt_BR
dc.sponsorship.butantan(CNPq) Conselho Nacional de Desenvolvimento Científico e Tecnológico¦¦441449/2014-0pt_BR
dc.sponsorship.butantan(FAPESP) Fundação de Amparo à Pesquisa do Estado de São Paulo¦¦14/50981-0pt_BR
dc.sponsorship.butantan(FAPESP) Fundação de Amparo à Pesquisa do Estado de São Paulo¦¦2016/11541-0pt_BR
dc.sponsorship.butantan(FAPESP) Fundação de Amparo à Pesquisa do Estado de São Paulo¦¦2017/06731-8pt_BR
dc.identifier.bvsccBR78.1pt_BR
dc.identifier.bvsdbIBProdpt_BR
dc.description.dbindexedYespt_BR
item.fulltextCom Texto completo-
item.languageiso639-1English-
item.openairetypeArticle-
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