Antivenom production against Bothrops jararaca and Bothrops erythromelas snake venoms using cross-linked chitosan nanoparticles as an immunoadjuvant

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dc.contributorLaboratório de Imunoquímicapt_BR
dc.contributor.authorSoares, Karla Samara Rochapt_BR
dc.contributor.authorGláucia-Silva, Fiammapt_BR
dc.contributor.authorDaniele-Silva, Alessandrapt_BR
dc.contributor.authorTorres-Rêgo, Manoelapt_BR
dc.contributor.authorAraújo, Nathália Kelly dept_BR
dc.contributor.authorMenezes, Yamara Arruda Silva dept_BR
dc.contributor.authorDamasceno, Igor Zumbapt_BR
dc.contributor.authorTambourgi, Denise Vilarinhopt_BR
dc.contributor.authorSilva-Júnior, Arnóbio Antônio dapt_BR
dc.contributor.authorPedrosa, Matheus de Freitas Fernandespt_BR
dc.date.accessioned2020-07-09T21:20:22Z-
dc.date.available2020-07-09T21:20:22Z-
dc.date.issued2018-
dc.identifier.citationSoares KSR, Gláucia-Silva F, Daniele-Silva A, Torres-Rêgo M, Araújo NK, Menezes YAS, et al. Antivenom production against Bothrops jararaca and Bothrops erythromelas snake venoms using cross-linked chitosan nanoparticles as an immunoadjuvant. Toxins. 2018 Apr;10(4):158. doi:10.3390/toxins10040158.pt_BR
dc.identifier.urihttps://repositorio.butantan.gov.br/handle/butantan/2488-
dc.description.abstractIn Brazil, envenomation by snakes of the genus Bothrops is clinically relevant, particularly for the species Bothrops jararaca and B. erythromelas. The most effective treatment for envenomation by snakes is the administration of antivenoms associated with adjuvants. Novel adjuvants are required to reduce side effects and maximize the efficiency of conventional serum and vaccine formulations. The polymer chitosan has been shown to have immunoadjuvant properties, and it has been used as a platform for delivery systems. In this context, we evaluated the potential immunoadjuvant properties of chitosan nanoparticles (CNPs) loaded with B. jararaca and B. erythromelas venoms in the production of sera against these venoms. Stable CNPs were obtained by ionic gelation, and mice were immunized subcutaneously for 6 weeks with 100 mu L of each snake venom at concentrations of 5.0 or 10.0% (w/w), encapsulated in CNPs or associated with aluminium hydroxide (AH). The evaluation of protein interactions with the CNPs revealed their ability to induce antibody levels equivalent to those of AH, even with smaller doses of antigen. In addition, the CNPs were less inflammatory due to their modified release of proteins. CNPs provide a promising approach for peptide/protein delivery from snake venom and will be useful for new vaccines.pt_BR
dc.description.sponsorshipConselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq)pt_BR
dc.description.sponsorshipFundação de Apoio à Pesquisa do Rio Grande do Norte (FAPERN)pt_BR
dc.description.sponsorshipCoordenação de Aperfeiçoamento de Pessoal de Nível Superior (CAPES)pt_BR
dc.format.extent158pt_BR
dc.languageengpt_BR
dc.relation.ispartofToxinspt_BR
dc.rightsOpen Accesspt_BR
dc.titleAntivenom production against Bothrops jararaca and Bothrops erythromelas snake venoms using cross-linked chitosan nanoparticles as an immunoadjuvantpt_BR
dc.typeArticlept_BR
dc.identifier.doi10.3390/toxins10040158pt_BR
dc.identifier.urlhttp://dx.doi.org/10.3390/toxins10040158pt_BR
dc.contributor.externalUniversidade Federal do Rio Grande do Norte (UFRN)¦¦Brasilpt_BR
dc.identifier.citationvolume10pt_BR
dc.identifier.citationissue4pt_BR
dc.subject.keywordBothrops venomspt_BR
dc.subject.keywordantivenompt_BR
dc.subject.keywordadjuvantspt_BR
dc.subject.keywordnanoparticlespt_BR
dc.subject.keywordchitosanpt_BR
dc.subject.keywordnanovaccinespt_BR
dc.relation.ispartofabbreviatedToxinspt_BR
dc.identifier.citationabntv. 10, n. 4, 158, abr. 2018pt_BR
dc.identifier.citationvancouver2018 Apr;10(4):158pt_BR
dc.contributor.butantanTambourgi, Denise Vilarinho|:Pesquisador:Docente Permanente PPGTOX|:Laboratório de Imunoquímica|:pt_BR
dc.sponsorship.butantanConselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq)¦¦pt_BR
dc.sponsorship.butantanCoordenação de Aperfeiçoamento de Pessoal de Nível Superior (CAPES)¦¦(Toxinology/2010)pt_BR
dc.sponsorship.butantanFundação de Apoio à Pesquisa do Rio Grande do Norte (FAPERN)¦¦pt_BR
dc.identifier.bvsccBR78.1pt_BR
dc.identifier.bvsdbIBProdpt_BR
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