Co-localization of crotamine with internal membranes and accentuated accumulation in tumor cells

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dc.contributorLaboratório de Genéticapt_BR
dc.contributorLaboratório de Biologia Molecularpt_BR
dc.contributor.authorMambelli, Nicole Carolinept_BR
dc.contributor.authorSciani, Juliana Mozerpt_BR
dc.contributor.authorPrieto da Silva, Álvaro Rossan de Brandãopt_BR
dc.contributor.authorKerkis, Irinapt_BR
dc.identifier.citationMambelli NC, Sciani JM, Prieto da Silva ARB, Kerkis I. Co-localization of crotamine with internal membranes and accentuated accumulation in tumor cells. Molecules. 2018 Apr;23(4):968. doi:10.3390/molecules23040968.pt_BR
dc.description.abstractCrotamine is a highly cationic; cysteine rich, cross-linked, low molecular mass cell penetrating peptide (CPP) from the venom of the South American rattlesnake. Potential application of crotamine in biomedicine may require its large-scale purification. To overcome difficulties related with the purification of natural crotamine (nCrot) we aimed in the present study to synthesize and characterize a crotamine analog (sCrot) as well investigate its CPP activity. Mass spectrometry analysis demonstrates that sCrot and nCrot have equal molecular mass and biological function-the capacity to induce spastic paralysis in the hind limbs in mice. sCrot CPP activity was evaluated in a wide range of tumor and non-tumor cell tests performed at different time points. We demonstrate that sCrot-Cy3 showed distinct co-localization patterns with intracellular membranes inside the tumor and non-tumor cells. Time-lapse microscopy and quantification of sCrot-Cy3 fluorescence signalss in living tumor versus non-tumor cells revealed a significant statistical difference in the fluorescence intensity observed in tumor cells. These data suggest a possible use of sCrot as a molecular probe for tumor cells, as well as, for the selective delivery of anticancer molecules into these tumors.pt_BR
dc.description.sponsorshipFundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)pt_BR
dc.description.sponsorshipGlaxoSmithKline (GSK)pt_BR
dc.rightsOpen Accesspt_BR
dc.titleCo-localization of crotamine with internal membranes and accentuated accumulation in tumor cellspt_BR
dc.subject.keywordmolecular imagingpt_BR
dc.subject.keywordmembrane traffickingpt_BR
dc.subject.keywordcell penetrating peptide (CPP)pt_BR
dc.subject.keywordtumor markerpt_BR
dc.identifier.citationabntv. 23, n. 4, 968, abr. 2018pt_BR
dc.identifier.citationvancouver2018 Apr;23(4):968pt_BR
dc.contributor.butantanPrieto da Silva, Álvaro Rossan de Brandão|:Pesquisador|:Laboratório de Genética|:pt_BR
dc.contributor.butantanMambelli, Nicole Caroline|:Aluno|:Laboratório de Genética|:PrimeiroAutor:Autor de correspondênciapt_BR
dc.contributor.butantanSciani, Juliana Mozer|:Técnico:Docente Colaborador PPGTOX|:Laboratório de Biologia Molecular|:pt_BR
dc.contributor.butantanKerkis, Irina|:Pesquisador:Docente Permanente PPGTOX|:Laboratório de Genética|:pt_BR
dc.sponsorship.butantanFundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)¦¦pt_BR
dc.sponsorship.butantanGlaxoSmithKline (GSK)¦¦2015/50040-4pt_BR
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