Establishment of primary cell culture and an intracranial xenograft model of pediatric ependymoma: a prospect for therapy development and understanding of tumor biology

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dc.contributorLab. Imunogenéticapt_BR
dc.contributor(LDI) Laboratório de Desenvolvimento e Inovação Industrialpt_BR
dc.contributor.authorPavon, Lorena Favaropt_BR
dc.contributor.authorSibov, Tatiana Taispt_BR
dc.contributor.authorToledo, Silvia Regina Caminada dept_BR
dc.contributor.authorOliveira, Daniela Mara dept_BR
dc.contributor.authorCabral, Francisco Romeropt_BR
dc.contributor.authorSouza, Jean Gabriel dept_BR
dc.contributor.authorBoufleur, Pâmelapt_BR
dc.contributor.authorMarti, Luciana C.pt_BR
dc.contributor.authorMalheiros, Jackeline Moraespt_BR
dc.contributor.authorCruz, Edgar Ferreira dapt_BR
dc.contributor.authorPaiva, Fernando F.pt_BR
dc.contributor.authorMalheiros, Suzana M. F.pt_BR
dc.contributor.authorPaiva Neto, Manoel A. dept_BR
dc.contributor.authorTannús, Albertopt_BR
dc.contributor.authorOliveira, Sergio Mascarenhas dept_BR
dc.contributor.authorSilva, Nasjla Sabapt_BR
dc.contributor.authorCappellano, Andrea Mariapt_BR
dc.contributor.authorPetrilli, Antonio Sérgiopt_BR
dc.contributor.authorChudzinski-Tavassi, Ana Marisapt_BR
dc.contributor.authorCavalheiro, Sérgiopt_BR
dc.identifier.citationPavon LF, Sibov TT, Toledo SRC, Oliveira DM, Cabral FR, Souza JG, et al. Establishment of primary cell culture and an intracranial xenograft model of pediatric ependymoma: a prospect for therapy development and understanding of tumor biology. Oncotarget. 2018;9(31):21731-43. doi:10.18632/oncotarget.24932.pt_BR
dc.description.abstractBackground: Ependymoma (EPN), the third most common pediatric brain tumor, is a central nervous system (CNS) malignancy originating from the walls of the ventricular system. Surgical resection followed by radiation therapy has been the primary treatment for most pediatric intracranial EPNs. Despite numerous studies into the prognostic value of histological classification, the extent of surgical resection and adjuvant radiotherapy, there have been relatively few studies into the molecular and cellular biology of EPNs. Results: We elucidated the ultrastructure of the cultured EPN cells and characterized their profile of immunophenotypic pluripotency markers (CD133, CD90, SSEA-3, CXCR4). We established an experimental EPN model by the intracerebroventricular infusion of EPN cells labeled with multimodal iron oxide nanoparticles (MION), thereby generating a tumor and providing a clinically relevant animal model. MRI analysis was shown to be a valuable tool when combined with effective MION labeling techniques to accompany EPN growth. Conclusions: We demonstrated that GFAP/CD133+CD90+/CD44+ EPN cells maintained key histopathological and growth characteristics of the original patient tumor. The characterization of EPN cells and the experimental model could facilitate biological studies and preclinical drug screening for pediatric EPNs. Methods: In this work, we established notoriously challenging primary cell culture of anaplastic EPNs (WHO grade III) localized in the posterior fossa (PF), using EPNs obtained from 1 to 10-year-old patients (n = 07), and then characterized their immunophenotype and ultrastructure to finally develop a xenograft model.pt_BR
dc.format.extentp. 21731-21743pt_BR
dc.rightsOpen accesspt_BR
dc.titleEstablishment of primary cell culture and an intracranial xenograft model of pediatric ependymoma: a prospect for therapy development and understanding of tumor biologypt_BR
dc.rights.licenseCC BYpt_BR
dc.contributor.external(UNIFESP) Universidade Federal de São Paulopt_BR
dc.contributor.externalHospital Israelita Albert Einsteinpt_BR
dc.contributor.external(USP) Universidade de São Paulopt_BR
dc.contributor.external(GRAACC) Grupo de Apoio ao Adolescente e à Criança com Câncerpt_BR
dc.contributor.external(UNB) Universidade de Brasíliapt_BR
dc.subject.keywordprimary culture EPN cellspt_BR
dc.subject.keywordpluripotency markerspt_BR
dc.subject.keywordanimal modelpt_BR
dc.subject.keywordpreclinical studiespt_BR
dc.identifier.citationabntv. 9, n. 31, p. 21731-21743, 2018pt_BR
dc.contributor.butantanSouza, Jean Gabriel de|:Aluno|:Lab. Imunogenética|:pt_BR
dc.contributor.butantanBoufleur, Pâmela|:Aluno|:|:pt_BR
dc.contributor.butantanChudzinski-Tavassi, Ana Marisa|:Pesquisador:Docente Permanente PPGTOX|:Lab. Biologia Molecular|:pt_BR
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