Compositional and functional investigation of individual and pooled venoms from long-term captive and recently wild-caught Bothrops jararaca snakes

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dc.contributorLab. Herpetologiapt_BR
dc.contributorLab. Bacteriologiapt_BR
dc.contributor.authorGalizio, Nathália da Costapt_BR
dc.contributor.authorSilva, Caroline Serinopt_BR
dc.contributor.authorStuginski, Daniel Rodriguespt_BR
dc.contributor.authorAbreu, Patricia Antonia Estimapt_BR
dc.contributor.authorSant'Anna, Sávio Stefaninipt_BR
dc.contributor.authorGrego, Kathleen Fernandespt_BR
dc.contributor.authorTanaka-Azevedo, Anita Miticopt_BR
dc.contributor.authorMorais-Zani, Karen dept_BR
dc.date.accessioned2020-07-09T21:21:12Z-
dc.date.available2020-07-09T21:21:12Z-
dc.date.issued2018pt_BR
dc.identifier.citationGalizio NC, Silva CS, Stuginski DR, Abreu PAE, Sant'Anna SS, Grego KF, et al. Compositional and functional investigation of individual and pooled venoms from long-term captive and recently wild-caught Bothrops jararaca snakes. J. Proteomics. 2018 Aug;186:56-70. doi:10.1016/j.jprot.2018.07.007.pt_BR
dc.identifier.urihttps://repositorio.butantan.gov.br/handle/butantan/2552-
dc.description.abstractIntraspecific venom variability has been extensively reported in a number of species and is documented to be the result of several factors. However, current evidence for snake venom variability related to captivity maintenance is controversial. Here we report a compositional and functional investigation of individual and pooled venoms from long-term captive (LTC) and recently wild-caught (RWC) B. jararaca snakes. The composition of individual venoms showed a remarkable variability in terms of relative abundance of toxins (evidenced by 1-DE and RP-HPLC), enzymatic activities (proteolytic, PLA2, and LAAO) and coagulant activity, even among captive specimens. Thus, no compositional and functional pattern could be established to assign each individual venom to a specific group. Conversely, pooled venom from LTC and RWC snakes showed no significant differences regarding protein composition (characterized by 1-DE and shotgun proteomics), enzymatic activities (proteolytic, PLA2 and LAAO) and biological function (coagulant, hemorrhagic and lethal activities), except for edematogenic activity, which was more prominent in RWC venom pool. Additionally, both pooled venoms displayed similar immunoreactivity with the bothropic antivenom produced by Instituto Butantan. Taken together, our results highlight the complexity and the high intraspecific variation of B. jararaca venom, that is not influenced at a discernible extent by captivity maintenance. Biological significance: Bothrops jararaca snakes are one of the main causes of snakebites in Southeastern Brazil. Due to its medical interest, the venom of this species is the most studied and characterized among Brazilian snakes and captive B. jararaca specimens are maintained for long periods of time in our venom production facility. However, knowledge on the influence of captivity maintenance on B. jararaca venom variability is scarce. In this report, we described a high compositional and functional variability of individual venoms from LTC and RWC B. jararaca snakes, which are not observed between LTC and RWC pooled venoms. This intraspecific variability is more likely to be due to genetic/populational differences rather than "captivity vs wild" conditions. In this regard, data generated by the present work support the use of venom from captive and wild snakes for antivenom production and scientific research. Moreover, the data generated by this study highlight the importance of analyzing individual venom samples in studies involving intraspecific venom variability.pt_BR
dc.description.sponsorshipConselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq)pt_BR
dc.description.sponsorshipFundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)pt_BR
dc.description.sponsorshipCoordenação de Aperfeiçoamento de Pessoal de Nível Superior (CAPES)pt_BR
dc.format.extentp. 56-70pt_BR
dc.language.isoEnglishpt_BR
dc.relation.ispartofJournal of Proteomicspt_BR
dc.titleCompositional and functional investigation of individual and pooled venoms from long-term captive and recently wild-caught Bothrops jararaca snakespt_BR
dc.typeArticlept_BR
dc.identifier.doi10.1016/j.jprot.2018.07.007pt_BR
dc.identifier.urlhttps://doi.org/10.1016/j.jprot.2018.07.007pt_BR
dc.contributor.externalUniversidade de São Paulo (USP)¦¦Brasilpt_BR
dc.contributor.externalUniversidade Federal de São Paulo (UNIFESP)¦¦Brasilpt_BR
dc.identifier.citationvolume186pt_BR
dc.subject.keywordSnake venompt_BR
dc.subject.keywordIntraspecific venom variabilitypt_BR
dc.subject.keywordBothrops jararacapt_BR
dc.subject.keywordCaptivity maintenancept_BR
dc.subject.keywordVenom compositionpt_BR
dc.subject.keywordAntivenompt_BR
dc.relation.ispartofabbreviatedJ Proteomicspt_BR
dc.identifier.citationabntv. 186, p. 56-70, ago. 2018pt_BR
dc.identifier.citationvancouver2018 Aug;186:56-70pt_BR
dc.contributor.butantanSilva, Caroline Serino|:Aluno|:Lab. Herpetologia|:pt_BR
dc.contributor.butantanStuginski, Daniel Rodrigues|:Técnico|:Lab. Herpetologia|:pt_BR
dc.contributor.butantanAbreu, Patricia Antonia Estima|:Pesquisador|:Lab. Bacteriologia|:pt_BR
dc.contributor.butantanSant'Anna, Sávio Stefanini|:Pesquisador|:Lab. Herpetologia|:pt_BR
dc.contributor.butantanGrego, Kathleen Fernandes|:Pesquisador|:Lab. Herpetologia|:pt_BR
dc.contributor.butantanTanaka-Azevedo, Anita Mitico|:Pesquisador|:Lab. Herpetologia|:pt_BR
dc.contributor.butantanMorais-Zani, Karen de|:Pesquisador|:Lab. Herpetologia|:pt_BR
dc.contributor.butantanGalizio, Nathália da Costa|:Aluno|:Lab. Herpetologia|:PrimeiroAutorpt_BR
dc.sponsorship.butantanConselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq)¦¦pt_BR
dc.sponsorship.butantanCoordenação de Aperfeiçoamento de Pessoal de Nível Superior (CAPES)¦¦pt_BR
dc.sponsorship.butantanFundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)¦¦2014/11108-0pt_BR
dc.sponsorship.butantanFundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)¦¦2016/09976-9pt_BR
dc.sponsorship.butantanFundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)¦¦2017/01890-0pt_BR
dc.sponsorship.butantanFundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)¦¦2017/16908-2pt_BR
dc.sponsorship.butantanFundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)¦¦2016/03839-0pt_BR
dc.identifier.bvsccBR78.1pt_BR
dc.identifier.bvsdbIBProdpt_BR
dc.description.dbindexedYespt_BR
item.openairetypeArticle-
item.fulltextCom Texto completo-
item.grantfulltextembargo_29990101-
item.languageiso639-1English-
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