Analyses of the three 1-Cys Peroxiredoxins from Aspergillus fumigatus reveal that cytosolic Prx1 is central to H2O2 metabolism and virulence

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dc.contributorLab. Desenvolvimento de Vacinaspt_BR
dc.contributor.authorRocha, Marina Campospt_BR
dc.contributor.authorGodoy, Krissia Franco dept_BR
dc.contributor.authorBannitz-Fernandes, Renatapt_BR
dc.contributor.authorFabri, João H. T. Marilhanopt_BR
dc.contributor.authorBarbosa, Mayra Mara Ferraript_BR
dc.contributor.authorCastro, Patrícia Alves dept_BR
dc.contributor.authorAlmeida, Faustopt_BR
dc.contributor.authorGoldman, Gustavo Henriquept_BR
dc.contributor.authorCunha, Anderson Ferreira dapt_BR
dc.contributor.authorNetto, Luis E. S.pt_BR
dc.contributor.authorOliveira, Marcos Antonio dept_BR
dc.contributor.authorMalavazi, Iranpt_BR
dc.date.accessioned2020-07-09T21:21:17Z-
dc.date.available2020-07-09T21:21:17Z-
dc.date.issued2018pt_BR
dc.identifier.citationRocha MC, Godoy KF, Bannitz-Fernandes R, Fabri JH.T.M, Barbosa MMF, Castro PA, et al. Analyses of the three 1-Cys Peroxiredoxins from Aspergillus fumigatus reveal that cytosolic Prx1 is central to H2O2 metabolism and virulence. Sci Rep. 2018;8:12314. doi:10.1038/s41598-018-30108-2.pt_BR
dc.identifier.urihttps://repositorio.butantan.gov.br/handle/butantan/2560-
dc.description.abstractStanding among the front defense strategies against pathogens, host phagocytic cells release various oxidants. Therefore, pathogens have to cope with stressful conditions at the site of infection. Peroxiredoxins (Prx) are highly reactive and abundant peroxidases that can support virulence and persistence of pathogens in distinct hosts. Here, we revealed that the opportunistic human pathogen A. fumigatus presents three 1-Cys Prx (Prx6 subfamily), which is unprecedented. We showed that PrxB and PrxC were in mitochondria, while Prx1 was in cytosol. As observed for other Prxs, recombinant Prx1 and PrxC decomposed H2O2 at elevated velocities (rate constants in the 107?M-1s-1 range). Deletion mutants for each Prx displayed higher sensitivity to oxidative challenge in comparison with the wild-type strain. Additionally, cytosolic Prx1 was important for A. fumigatus survival upon electron transport dysfunction. Expression of Prxs was dependent on the SakAHOG1 MAP kinase and the Yap1YAP1 transcription factor, a global regulator of the oxidative stress response in fungi. Finally, cytosolic Prx1 played a major role in pathogenicity, since it is required for full virulence, using a neutropenic mouse infection model. Our data indicate that the three 1-Cys Prxs act together to maintain the redox balance of A. fumigatus.pt_BR
dc.description.sponsorship(FAPESP) Fundação de Amparo à Pesquisa do Estado de São Paulopt_BR
dc.description.sponsorship(CNPq) Conselho Nacional de Desenvolvimento Científico e Tecnológicopt_BR
dc.format.extent12314pt_BR
dc.language.isoEnglishpt_BR
dc.relation.ispartofScientific Reportspt_BR
dc.rightsOpen accesspt_BR
dc.rights.urihttps://creativecommons.org/licenses/by/4.0/pt_BR
dc.titleAnalyses of the three 1-Cys Peroxiredoxins from Aspergillus fumigatus reveal that cytosolic Prx1 is central to H2O2 metabolism and virulencept_BR
dc.typeArticlept_BR
dc.rights.licenseCC BYpt_BR
dc.identifier.doi10.1038/s41598-018-30108-2pt_BR
dc.identifier.urlhttps://doi.org/10.1038/s41598-018-30108-2pt_BR
dc.contributor.external(UFSCar) Universidade Federal de São Carlospt_BR
dc.contributor.external(USP) Universidade de São Paulopt_BR
dc.contributor.external(UNESP) Universidade Estadual Paulista Júlio de Mesquita Filhopt_BR
dc.identifier.citationvolume8pt_BR
dc.relation.ispartofabbreviatedSci Reppt_BR
dc.identifier.citationabntv. 8, 12314, 2018pt_BR
dc.identifier.citationvancouver2018;8:12314pt_BR
dc.contributor.butantanBarbosa, Mayra Mara Ferrari|:Aluno|:Laboratório de Desenvolvimento de Vacinas|:pt_BR
dc.sponsorship.butantan(CNPq) Conselho Nacional de Desenvolvimento Científico e Tecnológico¦¦462383/2014-8pt_BR
dc.sponsorship.butantan(FAPESP) Fundação de Amparo à Pesquisa do Estado de São Paulo¦¦2009/53546-5pt_BR
dc.sponsorship.butantan(FAPESP) Fundação de Amparo à Pesquisa do Estado de São Paulo¦¦2015/17541-0pt_BR
dc.sponsorship.butantan(FAPESP) Fundação de Amparo à Pesquisa do Estado de São Paulo¦¦2013/22375-6pt_BR
dc.sponsorship.butantan(FAPESP) Fundação de Amparo à Pesquisa do Estado de São Paulo¦¦2014/16636-4pt_BR
dc.sponsorship.butantan(FAPESP) Fundação de Amparo à Pesquisa do Estado de São Paulo¦¦2016/03322-7pt_BR
dc.identifier.bvsccBR78.1pt_BR
dc.identifier.bvsdbIBProdpt_BR
dc.description.dbindexedYespt_BR
item.fulltextCom Texto completo-
item.languageiso639-1English-
item.grantfulltextopen-
item.openairetypeArticle-
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