Sarconesin: sarconesiopsis magellanica blowfly larval excretions and secretions with antibacterial properties

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dc.contributorLETA - Laboratório de Toxinologia Aplicadapt_BR
dc.contributor.authorDíaz-Roa, Andreapt_BR
dc.contributor.authorPatarroyo, Manuel A.pt_BR
dc.contributor.authorBello, Felio J.pt_BR
dc.contributor.authorSilva Junior, Pedro Ismael dapt_BR
dc.identifier.citationDíaz-Roa A, Patarroyo MA., Bello FJ., Silva Junior PI. Sarconesin: Sarconesiopsis magellanica blowfly larval excretions and secretions with antibacterial properties. Front. Microbiol.. 2018;9:2249. doi:10.3389/fmicb.2018.02249.pt_BR
dc.description.abstractLarval therapy (LT) is an alternative treatment for healing chronic wounds; its action is based on debridement, the removal of bacteria, and stimulating granulation tissue. The most important mechanism when using LT for combating infection depends on larval excretions and secretions (ES). Larvae are protected against infection by a spectrum of antimicrobial peptides (AMPs); special interest in AMPs has also risen regarding understanding their role in wound healing since they degrade necrotic tissue and kill different bacteria during LT. Sarconesiopsis magellanica (Diptera: Calliphoridae) is a promising medically-important necrophagous fly. This article reports a small AMP being isolated from S. magellanica ES products for the first time; these products were obtained from third-instar larvae taken from a previously-established colony. ES were fractionated by RP-HPLC using C18 columns for the first analysis; the products were then lyophilised and their antimicrobial activity was characterized by incubation with different bacterial strains. These fractions’ primary sequences were determined by mass spectrometry and de novo sequencing; five AMPs were obtained, the Sarconesin fraction was characterized and antibacterial activity was tested in different concentrations with minimum inhibitory concentrations starting at 1.2 µM. Potent inhibitory activity was shown against Gram-negative (Escherichia coli D31, E. coli DH5a, Salmonella enterica ATCC 13314, Pseudomonas aeruginosa 27853) and Gram-positive (Staphylococcus aureus ATCC 29213, S. epidermidis ATCC 12228, Micrococcus luteus A270) bacteria. Sarconesin has a significant similarity with Rho-family GTPases which are important in organelle development, cytoskeletal dynamics, cell movement, and wound repair. The data reported here indicated that Sarconesin could be an alternative candidate for use in therapeutics against Gram-negative and Gram-positive bacterial infections. Our study describes one peptide responsible for antibacterial activity when LT is being used. The results shown here support carrying out further experiments aimed at validating S. magellanica AMPs as novel resources for combating antibacterial resistance.pt_BR
dc.description.sponsorshipDepartamento Administrativo de Ciencia, Tecnología e Innovación (Colciencias)pt_BR
dc.description.sponsorshipFundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)pt_BR
dc.description.sponsorshipConselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq)pt_BR
dc.relation.ispartofFrontiers in Microbiologypt_BR
dc.rightsOpen Accesspt_BR
dc.titleSarconesin: sarconesiopsis magellanica blowfly larval excretions and secretions with antibacterial propertiespt_BR
dc.contributor.externalUniversidad del Rosario¦¦Colômbiapt_BR
dc.contributor.externalUniversidade de São Paulo (USP)¦¦Brasilpt_BR
dc.contributor.externalFundación Instituto de Inmunología de Colombia (FIDIC)¦¦Colômbiapt_BR
dc.contributor.externalUniversidad de La Salle¦¦Colômbiapt_BR
dc.contributor.externalUniversidad Antonio Nariño¦¦Colômbiapt_BR
dc.subject.keywordantimicrobial peptidept_BR
dc.subject.keywordSarconesiopsis magellanicapt_BR
dc.subject.keywordlarval therapypt_BR
dc.subject.keywordinsect peptidept_BR
dc.relation.ispartofabbreviatedFront Microbiolpt_BR
dc.identifier.citationabntv. 9, 2249, 2018pt_BR
dc.contributor.butantanSilva Junior, Pedro Ismael da|:Pesquisador|:Laboratório Especial de Toxinologia Aplicada (LETA)|:Autor de correspondênciapt_BR
dc.contributor.butantanDíaz-Roa, Andrea|:Aluno|:Laboratório Especial de Toxinologia Aplicada (LETA)|:PrimeiroAutorpt_BR
dc.sponsorship.butantanConselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq)¦¦472744/2012-7pt_BR
dc.sponsorship.butantanDepartamento Administrativo de Ciencia, Tecnología e Innovación (Colciencias)¦¦FP44842-384-2016pt_BR
dc.sponsorship.butantanDepartamento Administrativo de Ciencia, Tecnología e Innovación (Colciencias)¦¦125371250687pt_BR
dc.sponsorship.butantanFundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)¦¦13/07467-1pt_BR
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