Effect of chaotropes in Chagas disease and leishmaniasis cross-reacting serology assays for epidemiological surveys

INTRODUCTION: Serological cross-reactivity between leishmaniasis and Chagas disease, especially at low titers, leads to difficulties of the seroepidemiological interpretation. METHODS: We have studied the ability of urea as a chaotrope to select high-avidity antibodies in IgG ELISA, thus reducing low-avidity IgG cross-reactivity in serologically positive samples in both assays. RESULTS: Using 0.5M urea for diluting the sample efficiently defined leishmaniasis or double infections in high-avidity IgG ELISA and eliminated false-positive results. CONCLUSIONS: The use of a chaotropic diluting agent is useful for improving the specificity of Chagas disease and leishmaniasis immunoassays.
Keywords
Serology;  Chagas disease;  Leishmaniasis;  ELISA;  IFAT;  IgG antibody avidity

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Pacheco AS, Carvalho ME, Meirelles LR, Andrade Junior HF. Effect of chaotropes in Chagas disease and leishmaniasis cross-reacting serology assays for epidemiological surveys. Rev. Soc. Bras. Med. Trop.. 2018 Oct;51(5):665-9. doi:10.1590/0037-8682-0391-2017.
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