Effect of chaotropes in Chagas disease and leishmaniasis cross-reacting serology assays for epidemiological surveys
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INTRODUCTION: Serological cross-reactivity between leishmaniasis and Chagas disease, especially at low titers, leads to difficulties of the seroepidemiological interpretation. METHODS: We have studied the ability of urea as a chaotrope to select high-avidity antibodies in IgG ELISA, thus reducing low-avidity IgG cross-reactivity in serologically positive samples in both assays. RESULTS: Using 0.5M urea for diluting the sample efficiently defined leishmaniasis or double infections in high-avidity IgG ELISA and eliminated false-positive results. CONCLUSIONS: The use of a chaotropic diluting agent is useful for improving the specificity of Chagas disease and leishmaniasis immunoassays.
Pacheco AS, Carvalho ME, Meirelles LR, Andrade Junior HF. Effect of chaotropes in Chagas disease and leishmaniasis cross-reacting serology assays for epidemiological surveys. Rev. Soc. Bras. Med. Trop.. 2018 Oct;51(5):665-9. doi:10.1590/0037-8682-0391-2017.
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