Model comparison to describe BHK-21 cell growth and metabolism in stirred tank bioreactors operated in batch mode

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dc.contributorLaboratório de Imunologia Viralpt_BR
dc.contributorLaboratório Piloto de Imunobiológicos Veterináriospt_BR
dc.contributor.authorSartori, Rogerpt_BR
dc.contributor.authorLeme, Jacipt_BR
dc.contributor.authorCaricati, Celso Pereirapt_BR
dc.contributor.authorTonso, Aldopt_BR
dc.contributor.authorFernández Núñez, Eutimio Gustavopt_BR
dc.identifier.citationSartori R, Leme J, Caricati CP, Tonso A, Fernández Núñez EG. Model comparison to describe BHK-21 cell growth and metabolism in stirred tank bioreactors operated in batch mode. Braz. J. Chem. Eng.. 2018 Jun;35(2):441-58. doi:10.1590/0104-6632.20180352s20160592.pt_BR
dc.description.abstractBaby Hamster Kidney cells (BHK-21) are commonly used in research and the biopharmaceutical industry. This work aimed to model the kinetic performance in batch operation mode of BHK-21 cells cultured in two stirred tank configurations using different dissolved oxygen concentrations and pH control strategies. Viable and dead cell concentrations, as well as glucose, glutamine, lactate and ammonium concentrations, were monitored. Statistical multiple linear regression, logistic equation and multiplicative Monod kinetic models were fitted. Statistical models for viable cells concentration as a function of nutrient and metabolite concentrations were significant (R2 >0.91). Logistic model parameters: intrinsic growth rate, cell density level in the medium and time for reaching maximum cell concentrations were within 0.061-0.083 h-1, 1.85-5.39 x 109 cell L-1 and 52-90 h ranges, respectively. A Monod-type model was satisfactorily fitted to the experimental data. Relative errors were lower than 10% for six monitored state variables in most of the assessed experimental conditions. The three models developed in this work can be used in bioprocesses involving BHK-21 with good fitting.pt_BR
dc.description.sponsorshipFundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)pt_BR
dc.description.sponsorshipConselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq)pt_BR
dc.format.extentp. 441-458pt_BR
dc.publisherBrazilian Society of Chemical Engineeringpt_BR
dc.relation.ispartofBrazilian Journal of Chemical Engineeringpt_BR
dc.rightsOpen Accesspt_BR
dc.titleModel comparison to describe BHK-21 cell growth and metabolism in stirred tank bioreactors operated in batch modept_BR
dc.contributor.externalUniversidade Estadual Paulista Júlio de Mesquita Filho (UNESP)¦¦Brasilpt_BR
dc.contributor.externalUniversidade de São Paulo (USP)¦¦Brasilpt_BR
dc.contributor.externalUniversidade Federal do ABC (UFABC)¦¦Brasilpt_BR
dc.publisher.citySão Paulopt_BR
dc.subject.keywordbatch operationpt_BR
dc.subject.keywordbioprocess engineeringpt_BR
dc.subject.keywordmammalian cell culturept_BR
dc.subject.keywordmathematical modellingpt_BR
dc.subject.keywordstirred tank bioreactorpt_BR
dc.relation.ispartofabbreviatedBraz. J. Chem. Eng.pt_BR
dc.identifier.citationabntv. 35, n. 2, p. 441-458pt_BR
dc.identifier.citationvancouver2018 Jun;35(2):441-58pt_BR
dc.contributor.butantanCaricati, Celso Pereira|:Pesquisador|:Laboratório Piloto de Imunobiológicos Veterinários|:pt_BR
dc.contributor.butantanLeme, Jaci|:Técnico|:Laboratório de Imunologia Viral:Laboratório Piloto de Imunobiológicos Veterinários|:pt_BR
dc.sponsorship.butantanConselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq)¦¦483009/2010-5pt_BR
dc.sponsorship.butantanFundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)¦¦2010/52521-6pt_BR
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