IgG induced by vaccination with Ascaris suum extracts is protective against infection

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dc.contributorLab. Bacteriologiapt_BR
dc.contributorCentro Bioindustrialpt_BR
dc.contributor.authorGazzinelli-Guimarães, Ana Clarapt_BR
dc.contributor.authorGazzinelli-Guimarães, Pedro Henriquept_BR
dc.contributor.authorNogueira, Denise Silvapt_BR
dc.contributor.authorOliveira, Fabrício Marcus Silvapt_BR
dc.contributor.authorBarbosa, Fernando Sérgiopt_BR
dc.contributor.authorAmorim, Chiara Cássia Oliveirapt_BR
dc.contributor.authorCardoso, Mariana Santospt_BR
dc.contributor.authorKraemer, Lucas Rochapt_BR
dc.contributor.authorCaliari, Marcelo Vidigalpt_BR
dc.contributor.authorAkamatsu, Milena Apetitopt_BR
dc.contributor.authorHo, Paulo Leept_BR
dc.contributor.authorJones, Kathryn M.pt_BR
dc.contributor.authorWeatherhead, Jill E.pt_BR
dc.contributor.authorBottazzi, Maria Elenapt_BR
dc.contributor.authorHotez, Peter J.pt_BR
dc.contributor.authorZhan, Binpt_BR
dc.contributor.authorBartholomeu, Daniella Castanheirapt_BR
dc.contributor.authorRusso, Remo Castropt_BR
dc.contributor.authorBueno, Lílian Lacerdapt_BR
dc.contributor.authorFujiwara, Ricardo Toshiopt_BR
dc.date.accessioned2020-07-09T21:21:57Z-
dc.date.available2020-07-09T21:21:57Z-
dc.date.issued2018pt_BR
dc.identifier.citationGazzinelli-Guimarães AC, Gazzinelli-Guimarães PH, Nogueira DS, Oliveira FMS, Barbosa FS, Amorim CCO, et al. IgG induced by vaccination with Ascaris suum extracts is protective against infection. Front. Immunol.. 2018;9:2535. doi:10.3389/fimmu.2018.02535.pt_BR
dc.identifier.urihttps://repositorio.butantan.gov.br/handle/butantan/2606-
dc.description.abstractHuman ascariasis has a global and cosmopolitan distribution, and has been characterized as the most prevalent neglected tropical disease worldwide. The development of a preventive vaccine is highly desirable to complement current measures required for this parasitic infection control and to reduce chronic childhood morbidities. In the present study, we describe the mechanism of protection elicited by a preventive vaccine against ascariasis. Vaccine efficacy was evaluated after immunization with three different Ascaris suum antigen extracts formulated with monophosphoryl lipid A (MPLA) as an adjuvant: crude extract of adult worm (ExAD); crude extract of adult worm cuticle (CUT); and crude extract of infective larvae (L3) (ExL3). Immunogenicity elicited by immunization was assessed by measuring antibody responses, cytokine production, and influx of tissue inflammatory cells. Vaccine efficacy was evaluated by measuring the reductions in the numbers of larvae in the lungs of immunized BALB/c mice that were challenged with A. suum eggs. Moreover, lung physiology and functionality were tested by spirometry to determine clinical efficacy. Finally, the role of host antibody mediated protection was determined by passive transfer of serum from immunized mice. Significant reductions in the total number of migrating larvae were observed in mice immunized with ExL3 61% (p < 0.001), CUT 59% (p < 0.001), and ExAD 51% (p < 0.01) antigens in comparison with non-immunized mice. For the Ascaris antigen-specific IgG antibody levels, a significant and progressive increase was observed with each round of immunization, in association with a marked increase of IgG1 and IgG3 subclasses. Moreover, a significant increase in concentration of IL-5 and IL-10 (pre-challenge) in the blood and IL-10 in the lung tissue (post-challenge) was induced by CUT immunization. Finally, ExL3 and CUT-immunized mice showed a marked improvement in lung pathology and tissue fibrosis as well as reduced pulmonary dysfunction induced by Ascaris challenge, when compared to non-immunized mice. Moreover, the passive transfer of specific IgG antibodies from ExL3, CUT, and ExAD elicited a protective response in naïve mice, with significant reductions in parasite burdens in lungs of 65, 64, and 64%, respectively. Taken together, these studies indicated that IgG antibodies contribute to protective immunity.pt_BR
dc.description.sponsorship(FAPEMIG) Fundação de Amparo à Pesquisa do Estado de Minas Geraispt_BR
dc.description.sponsorship(CNPq) Conselho Nacional de Desenvolvimento Científico e Tecnológicopt_BR
dc.description.sponsorship(CAPES) Coordenação de Aperfeiçoamento de Pessoal de Nível Superiorpt_BR
dc.format.extent2535pt_BR
dc.language.isoEnglishpt_BR
dc.relation.ispartofFrontiers in Immunologypt_BR
dc.rightsOpen accesspt_BR
dc.rights.urihttps://creativecommons.org/licenses/by/4.0/pt_BR
dc.titleIgG induced by vaccination with Ascaris suum extracts is protective against infectionpt_BR
dc.typeArticlept_BR
dc.rights.licenseCC BYpt_BR
dc.identifier.doi10.3389/fimmu.2018.02535pt_BR
dc.identifier.urlhttps://doi.org/10.3389/fimmu.2018.02535pt_BR
dc.contributor.external(UFMG) Universidade Federal de Minas Geraispt_BR
dc.contributor.externalBaylor College of Medicinept_BR
dc.identifier.citationvolume9pt_BR
dc.subject.keywordAscarispt_BR
dc.subject.keywordvaccinept_BR
dc.subject.keywordantigenspt_BR
dc.subject.keywordimmune responsept_BR
dc.subject.keywordhumoral responsept_BR
dc.relation.ispartofabbreviatedFront Immunolpt_BR
dc.identifier.citationabntv. 9, 2535, 2018pt_BR
dc.identifier.citationvancouver2018;9:2535pt_BR
dc.contributor.butantanAkamatsu, Milena Apetito|:Pesquisador|:Centro Bioindustrial|:pt_BR
dc.contributor.butantanHo, Paulo Lee|:Pesquisador|:Centro Bioindustrial|:pt_BR
dc.sponsorship.butantan(CNPq) Conselho Nacional de Desenvolvimento Científico e Tecnológico¦¦479613/2012-5pt_BR
dc.sponsorship.butantan(CNPq) Conselho Nacional de Desenvolvimento Científico e Tecnológico¦¦478729/2011-1pt_BR
dc.sponsorship.butantan(CAPES) Coordenação de Aperfeiçoamento de Pessoal de Nível Superior¦¦pt_BR
dc.sponsorship.butantanFundação de Amparo à Pesquisa do Estado de Minas Gerais (FAPEMIG)¦¦APQ-01202-09pt_BR
dc.sponsorship.butantanFundação de Amparo à Pesquisa do Estado de Minas Gerais (FAPEMIG)¦¦APQ-02167-13pt_BR
dc.sponsorship.butantanFundação de Amparo à Pesquisa do Estado de Minas Gerais (FAPEMIG)¦¦PPM-00296-11pt_BR
dc.sponsorship.butantanFundação de Amparo à Pesquisa do Estado de Minas Gerais (FAPEMIG)¦¦APQ03280-15pt_BR
dc.identifier.bvsccBR78.1pt_BR
dc.identifier.bvsdbIBProdpt_BR
dc.description.dbindexedYespt_BR
item.languageiso639-1English-
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