Influence of different processing techniques on the toxicity and biochemical characteristics of Tityus serrulatus scorpion venom

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dc.contributorLab. Farmacologiapt_BR
dc.contributorLab. Herpetologiapt_BR
dc.contributorLaboratório de Artrópodespt_BR
dc.contributorLab. Bioquímicapt_BR
dc.contributorLab. Imunopatologiapt_BR
dc.contributor.authorDorce, Valquiria Abrão Coronadopt_BR
dc.contributor.authorRocha, Marisa Maria Teixeira dapt_BR
dc.contributor.authorCandido, Denise Mariapt_BR
dc.contributor.authorNencioni, Ana Leonor Abrahãopt_BR
dc.contributor.authorAuada, Aline Vivian Vattipt_BR
dc.contributor.authorBarbaro, Katia Cristinapt_BR
dc.contributor.authorLebrun, Ivopt_BR
dc.identifier.citationDorce VAC, Rocha MMT, Candido DM, Nencioni ALA, Auada AVV, Barbaro KC, et al. Influence of different processing techniques on the toxicity and biochemical characteristics of Tityus serrulatus scorpion venom. Toxicon. 2018;156:41-7. doi:10.1016/j.toxicon.2018.11.004.pt_BR
dc.description.abstractStudies of scorpion venoms have used different venom drying methods: lyophilization, desiccation, lyophilization after mixing with 0.9% saline or purified water and centrifugation. The aim of this study was to see if these different approaches cause some alteration in the composition of the venom or interfere with its biological effects. Mice were injected (i.p.) with T. serrulatus scorpion venom in the liquid form (G-liq) or dried by different methods (lyophilized – G-lyo; centrifuged and the supernatant lyophilized – G-cen; desiccated – G-des), and observed regarding the occurrence of the symptoms respiratory difficulty, convulsion and death. The occurrence of seizures, although occurring in all groups and with the various doses used, did not prove to be effective to determine differences between the different handling techniques. Respiratory distress appeared to be useful in analyzing differences between groups, where this effect was less pronounced in the G-liq and G-des groups. In general, death occurred in a certain proportion with increasing dose for all groups. G-liq and G-des seemed to be more "active" at lower doses and G-cen and G-lyo at higher doses. The electrophoretic and chromatographic profile demonstrated main differences between G-liq and the dried groups. In the electrophoretic profile, the liquid venom showed bands of proteins of higher concentration and greater number of major bands and the three dried venom had the lowest number of protein bands. The HPLC profile and densitometry of the electrophoretic profiles showed some differences that may be associated with different protein conformation/aggregation. Our data indicated that lyophilization is the most suitable method for processing T. serrulatus scorpion venom after extraction.pt_BR
dc.description.sponsorship(CAPES) Coordenação de Aperfeiçoamento de Pessoal de Nível Superiorpt_BR
dc.description.sponsorship(FAPESP) Fundação de Amparo à Pesquisa do Estado de São Paulopt_BR
dc.titleInfluence of different processing techniques on the toxicity and biochemical characteristics of Tityus serrulatus scorpion venompt_BR
dc.subject.keywordscorpion venompt_BR
dc.subject.keywordTityus serrulatuspt_BR
dc.subject.keywordVenom processingpt_BR
dc.identifier.citationabntv. 156, p. 41-47, 2018pt_BR
dc.contributor.butantanRocha, Marisa Maria Teixeira da|:Colaborador|:Lab. Herpetologia|:pt_BR
dc.contributor.butantanCandido, Denise Maria|:Colaborador|:Laboratório de Artrópodes|:pt_BR
dc.contributor.butantanAuada, Aline Vivian Vatti|:Aluno|:Lab. Bioquímica|:pt_BR
dc.contributor.butantanBarbaro, Katia Cristina|:Pesquisador|:Lab. Imunopatologia|:pt_BR
dc.contributor.butantanDorce, Valquiria Abrão Coronado|:Pesquisador|:Lab. Farmacologia|:PrimeiroAutorpt_BR
dc.contributor.butantanNencioni, Ana Leonor Abrahão|:Pesquisador:Docente Colaborador PPGTOX|:Lab. Farmacologia|:pt_BR
dc.contributor.butantanLebrun, Ivo|:Pesquisador:Docente Permanente PPGTOX|:Lab. Bioquímica|:Autor de correspondênciapt_BR
dc.sponsorship.butantan(CAPES) Coordenação de Aperfeiçoamento de Pessoal de Nível Superior¦¦1207/2011pt_BR
dc.sponsorship.butantan(FAPESP) Fundação de Amparo à Pesquisa do Estado de São Paulo¦¦2016/10196-8pt_BR
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