Antiproliferative and antiangiogenic effect of Amblyomma sculptum (Acari: Ixodidae) crude saliva in endothelial cells in vitro

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dc.contributorLab. Genéticapt_BR
dc.contributorLab. Biologia Molecularpt_BR
dc.contributorLab. Bioquímicapt_BR
dc.contributorLab. Parasitologiapt_BR
dc.contributor.authorSá Júnior, Paulo Luiz dept_BR
dc.contributor.authorCâmara, Diana Aparecida Diaspt_BR
dc.contributor.authorSciani, Juliana Mozerpt_BR
dc.contributor.authorPorcacchia, Allan Sajpt_BR
dc.contributor.authorFonseca, Pâmela Maria Moreirapt_BR
dc.contributor.authorMendonça, Ronaldo Zucatellipt_BR
dc.contributor.authorElifio-Esposito, Selenept_BR
dc.contributor.authorSimons, Simone Michaelapt_BR
dc.identifier.citationSá Júnior PL, Câmara DAD, Sciani JM, Porcacchia AS, Fonseca PMM, Mendonça RZ, et al. Antiproliferative and antiangiogenic effect of Amblyomma sculptum (Acari: Ixodidae) crude saliva in endothelial cells in vitro. Biomed. Pharmacother.. 2019 Feb;110:353-361. doi:10.1016/j.biopha.2018.11.107.pt_BR
dc.description.abstractNeovascularization, a process that includes vasculogenesis and angiogenesis, may be a physiological or pathologic event, but in any cases the phenomenon is related to the formation of vascular net and sprouting of endothelial cells from preexisting blood vessel. The tumor environment, which counts on the tumor cell proliferation, is plenty of proangiogenic factors, such as angiogenin, TGF (a and ß), FGF, VEGF, all of them playing a crucial role in angiogenesis, an important hallmark of cancer frequently related to a poor prognosis. Therefore, therapies focusing the inhibition of cancer neovasculogenesis have become an interesting strategy for the development of antitumor therapies. In this work, we investigate the effect of tick saliva on the human endothelial cells, in order to understand its inhibitory effects on angiogenesis. To this end, the HUVEC cells were used as model of angiogenesis in vitro and the anti-proliferative, anti-migratory, cytotoxicity was evaluated. Our data depicts that saliva impairs cell development by causing structural changes while precludes cell proliferation and migration, that are crucial events related to angiogenesis. Aiming the identification of the bioactive components related to antiangiogenic activity, saliva was analyzed through the Mass Spectrometry and among all molecules identified, disintegrins and cathepsin L seems to be primarily responsible for the antiangiogenic effects of saliva.pt_BR
dc.format.extentp. 353-361pt_BR
dc.relation.ispartofBiomedicine & Pharmacotherapypt_BR
dc.rightsOpen accesspt_BR
dc.titleAntiproliferative and antiangiogenic effect of Amblyomma sculptum (Acari: Ixodidae) crude saliva in endothelial cells in vitropt_BR
dc.rights.licenseCC BY-NC-NDpt_BR
dc.contributor.externalUniversidade de Mogi das Cruzes (UMC)¦¦Brasilpt_BR
dc.contributor.externalUniversidade Federal de São Paulo (UNIFESP)¦¦Brasilpt_BR
dc.contributor.externalUniversidade de São Paulo (USP)¦¦Brasilpt_BR
dc.contributor.externalPontifícia Universidade Católica do Paraná (PUCPR)¦¦Brasilpt_BR
dc.subject.keywordAmblyomma sculptumpt_BR
dc.subject.keywordEndothelial cellspt_BR
dc.relation.ispartofabbreviatedBiomed Pharmacotherpt_BR
dc.identifier.citationabntv. 110, p. 353-361, fev. 2019pt_BR
dc.identifier.citationvancouver2019 Feb;110:353-361pt_BR
dc.contributor.butantanCâmara, Diana Aparecida Dias|:Aluno|:Lab. Genética|:pt_BR
dc.contributor.butantanSimons, Simone Michaela|:Técnico|:Lab. Parasitologia|:Autor de correspondênciapt_BR
dc.contributor.butantanSciani, Juliana Mozer|:Técnico:Docente Colaborador PPGTOX|:Lab. Biologia Molecular|:pt_BR
dc.contributor.butantanMendonça, Ronaldo Zucatelli|:Pesquisador:Docente Permanente PPGTOX|:Lab. Parasitologia|:pt_BR
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