Human recombinant Fab fragment neutralizes Shiga toxin type 2 cytotoxic effects in vitro and in vivo

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dc.contributorLab. Bacteriologiapt_BR
dc.contributor(BCA) Lab. Biofármacospt_BR
dc.contributor.authorLuz, Danielapt_BR
dc.contributor.authorAmaral, Maria Martapt_BR
dc.contributor.authorSacerdoti, Flaviapt_BR
dc.contributor.authorBernal, Alan Mauropt_BR
dc.contributor.authorQuintilio, Wagnerpt_BR
dc.contributor.authorMoro, Ana Mariapt_BR
dc.contributor.authorPalermo, Marina Sandrapt_BR
dc.contributor.authorIbarra, Cristinapt_BR
dc.contributor.authorPiazza, Roxane Maria Fontespt_BR
dc.date.accessioned2020-07-09T21:22:27Z-
dc.date.available2020-07-09T21:22:27Z-
dc.date.issued2018pt_BR
dc.identifier.citationLuz D, Amaral MM, Sacerdoti F, Bernal AM, Quintilio W, Moro AM, et al. Human recombinant Fab fragment neutralizes Shiga toxin type 2 cytotoxic effects in vitro and in vivo. Toxins. 2018 Dec;10(12):508. doi:10.3390/toxins10120508.pt_BR
dc.identifier.urihttps://repositorio.butantan.gov.br/handle/butantan/2640-
dc.description.abstractShiga toxin (Stx) producing Escherichia coli (STEC) is responsible for causing hemolytic uremic syndrome (HUS), a life-threatening thrombotic microangiopathy characterized by thrombocytopenia, hemolytic anemia, and acute renal failure after bacterially induced hemorrhagic diarrhea. Until now, there has been neither an effective treatment nor method of prevention for the deleterious effects caused by Stx intoxication. Antibodies are well recognized as affinity components of therapeutic drugs; thus, a previously obtained recombinant human FabC11:Stx2 fragment was used to neutralize Stx2 in vitro in a Vero cell viability assay. Herein, we demonstrated that this fragment neutralized, in a dose-dependent manner, the cytotoxic effects of Stx2 on human glomerular endothelial cells, on human proximal tubular epithelial cells, and prevented the morphological alterations induced by Stx2. FabC11:Stx2 protected mice from a lethal dose of Stx2 by toxin-antibody pre-incubation. Altogether, our results show the ability of a new encouraging molecule to prevent Stx-intoxication symptoms during STEC infection.pt_BR
dc.description.sponsorship(FAPESP) Fundação de Amparo à Pesquisa do Estado de São Paulopt_BR
dc.description.sponsorship(CAPES) Coordenação de Aperfeiçoamento de Pessoal de Nível Superiorpt_BR
dc.description.sponsorship(ANPCyT) Agencia Nacional de Promoción Científica y Tecnológicapt_BR
dc.description.sponsorship(CONICET) Consejo Nacional de Investigaciones Científicas y Técnicaspt_BR
dc.description.sponsorship(UBA) Universidad de Buenos Airespt_BR
dc.format.extent508pt_BR
dc.language.isoEnglishpt_BR
dc.relation.ispartofToxinspt_BR
dc.rightsOpen accesspt_BR
dc.rights.urihttps://creativecommons.org/licenses/by/4.0/pt_BR
dc.titleHuman recombinant Fab fragment neutralizes Shiga toxin type 2 cytotoxic effects in vitro and in vivopt_BR
dc.typeArticlept_BR
dc.rights.licenseCC BYpt_BR
dc.identifier.doi10.3390/toxins10120508pt_BR
dc.identifier.urlhttp://dx.doi.org/10.3390/toxins10120508pt_BR
dc.contributor.external(UBA) Universidad de Buenos Airespt_BR
dc.contributor.externalAcademia Nacional de Medicina de Buenos Airespt_BR
dc.identifier.citationvolume10pt_BR
dc.identifier.citationissue12pt_BR
dc.subject.keywordshiga toxinpt_BR
dc.subject.keywordrecombinant antibody fragmentpt_BR
dc.subject.keywordFabpt_BR
dc.subject.keywordneutralizationpt_BR
dc.subject.keywordprotectionpt_BR
dc.relation.ispartofabbreviatedToxinspt_BR
dc.identifier.citationabntv. 10, n. 12, 508, dez. 2018pt_BR
dc.identifier.citationvancouver2018 Dec;10(12):508pt_BR
dc.contributor.butantanQuintilio, Wagner|:Pesquisador|:Lab. Biofármacos |:pt_BR
dc.contributor.butantanMoro, Ana Maria|:Pesquisador|:Lab. Biofármacos |:pt_BR
dc.contributor.butantanLuz, Daniela|:Aluno|:Lab. Bacteriologia|:PrimeiroAutor:Autor de correspondênciapt_BR
dc.contributor.butantanPiazza, Roxane Maria Fontes|:Pesquisador:Docente Permanente PPGTOX|:Lab. Bacteriologia|:Autor de correspondênciapt_BR
dc.sponsorship.butantanAgencia Nacional de Promoción Científica y Tecnológica (ANPCyT)¦¦ANPCYT-PICT 2016-0292pt_BR
dc.sponsorship.butantanConsejo Nacional de Investigaciones Científicas y Técnicas (CONICET)¦¦PIP354pt_BR
dc.sponsorship.butantan(CAPES) Coordenação de Aperfeiçoamento de Pessoal de Nível Superior¦¦PNPD20132071pt_BR
dc.sponsorship.butantan(FAPESP) Fundação de Amparo à Pesquisa do Estado de São Paulo¦¦2011/12928-2pt_BR
dc.sponsorship.butantan(FAPESP) Fundação de Amparo à Pesquisa do Estado de São Paulo¦¦2017/17006-2pt_BR
dc.sponsorship.butantan(UBA) Universidad de Buenos Aires¦¦UBACYT-770pt_BR
dc.sponsorship.butantan(UBA) Universidad de Buenos Aires¦¦PDE 5-2017pt_BR
dc.identifier.bvsccBR78.1pt_BR
dc.identifier.bvsdbIBProdpt_BR
dc.description.dbindexedYespt_BR
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