An integrated analysis of mRNA and sRNA transcriptional profiles in Coffea arabica L. roots: insights on nitrogen starvation responses

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dc.contributorLaboratório Especial de Toxinologia Aplicada (LETA)pt_BR
dc.contributor.authorSantos, Tiago Benedito dospt_BR
dc.contributor.authorSoares, João D. M.pt_BR
dc.contributor.authorLima, Joni E.pt_BR
dc.contributor.authorSilva, Juliana C.pt_BR
dc.contributor.authorIvamoto, Suzana T.pt_BR
dc.contributor.authorBaba, Viviane Y.pt_BR
dc.contributor.authorSouza, Silvia G. H.pt_BR
dc.contributor.authorLorenzetti, Alan P. R.pt_BR
dc.contributor.authorPaschoal, Alexandre R.pt_BR
dc.contributor.authorMeda, Anderson R.pt_BR
dc.contributor.authorNishiyama Junior, Milton Yutakapt_BR
dc.contributor.authorOliveira, Ursula Castro dept_BR
dc.contributor.authorMokochinski, João B.pt_BR
dc.contributor.authorGuyot, Romainpt_BR
dc.contributor.authorJunqueira-de-Azevedo, Inácio de Loiola Meirellespt_BR
dc.contributor.authorFigueira, Antonio V. O.pt_BR
dc.contributor.authorMazzafera, Paulopt_BR
dc.contributor.authorJunior, Osvaldo R.pt_BR
dc.contributor.authorVieira, Luiz G. E.pt_BR
dc.contributor.authorPereira, Luiz F. P.pt_BR
dc.contributor.authorDomingues, Douglas S.pt_BR
dc.date.accessioned2020-07-09T21:22:34Z-
dc.date.available2020-07-09T21:22:34Z-
dc.date.issued2019-
dc.identifier.citationSantos TB, Soares JD.M., Lima JE., Silva JC., Ivamoto ST., Baba VY., et al. An integrated analysis of mRNA and sRNA transcriptional profiles in Coffea arabica L. roots: insights on nitrogen starvation responses. Funct Integr Genomics. 2019 Jan;19(1):151-69. doi:10.1007/s10142-018-0634-8.pt_BR
dc.identifier.urihttps://repositorio.butantan.gov.br/handle/butantan/2649-
dc.description.abstractCoffea arabica L. is an important agricultural commodity, accounting for 60% of traded coffee worldwide. Nitrogen (N) is a macronutrient that is usually limiting to plant yield; however, molecular mechanisms of plant acclimation to N limitation remain largely unknown in tropical woody crops. In this study, we investigated the transcriptome of coffee roots under N starvation, analyzing poly-A+ libraries and small RNAs. We also evaluated the concentration of selected amino acids and N-source preferences in roots. Ammonium was preferentially taken up over nitrate, and asparagine and glutamate were the most abundant amino acids observed in coffee roots. We obtained 34,654 assembled contigs by mRNA sequencing, and validated the transcriptional profile of 12 genes by RT-qPCR. Illumina small RNA sequencing yielded 8,524,332 non-redundant reads, resulting in the identification of 86 microRNA families targeting 253 genes. The transcriptional pattern of eight miRNA families was also validated. To our knowledge, this is the first catalog of differentially regulated amino acids, N sources, mRNAs, and sRNAs in Arabica coffee roots.pt_BR
dc.description.sponsorshipConsórcio Pesquisa Cafépt_BR
dc.description.sponsorshipFundação Araucária de Apoio ao Desenvolvimento Científico e Tecnológico do Estado do Paraná (FA)pt_BR
dc.description.sponsorshipCoordenação de Aperfeiçoamento de Pessoal de Nível Superior (CAPES)pt_BR
dc.description.sponsorshipConselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq)pt_BR
dc.description.sponsorshipFundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)pt_BR
dc.format.extentp. 151-169pt_BR
dc.languageengpt_BR
dc.relation.ispartofFunctional & Integrative Genomicspt_BR
dc.titleAn integrated analysis of mRNA and sRNA transcriptional profiles in Coffea arabica L. rootspt_BR
dc.typeArticlept_BR
dc.identifier.doihttps://repositorio.butantan.gov.br/handle/butantan/2649pt_BR
dc.identifier.urlhttp://dx.doi.org/10.1007/s10142-018-0634-8pt_BR
dc.contributor.externalInstituto Agronômico do Paraná (IAPAR)¦¦Brasilpt_BR
dc.contributor.externalUniversidade do Oeste Paulista (UNOESTE)¦¦Brasilpt_BR
dc.contributor.externalUniversidade de São Paulo (USP)¦¦Brasilpt_BR
dc.contributor.externalUniversidade Federal de Minas Gerais (UFMG)¦¦Brasilpt_BR
dc.contributor.externalUniversidade Tecnológica Federal do Paraná (UTFPR)¦¦Brasilpt_BR
dc.contributor.externalUniversidade Estadual Paulista Júlio de Mesquita Filho (UNESP)¦¦Brasilpt_BR
dc.contributor.externalUniversidade Paranaense (UNIPAR)¦¦Brasilpt_BR
dc.contributor.externalUniversidade Estadual de Londrina (UEL)¦¦Brasilpt_BR
dc.contributor.externalUniversidade Estadual de Campinas (UNICAMP)¦¦Brasilpt_BR
dc.contributor.externalInstitut de Recherche pour le Développement (IRD)¦¦Françapt_BR
dc.contributor.externalEmbrapa Caf馦Brasilpt_BR
dc.title.subinsights on nitrogen starvation responsespt_BR
dc.identifier.citationvolume19pt_BR
dc.identifier.citationissue1pt_BR
dc.subject.keywordCoffeept_BR
dc.subject.keywordNitrogen transportpt_BR
dc.subject.keywordDifferential gene expressionpt_BR
dc.subject.keywordRNA-seqpt_BR
dc.subject.keywordmicroRNApt_BR
dc.relation.ispartofabbreviatedFunct Integr Genomicspt_BR
dc.identifier.citationabntv. 19, n. 1, p.151-169, jan. 2019pt_BR
dc.identifier.citationvancouver2019 Jan;19(1):151-69pt_BR
dc.contributor.butantanNishiyama Junior, Milton Yutaka|:Pesquisador|:Laboratório Especial de Toxinologia Aplicada (LETA)|:pt_BR
dc.contributor.butantanOliveira, Ursula Castro de|:Aluno|:Laboratório Especial de Toxinologia Aplicada (LETA)|:pt_BR
dc.contributor.butantanJunqueira-de-Azevedo, Inácio de Loiola Meirelles|:Pesquisador:Docente Permanente PPGTOX|:Laboratório Especial de Toxinologia Aplicada (LETA)|:pt_BR
dc.sponsorship.butantanConselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq)¦¦pt_BR
dc.sponsorship.butantanConsórcio Pesquisa Caf馦pt_BR
dc.sponsorship.butantanCoordenação de Aperfeiçoamento de Pessoal de Nível Superior (CAPES)¦¦PVE 084/2013pt_BR
dc.sponsorship.butantanFundação Araucária de Apoio ao Desenvolvimento Científico e Tecnológico do Estado do Paraná (FA)¦¦pt_BR
dc.sponsorship.butantanFundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)¦¦pt_BR
dc.identifier.bvsccBR78.1pt_BR
dc.identifier.bvsdbIBProdpt_BR
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