Phagocytosis of Leptospira by leukocytes from mice with different susceptibility to leptospirosis and possible role of chemokines

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dc.contributorLab. Bacteriologiapt_BR
dc.contributorLab. Imunogenéticapt_BR
dc.contributorLab. Desenvolvimento de Processospt_BR
dc.contributorCentro Bioindustrialpt_BR
dc.contributor.authorSilva, Paloma Luiza Duarte dapt_BR
dc.contributor.authorFerreira, Fabiana Laurettipt_BR
dc.contributor.authorLima, Maiara Caldas dept_BR
dc.contributor.authorLima, Swiany Silveirapt_BR
dc.contributor.authorCovarrubias, Ambart E.pt_BR
dc.contributor.authorDe Franco, Marcelopt_BR
dc.contributor.authorCarvalho, Eneaspt_BR
dc.contributor.authorHo, Paulo Leept_BR
dc.contributor.authorCosta, Renata Maria Augusto dapt_BR
dc.contributor.authorMartins, Elizabeth Angelica Lemept_BR
dc.contributor.authorSilva, Josefa Bezerra dapt_BR
dc.identifier.citationSilva PLD, Ferreira FL, Lima MC, Lima SS, Covarrubias AE., De Franco M, et al. Phagocytosis of Leptospira by leukocytes from mice with different susceptibility to leptospirosis and possible role of chemokines. BMC Microbiol. 2019;19:4. doi:10.1186/s12866-018-1371-9.pt_BR
dc.description.abstractBackground: Leptospirosis is a widespread zoonosis caused by pathogenic prokaryotic microbes of the genus Leptospira. Although there are several reports in the literature, host-pathogen interaction is still poorly understood. The role of chemokine expression is important on the chemotaxis, activation and regulation of immune cells. Recent studies have shown that their expression profiles play an important role on the severity of leptospirosis outcome. We evaluated the phagocytosis of Leptospira by spleens cells from C3H/HeJ, C3H/HePas and BALB/c mouse strains, respectively susceptible, intermediate and resistant to leptospirosis, and by RAW 264.7 macrophages. Besides, we evaluated the effects of CCL2 treatment on the phagocytosis. The cells were incubated with or without CCL2 chemokine, and infected with virulent L. interrogans sv Copenhageni. Cells and culture supernatants were collected for subsequent analysis. Results: The number of leptospires was higher in BALB/c cells, CCL2 pre-treated or only infected groups, when compared to C3H/HeJ and C3H/HePas cells. Indeed, CCL2 activation did not interfere in the phagocytosis of Leptospira. Expression of chemokines CXCL5 and CCL8 levels were significantly inhibited in infected BALB/c cells when compared to the non-infected control. Conclusions: Higher ability to phagocytosis and early modulation of some chemokines correlated with the resistance to leptospirosis disease. Exposure to CCL2 did not interfere on phagocytosis of Leptospira in our experimental conditions, but acted in the modulation of chemokines expression during Leptospira infection.pt_BR
dc.description.sponsorshipFundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)pt_BR
dc.description.sponsorshipConselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq)pt_BR
dc.relation.ispartofBMC Microbiologypt_BR
dc.rightsOpen Accesspt_BR
dc.titlePhagocytosis of Leptospira by leukocytes from mice with different susceptibility to leptospirosis and possible role of chemokinespt_BR
dc.contributor.externalFundação Ezequiel Dias (FUNED)¦¦Brasilpt_BR
dc.contributor.externalUniversidad San Sebastián¦¦Chilept_BR
dc.contributor.externalInstituto Pasteur¦¦Brasilpt_BR
dc.relation.ispartofabbreviatedBMC Microbiolpt_BR
dc.identifier.citationabntv. 19, 4, 2019pt_BR
dc.contributor.butantanSilva, Paloma Luiza Duarte da|:Aluno|:Lab. Bacteriologia|:PrimeiroAutorpt_BR
dc.contributor.butantanLauretti Ferreira, Fabiana|:Aluno|:Lab. Bacteriologia|:pt_BR
dc.contributor.butantanLima, Maiara Caldas de|:|:Lab. Bacteriologia|:pt_BR
dc.contributor.butantanDe Franco, Marcelo|:Pesquisador|:Lab. Imunogenética|:pt_BR
dc.contributor.butantanCarvalho, Eneas|:Pesquisador|:Lab. Bacteriologia|:pt_BR
dc.contributor.butantanHo, Paulo Lee|:Pesquisador|:Centro Bioindustrial|:pt_BR
dc.contributor.butantanCosta, Renata Maria Augusto da|:Aluno|:Lab. Bacteriologia|:pt_BR
dc.contributor.butantanMartins, Elizabeth Angelica Leme|:Pesquisador|:Lab. Desenvolvimento de Processos|:pt_BR
dc.contributor.butantanSilva, Josefa Bezerra da|:Pesquisador|:Lab. Bacteriologia|:Autor de correspondênciapt_BR
dc.sponsorship.butantanConselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq)¦¦473035/2013-8pt_BR
dc.sponsorship.butantanFundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)¦¦2013/16451-1pt_BR
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