Amniotic membrane as a biological scaffold for dental pulp stem cell transplantation in ocular surface reconstruction

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dc.contributorLab. Genéticapt_BR
dc.contributor.authorMonteiro, Bábyla Geraldespt_BR
dc.contributor.authorLoureiro, Renata Ruocopt_BR
dc.contributor.authorCristovam, Priscila Cardosopt_BR
dc.contributor.authorCovre, Joyce Lucianapt_BR
dc.contributor.authorPereira Gomes, José Alvaropt_BR
dc.contributor.authorKerkis, Irinapt_BR
dc.identifier.citationMonteiro BG, Loureiro RR, Cristovam PC, Covre JL, Pereira Gomes JA, Kerkis I. Amniotic membrane as a biological scaffold for dental pulp stem cell transplantation in ocular surface reconstruction. Arq Bras Oftalmol. 2019 Jan-Feb;82(1):32-37. doi:10.5935/0004-2749.20190009.pt_BR
dc.description.abstractPurpose: To evaluate the ability of human immature dental pulp stem cells, which are mesenchymal stem cells of neural crest origin, to differentiate into the corneal epithelium for purposes of corneal transplantation and tissue engineering when cultured on de-epithelized amniotic membranes. Methods: We compared the immunophenotypes (ABCG2, K3/12, and vimentin) of cells grown on amniotic membranes or plastic surfaces under serum-free conditions or in culture media containing serum or serum replacement components. Results: Immature dental pulp stem cells grown on amniotic membranes under basal conditions are able to maintain their undifferentiated state. Our data also suggest that the culture medium used in the present work can modulate the expression of immature dental pulp stem cell markers, thus inducing epithelial differentiation of these cells in vitro. Conclusions: Our results suggest that the amniotic membrane is a good choice for the growth and transplantation of mesenchymal stem cells, particularly immature dental pulp stem cells, in clinical ocular surface reconstruction.pt_BR
dc.description.sponsorshipFundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)pt_BR
dc.format.extentp. 32-37pt_BR
dc.relation.ispartofArquivos Brasileiros de Oftalmologiapt_BR
dc.rightsOpen Accesspt_BR
dc.titleAmniotic membrane as a biological scaffold for dental pulp stem cell transplantation in ocular surface reconstructionpt_BR
dc.contributor.externalUniversidade Federal de São Paulo (UNIFESP)¦¦Brasilpt_BR
dc.subject.keywordCorneal epitheliapt_BR
dc.subject.keywordLimbus cornaept_BR
dc.subject.keywordStem cellpt_BR
dc.subject.keywordDental pulppt_BR
dc.subject.keywordAmniotic membranept_BR
dc.subject.keywordOcular surfacept_BR
dc.relation.ispartofabbreviatedArq Bras Oftalmolpt_BR
dc.identifier.citationabntv. 82, n. 1, p. 32-37, jan./fev. 2019pt_BR
dc.identifier.citationvancouver2019 Jan-Feb;82(1):32-37pt_BR
dc.contributor.butantanMonteiro, Bábyla Geraldes|:Aluno|:Lab. Genética|:PrimeiroAutorpt_BR
dc.contributor.butantanKerkis, Irina|:Pesquisador:Docente Permanente PPGTOX|:Lab. Genética|:Autor de correspondênciapt_BR
dc.sponsorship.butantanFundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)¦¦2010/08595-5pt_BR
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