Naja annulifera snake: new insights into the venom components and pathogenesis of envenomation

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dc.contributorLab. Imunoquímicapt_BR
dc.contributorLaboratório de Toxinologia Aplicadapt_BR
dc.contributor.authorFrança, Felipe Silva dept_BR
dc.contributor.authorVillas-Boas, Isadora Mariapt_BR
dc.contributor.authorSerrano, Solange Maria de Toledopt_BR
dc.contributor.authorCogliati, Brunopt_BR
dc.contributor.authorChudzinski, Sonia Aparecida de Andradept_BR
dc.contributor.authorLopes, Priscila Hesspt_BR
dc.contributor.authorKitano, Eduardo Shigueopt_BR
dc.contributor.authorOkamoto, Cinthya Kimoript_BR
dc.contributor.authorTambourgi, Denise Vilarinhopt_BR
dc.identifier.citationFrança FS, Villas-Boas IM, Serrano SMT, Cogliati B, Chudzinski SAA, Lopes PH, et al. Naja annulifera snake: new insights into the venom components and pathogenesis of envenomation. Plos Neglect. Trop. Dis.. 2019 Jan;13(1):e0007017. doi:10.1371/journal.pntd.0007017.pt_BR
dc.description.abstractBackground Naja annulifera is a medically important venomous snake occurring in some of the countries in Sub-Saharan Africa. Accidental bites result in severe coagulation disturbances, systemic inflammation and heart damage, as reported in dogs, and death, by respiratory arrest, in humans. Despite the medical importance of N. annulifera, little is known about its venom composition and the pathogenesis of envenomation. In this paper, the toxic, inflammatory and immunogenic properties of N. annulifera venom were analyzed. Methodology/Principal findings Venom proteomic analysis identified 79 different proteins, including Three Finger Toxins, Cysteine Rich Secretory Proteins, Metalloproteinases, Phospholipases A2 (PLA2), Hyaluronidase, L-amino-acid oxidase, Cobra Venom Factor and Serine Proteinase. The presence of PLA2, hyaluronidase, fibrinogenolytic and anticoagulant activities was detected using functional assays. The venom was cytotoxic to human keratinocytes. In an experimental murine model of envenomation, it was found that the venom induced local changes, such as swelling, which was controlled by anti-inflammatory drugs. Moreover, the venom caused death, which was preceded by systemic inflammation and pulmonary hemorrhage. The venom was shown to be immunogenic, inducing a strong humoral immune response, with the production of antibodies able to recognize venom components with high molecular weight and to neutralize its lethal activity. Conclusions/Significance The results obtained in this study demonstrate that N. annulifera venom contains toxins able to induce local and systemic inflammation, which can contribute to lung damage and death. Moreover, the venom is immunogenic, an important feature that must be considered during the production of a therapeutic anti-N. annulifera antivenom.pt_BR
dc.description.sponsorship(FAPESP) Fundação de Amparo à Pesquisa do Estado de São Paulopt_BR
dc.description.sponsorship(CNPq) Conselho Nacional de Desenvolvimento Científico e Tecnológicopt_BR
dc.description.sponsorship(CAPES) Coordenação de Aperfeiçoamento de Pessoal de Nível Superiorpt_BR
dc.relation.ispartofPlos Neglected Tropical Diseasespt_BR
dc.rightsOpen accesspt_BR
dc.titleNaja annulifera snake: new insights into the venom components and pathogenesis of envenomationpt_BR
dc.rights.licenseCC BYpt_BR
dc.contributor.external(USP) Universidade de São Paulopt_BR
dc.relation.ispartofabbreviatedPlos Neglect Trop Dispt_BR
dc.identifier.citationabntv. 13, n. 1, e0007017, jan. 2019pt_BR
dc.identifier.citationvancouver2019 Jan;13(1):e0007017pt_BR
dc.contributor.butantanFrança, Felipe Silva de|:Aluno|:Lab. Imunoquímica|:PrimeiroAutorpt_BR
dc.contributor.butantanSerrano, Solange Maria de Toledo|:Pesquisador:Docente Permanente PPGTOX|:Laboratório de Toxinologia Aplicada|:pt_BR
dc.contributor.butantanChudzinski, Sonia Aparecida de Andrade|:Pesquisador:Docente Colaborador PPGTOX|:Laboratório de Toxinologia Aplicada|:pt_BR
dc.contributor.butantanTambourgi, Denise Vilarinho|:Pesquisador:Docente Permanente PPGTOX|:Lab. Imunoquímica|:Autor de correspondênciapt_BR
dc.contributor.butantanVillas-Boas, Isadora Maria|:Aluno|:Lab. Imunoquímica|:pt_BR
dc.contributor.butantanLopes, Priscila Hess|:Aluno|:Lab. Imunoquímica|:pt_BR
dc.contributor.butantanKitano, Eduardo Shigueo|:Aluno|:Laboratório de Toxinologia Aplicada|:pt_BR
dc.contributor.butantanOkamoto, Cinthya Kimori|:Técnico|:Lab. Imunoquímica|:pt_BR
dc.sponsorship.butantan(CNPq) Conselho Nacional de Desenvolvimento Científico e Tecnológico¦¦301358/2017-6pt_BR
dc.sponsorship.butantan(CAPES) Coordenação de Aperfeiçoamento de Pessoal de Nível Superior¦¦pt_BR
dc.sponsorship.butantan(FAPESP) Fundação de Amparo à Pesquisa do Estado de São Paulo¦¦2013/07467-1pt_BR
dc.subject.researchlineToxinas e sistemas biológicospt_BR
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