Amblyomma sculptum salivary PGE2 modulates the dendritic cell-Rickettsia rickettsii interactions in vitro and in vivo

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dc.contributorLaboratório de Toxinologia Aplicadapt_BR
dc.contributor.authorEsteves, Elianept_BR
dc.contributor.authorBizzarro, Brunapt_BR
dc.contributor.authorCosta, Francisco Borgespt_BR
dc.contributor.authorRamírez-Hernández, Alejandropt_BR
dc.contributor.authorPeti, Ana Paula Ferrantipt_BR
dc.contributor.authorCataneo, Allan Henrique Depieript_BR
dc.contributor.authorWowk, Pryscilla Faninipt_BR
dc.contributor.authorTimóteo, Rodolfo Pessatopt_BR
dc.contributor.authorLabruna, Marcelo Bahiapt_BR
dc.contributor.authorSilva Junior, Pedro Ismael dapt_BR
dc.contributor.authorSilva, Célio Lopespt_BR
dc.contributor.authorFaccioli, Lúcia Helenapt_BR
dc.contributor.authorFogaça, Andréa Cristinapt_BR
dc.contributor.authorSorgi, Carlos Arteriopt_BR
dc.contributor.authorSá-Nunes, Andersonpt_BR
dc.identifier.citationEsteves E, Bizzarro B, Costa FB, Ramírez-Hernández A, Peti APF, Cataneo AHD, et al. Amblyomma sculptum salivary PGE2 modulates the dendritic cell-Rickettsia rickettsii interactions in vitro and in vivo. Front. Immunol.. 2019 Feb;10:118. doi:10.3389/fimmu.2019.00118.pt_BR
dc.description.abstractAmblyomma sculptum is an important vector of Rickettsia rickettsii, causative agent of Rocky Mountain spotted fever and the most lethal tick-borne pathogen affecting humans. To feed on the vertebrate host’s blood, A. sculptum secretes a salivary mixture, which may interact with skin resident dendritic cells (DCs) and modulate their function. The present work was aimed at depicting the A. sculptum saliva-host DC network and the biochemical nature of the immunomodulatory component(s) involved in this interface. A. sculptum saliva inhibits the production of inflammatory cytokines by murine DCs stimulated with LPS. The fractionation of the low molecular weight salivary content by reversed-phase chromatography revealed active fractions eluting from 49 to 55% of the acetonitrile gradient. Previous studies suggested that this pattern of elution matches with that observed for prostaglandin E2 (PGE2) and the molecular identity of this lipid mediator was unambiguously confirmed by a new high-resolution mass spectrometry methodology. A productive infection of murine DCs by R. rickettsii was demonstrated for the first time leading to proinflammatory cytokine production that was inhibited by both A. sculptum saliva and PGE2, a result also achieved with human DCs. The adoptive transfer of murine DCs incubated with R. rickettsii followed by treatment with A. sculptum saliva or PGE2 did not change the cytokine profile associated to cellular recall responses while IgG2a-specific antibodies were decreased in the serum of these mice. Together, these findings emphasize the role of PGE2 as a universal immunomodulator of tick saliva. In addition, it contributes to new approaches to explore R. rickettsii-DC interactions both in vitro and in vivo.pt_BR
dc.relation.ispartofFrontiers in Immunologypt_BR
dc.rightsOpen accesspt_BR
dc.titleAmblyomma sculptum salivary PGE2 modulates the dendritic cell-Rickettsia rickettsii interactions in vitro and in vivopt_BR
dc.rights.licenseCC BYpt_BR
dc.contributor.external(USP) Universidade de São Paulopt_BR
dc.contributor.external(FIOCRUZ) Fundação Oswaldo Cruzpt_BR
dc.contributor.externalUniversidade Federal do Triângulo Mineiro (UFTM)pt_BR
dc.contributor.external(INCT - EM) Instituto Nacional de Ciência e Tecnologia em Entomologia Molecularpt_BR
dc.subject.keywordAmblyomma sculptumpt_BR
dc.subject.keywordtick salivapt_BR
dc.subject.keyworddendritic cellspt_BR
dc.subject.keywordRickettsia rickettsiipt_BR
dc.relation.ispartofabbreviatedFront Immunolpt_BR
dc.identifier.citationabntv. 10, 118, fev. 2019pt_BR
dc.identifier.citationvancouver2019 Feb;10:118pt_BR
dc.contributor.butantanSilva Junior, Pedro Ismael da|:Pesquisador|:Laboratório de Toxinologia Aplicada|:pt_BR
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