Ortholog of the polymerase theta helicase domain modulates DNA replication in Trypanosoma cruzi

Full metadata record
DC FieldValueLanguage
dc.contributorLCC - Laboratório de Ciclo Celularpt_BR
dc.contributorCentro de Toxinas, Resposta-imune e Sinalização Celular (CeTICS)pt_BR
dc.contributorLab. Parasitologiapt_BR
dc.contributorLaboratório de Toxinologia Aplicadapt_BR
dc.contributor.authorLima, Loyze Paola Oliveira dept_BR
dc.contributor.authorCalderano, Simone Guedespt_BR
dc.contributor.authorSilva, Marcelo Santos dapt_BR
dc.contributor.authorde Araujo, Christiane Bezerrapt_BR
dc.contributor.authorVasconcelos, Elton José Rosas dept_BR
dc.contributor.authorIwai, Leo Keipt_BR
dc.contributor.authorPereira, Cláudio A.pt_BR
dc.contributor.authorFragoso, Stenio P.pt_BR
dc.contributor.authorElias, Maria Carolinapt_BR
dc.date.accessioned2020-07-09T21:23:00Z-
dc.date.available2020-07-09T21:23:00Z-
dc.date.issued2019pt_BR
dc.identifier.citationLima LPO, Calderano SG, Silva MS, de Araujo CB, Vasconcelos EJR, Iwai LK, et al. Ortholog of the polymerase theta helicase domain modulates DNA replication in Trypanosoma cruzi. Sci. Rep.. 2019 Feb;9:2888. doi:10.1038/s41598-019-39348-2.pt_BR
dc.identifier.urihttps://repositorio.butantan.gov.br/handle/butantan/2679-
dc.description.abstractDNA polymerase theta (Pol theta), a member of the DNA polymerase family A, exhibits a polymerase C-terminal domain, a central domain, and an N-terminal helicase domain. Pol theta plays important roles in DNA repair via its polymerase domain, regulating genome integrity. In addition, in mammals, Pol theta modulates origin firing timing and MCM helicase recruitment to chromatin. In contrast, as a model eukaryote, Trypanosoma cruzi exhibits two individual putative orthologs of Pol theta in different genomic loci; one ortholog is homologous to the Pol theta C-terminal polymerase domain, and the other is homologous to the Pol theta helicase domain, called Pol theta-polymerase and Pol theta-helicase, respectively. A pull-down assay using the T. cruzi component of the prereplication complex Orc1/Cdc6 as bait captured Pol theta-helicase from the nuclear extract. Orc1/Cdc6 and Pol theta-helicase directly interacted, and Pol theta-helicase presented DNA unwinding and ATPase activities. A T. cruzi strain overexpressing the Pol theta-helicase domain exhibited a significantly decreased amount of DNA-bound MCM7 and impaired replication origin firing. Taken together, these data suggest that Pol theta-helicase modulates DNA replication by directly interacting with Orc1/Cdc6, which reduces the binding of MCM7 to DNA and thereby impairs the firing of replication origins.pt_BR
dc.description.sponsorship(FAPESP) Fundação de Amparo à Pesquisa do Estado de São Paulopt_BR
dc.description.sponsorship(CNPq) Conselho Nacional de Desenvolvimento Científico e Tecnológicopt_BR
dc.format.extent2888pt_BR
dc.language.isoEnglishpt_BR
dc.relation.ispartofScientific Reportspt_BR
dc.rightsOpen accesspt_BR
dc.rights.urihttps://creativecommons.org/licenses/by/4.0/pt_BR
dc.titleOrtholog of the polymerase theta helicase domain modulates DNA replication in Trypanosoma cruzipt_BR
dc.typeArticlept_BR
dc.rights.licenseCC BYpt_BR
dc.identifier.doi10.1038/s41598-019-39348-2pt_BR
dc.identifier.urlhttps://doi.org/10.1038/s41598-019-39348-2pt_BR
dc.contributor.externalWestern University of Health Sciences (WesternU)¦¦Estados Unidospt_BR
dc.contributor.externalUniversidad de Buenos Aires (UBA)¦¦Argentinapt_BR
dc.contributor.externalFundação Oswaldo Cruz (Fiocruz)¦¦Brasilpt_BR
dc.identifier.citationvolume9pt_BR
dc.relation.ispartofabbreviatedSci Reppt_BR
dc.identifier.citationabntv. 9, 2888, fev. 2019pt_BR
dc.identifier.citationvancouver2019 Feb;9:2888pt_BR
dc.contributor.butantanLima, Loyze Paola Oliveira de|:Aluno|:LCC - Laboratório de Ciclo Celular|:PrimeiroAutorpt_BR
dc.contributor.butantanSilva, Marcelo Santos da|:Aluno|:LCC - Laboratório de Ciclo Celular:Centro de Toxinas, Resposta-imune e Sinalização Celular (CeTICS)|:pt_BR
dc.contributor.butantanDe Araujo, Christiane Bezerra|:Aluno|:LCC - Laboratório de Ciclo Celular:Centro de Toxinas, Resposta-imune e Sinalização Celular (CeTICS)|:pt_BR
dc.contributor.butantanElias, Maria Carolina|:Pesquisador|:LCC - Laboratório de Ciclo Celular:Centro de Toxinas, Resposta-imune e Sinalização Celular (CeTICS)|:Autor de correspondênciapt_BR
dc.contributor.butantanIwai, Leo Kei|:Pesquisador:Docente Colaborador PPGTOX|:Laboratório de Toxinologia Aplicada|:pt_BR
dc.contributor.butantanCalderano, Simone Guedes|:Pesquisador|:Lab. Parasitologia|:pt_BR
dc.sponsorship.butantanConselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq)¦¦870219/1997-9pt_BR
dc.sponsorship.butantanConselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq)¦¦304329/2015-0pt_BR
dc.sponsorship.butantanConselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq)¦¦140218/2017-3pt_BR
dc.sponsorship.butantanFundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)¦¦2013/07467-1pt_BR
dc.sponsorship.butantanFundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)¦¦2016/50050-2pt_BR
dc.sponsorship.butantanFundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)¦¦2014/24170-5pt_BR
dc.sponsorship.butantanFundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)¦¦2014/13375-5pt_BR
dc.sponsorship.butantanFundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)¦¦2015-10037-4pt_BR
dc.identifier.bvsccBR78.1pt_BR
dc.identifier.bvsdbIBProdpt_BR
dc.description.dbindexedYespt_BR
item.fulltextCom Texto completo-
item.grantfulltextopen-
item.languageiso639-1English-
item.openairetypeArticle-
crisitem.author.dept#PLACEHOLDER_PARENT_METADATA_VALUE#-
crisitem.author.dept#PLACEHOLDER_PARENT_METADATA_VALUE#-
crisitem.author.dept#PLACEHOLDER_PARENT_METADATA_VALUE#-
crisitem.author.dept#PLACEHOLDER_PARENT_METADATA_VALUE#-
crisitem.author.dept#PLACEHOLDER_PARENT_METADATA_VALUE#-
crisitem.author.dept#PLACEHOLDER_PARENT_METADATA_VALUE#-
crisitem.author.dept#PLACEHOLDER_PARENT_METADATA_VALUE#-
crisitem.author.dept#PLACEHOLDER_PARENT_METADATA_VALUE#-
crisitem.author.dept#PLACEHOLDER_PARENT_METADATA_VALUE#-
crisitem.author.orcid#PLACEHOLDER_PARENT_METADATA_VALUE#-
crisitem.author.orcid0000-0003-1331-6875-
crisitem.author.orcid#PLACEHOLDER_PARENT_METADATA_VALUE#-
crisitem.author.orcid#PLACEHOLDER_PARENT_METADATA_VALUE#-
crisitem.author.orcid0000-0001-5130-6622-
crisitem.author.orcid0000-0002-1571-7763-
crisitem.author.orcid#PLACEHOLDER_PARENT_METADATA_VALUE#-
crisitem.author.orcid#PLACEHOLDER_PARENT_METADATA_VALUE#-
crisitem.author.orcid#PLACEHOLDER_PARENT_METADATA_VALUE#-
crisitem.author.parentorg#PLACEHOLDER_PARENT_METADATA_VALUE#-
crisitem.author.parentorg#PLACEHOLDER_PARENT_METADATA_VALUE#-
crisitem.journal.journalissn#PLACEHOLDER_PARENT_METADATA_VALUE#-
crisitem.journal.journaleissn#PLACEHOLDER_PARENT_METADATA_VALUE#-
Appears in Collections:Artigos de periódicos


Files in This Item:

10.1038s41598-019-39348-2.pdf
Size: 3.45 MB
Format: Adobe PDF
View/Open
Show simple item record

This item is licensed under a Creative Commons License Creative Commons