Antitumoral effects of Amblyomma sculptum Berlese saliva in neuroblastoma cell lines involve cytoskeletal deconstruction and cell cycle arrest

Full metadata record
DC FieldValueLanguage
dc.contributorLab. Genéticapt_BR
dc.contributorLab. Parasitologiapt_BR
dc.contributor.authorNascimento, Thatyanne Gradowski dopt_BR
dc.contributor.authorVieira, Priscilla Santospt_BR
dc.contributor.authorCogo, Sheron Campospt_BR
dc.contributor.authorDias-Netipanyj, Marcela Ferreirapt_BR
dc.contributor.authorFrança Junior, Nilton dept_BR
dc.contributor.authorCâmara, Diana Aparecida Diaspt_BR
dc.contributor.authorPorcacchia, Allan Sajpt_BR
dc.contributor.authorMendonça, Ronaldo Zucatellipt_BR
dc.contributor.authorMoreno-Amaral, Andréa Novaispt_BR
dc.contributor.authorSá Júnior, Paulo Luiz dept_BR
dc.contributor.authorSimons, Simone Michaelapt_BR
dc.contributor.authorZischler, Lucianapt_BR
dc.contributor.authorElifio-Esposito, Selenept_BR
dc.date.accessioned2020-07-09T21:23:28Z-
dc.date.available2020-07-09T21:23:28Z-
dc.date.issued2019pt_BR
dc.identifier.citationNascimento TG, Vieira PS, Cogo SC, Dias-Netipanyj MF, França Junior N, Câmara DAD, et al. Antitumoral effects of Amblyomma sculptum Berlese saliva in neuroblastoma cell lines involve cytoskeletal deconstruction and cell cycle arrest. Rev Bras Parasitol Vet. 2019 Jan/Mar;28(1):126-133. doi:10.1590/s1984-296120180098.pt_BR
dc.identifier.urihttps://repositorio.butantan.gov.br/handle/butantan/2710-
dc.description.abstractThe antitumor properties of ticks salivary gland extracts or recombinant proteins have been reported recently, but little is known about the antitumor properties of the secreted components of saliva. The goal of this study was to investigate the in vitro effect of the saliva of the hard tick Amblyomma sculptum on neuroblastoma cell lines. SK-N-SK, SH-SY5Y, Be(2)-M17, IMR-32, and CHLA-20 cells were susceptible to saliva, with 80% reduction in their viability compared to untreated controls, as demonstrated by the methylene blue assay. Further investigation using CHLA-20 revealed apoptosis, with approximately 30% of annexin-V positive cells, and G0/G1-phase accumulation (>60%) after treatment with saliva. Mitochondrial membrane potential (??m) was slightly, but significantly (p < 0.05), reduced and the actin cytoskeleton was disarranged, as indicated by fluorescent microscopy. The viability of human fibroblast (HFF-1 cells) used as a non-tumoral control decreased by approximately 40%. However, no alterations in cell cycle progression, morphology, and ??m were observed in these cells. The present work provides new perspectives for the characterization of the molecules present in saliva and their antitumor properties.pt_BR
dc.description.sponsorship(CAPES) Coordenação de Aperfeiçoamento de Pessoal de Nível Superiorpt_BR
dc.description.sponsorship(FA) Fundação Araucária de Apoio ao Desenvolvimento Científico e Tecnológico do Estado do Paranápt_BR
dc.format.extentp. 126-133pt_BR
dc.language.isoEnglishpt_BR
dc.relation.ispartofBrazilian Journal of Veterinary Parasitologypt_BR
dc.rightsOpen accesspt_BR
dc.rights.urihttps://creativecommons.org/licenses/by/4.0/pt_BR
dc.titleAntitumoral effects of Amblyomma sculptum Berlese saliva in neuroblastoma cell lines involve cytoskeletal deconstruction and cell cycle arrestpt_BR
dc.typeArticlept_BR
dc.rights.licenseCC BYpt_BR
dc.identifier.doi10.1590/s1984-296120180098pt_BR
dc.identifier.urlhttp://dx.doi.org/10.1590/s1984-296120180098pt_BR
dc.contributor.externalPontifícia Universidade Católica do Paraná (PUCPR)¦¦Brasilpt_BR
dc.identifier.citationvolume28pt_BR
dc.identifier.citationissue1pt_BR
dc.subject.keywordtick salivapt_BR
dc.subject.keywordanimal toxinpt_BR
dc.subject.keywordtumor cell deathpt_BR
dc.subject.keywordpediatric cancerpt_BR
dc.relation.ispartofabbreviatedRev Bras Parasitol Vetpt_BR
dc.identifier.citationabntv. 28, n. 1, p. 126-133, jan.-mar. 2019pt_BR
dc.identifier.citationvancouver2019 Jan/Mar;28(1):126-133pt_BR
dc.contributor.butantanCâmara, Diana Aparecida Dias|:Aluno|:Lab. Genética|:pt_BR
dc.contributor.butantanMendonça, Ronaldo Zucatelli|:Pesquisador:Docente Permanente PPGTOX|:Lab. Parasitologia|:pt_BR
dc.contributor.butantanPorcacchia, Allan Saj|:Aluno|:Lab. Genética|:pt_BR
dc.contributor.butantanSá Júnior, Paulo Luiz de|:Aluno|:Lab. Genética|:pt_BR
dc.contributor.butantanSimons, Simone Michaela|:Técnico|:Lab. Parasitologia|:pt_BR
dc.sponsorship.butantan(CAPES) Coordenação de Aperfeiçoamento de Pessoal de Nível Superior¦¦16/2015 #880/2017, 001pt_BR
dc.sponsorship.butantanFundação Araucária de Apoio ao Desenvolvimento Científico e Tecnológico do Estado do Paraná (FA)¦¦09/2016pt_BR
dc.identifier.bvsccBR78.1pt_BR
dc.identifier.bvsdbIBProdpt_BR
dc.description.dbindexedYespt_BR
item.fulltextCom Texto completo-
item.languageiso639-1English-
item.grantfulltextopen-
item.openairetypeArticle-
crisitem.journal.journalissn#PLACEHOLDER_PARENT_METADATA_VALUE#-
crisitem.journal.journaleissn#PLACEHOLDER_PARENT_METADATA_VALUE#-
crisitem.author.dept#PLACEHOLDER_PARENT_METADATA_VALUE#-
crisitem.author.dept#PLACEHOLDER_PARENT_METADATA_VALUE#-
crisitem.author.dept#PLACEHOLDER_PARENT_METADATA_VALUE#-
crisitem.author.dept#PLACEHOLDER_PARENT_METADATA_VALUE#-
crisitem.author.dept#PLACEHOLDER_PARENT_METADATA_VALUE#-
crisitem.author.dept#PLACEHOLDER_PARENT_METADATA_VALUE#-
crisitem.author.dept#PLACEHOLDER_PARENT_METADATA_VALUE#-
crisitem.author.dept#PLACEHOLDER_PARENT_METADATA_VALUE#-
crisitem.author.dept#PLACEHOLDER_PARENT_METADATA_VALUE#-
crisitem.author.dept#PLACEHOLDER_PARENT_METADATA_VALUE#-
crisitem.author.dept#PLACEHOLDER_PARENT_METADATA_VALUE#-
crisitem.author.dept#PLACEHOLDER_PARENT_METADATA_VALUE#-
crisitem.author.dept#PLACEHOLDER_PARENT_METADATA_VALUE#-
crisitem.author.orcid#PLACEHOLDER_PARENT_METADATA_VALUE#-
crisitem.author.orcid#PLACEHOLDER_PARENT_METADATA_VALUE#-
crisitem.author.orcid#PLACEHOLDER_PARENT_METADATA_VALUE#-
crisitem.author.orcid#PLACEHOLDER_PARENT_METADATA_VALUE#-
crisitem.author.orcid#PLACEHOLDER_PARENT_METADATA_VALUE#-
crisitem.author.orcid#PLACEHOLDER_PARENT_METADATA_VALUE#-
crisitem.author.orcid#PLACEHOLDER_PARENT_METADATA_VALUE#-
crisitem.author.orcid#PLACEHOLDER_PARENT_METADATA_VALUE#-
crisitem.author.orcid#PLACEHOLDER_PARENT_METADATA_VALUE#-
crisitem.author.orcid#PLACEHOLDER_PARENT_METADATA_VALUE#-
crisitem.author.orcid#PLACEHOLDER_PARENT_METADATA_VALUE#-
crisitem.author.orcid#PLACEHOLDER_PARENT_METADATA_VALUE#-
crisitem.author.orcid#PLACEHOLDER_PARENT_METADATA_VALUE#-
crisitem.author.parentorg#PLACEHOLDER_PARENT_METADATA_VALUE#-
crisitem.author.parentorg#PLACEHOLDER_PARENT_METADATA_VALUE#-
crisitem.author.parentorg#PLACEHOLDER_PARENT_METADATA_VALUE#-
crisitem.author.parentorg#PLACEHOLDER_PARENT_METADATA_VALUE#-
crisitem.author.parentorg#PLACEHOLDER_PARENT_METADATA_VALUE#-
crisitem.author.parentorg#PLACEHOLDER_PARENT_METADATA_VALUE#-
crisitem.author.parentorg#PLACEHOLDER_PARENT_METADATA_VALUE#-
crisitem.author.parentorg#PLACEHOLDER_PARENT_METADATA_VALUE#-
Appears in Collections:Artigos de periódicos


Files in This Item:

10.1590-s1984-296120180098.pdf
Size: 1.1 MB
Format: Adobe PDF
View/Open
Show simple item record

This item is licensed under a Creative Commons License Creative Commons