Purification and biochemical characterization of TsMS 3 and TsMS 4: neuropeptide-degrading metallopeptidases in the tityus serrulatus venom

Full metadata record
DC FieldValueLanguage
dc.contributor(LBI) Laboratório de Imunoquímicapt_BR
dc.contributorLab. Bioquímicapt_BR
dc.contributor.authorCajado-Carvalho, Danielapt_BR
dc.contributor.authorSilva, Cristiane Castilho Fernandes dapt_BR
dc.contributor.authorKodama, Roberto Tadashipt_BR
dc.contributor.authorMariano, Douglas Oscar Ceolinpt_BR
dc.contributor.authorPimenta, Daniel Carvalhopt_BR
dc.contributor.authorDuzzi, Brunopt_BR
dc.contributor.authorKuniyoshi, Alexandre Kazuopt_BR
dc.contributor.authorPortaro, Fernanda Calheta Vieirapt_BR
dc.date.accessioned2020-07-09T21:23:40Z-
dc.date.available2020-07-09T21:23:40Z-
dc.date.issued2019pt_BR
dc.identifier.citationCajado-Carvalho D, Silva CCF, Kodama RT, Mariano DOC, Pimenta DC, Duzzi B, et al. Purification and biochemical characterization of TsMS 3 and TsMS 4: neuropeptide-degrading metallopeptidases in the tityus serrulatus venom. Toxins. 2019 Mar;11(4),194. doi:10.3390/toxins11040194.pt_BR
dc.identifier.urihttps://repositorio.butantan.gov.br/handle/butantan/2726-
dc.description.abstractAlthough omics studies have indicated presence of proteases on the Tityus serrulatus venom (TsV), little is known about the function of these molecules. The TsV contains metalloproteases that cleave a series of human neuropeptides, including the dynorphin A (1-13) and the members of neuropeptide Y family. Aiming to isolate the proteases responsible for this activity, the metalloserrulase 3 and 4 (TsMS 3 and TsMS 4) were purified after two chromatographic steps and identified by mass spectrometry analysis. The biochemical parameters (pH, temperature and cation effects) were determined for both proteases, and the catalytic parameters (Km, kcat, cleavage sites) of TsMS 4 over fluorescent substrate were obtained. The metalloserrulases have a high preference for cleaving neuropeptides but presented different primary specificities. For example, the Leu-enkephalin released from dynorphin A (1-13) hydrolysis was exclusively performed by TsMS 3. Neutralization assays using Butantan Institute antivenoms show that both metalloserrulases were well blocked. Although TsMS 3 and TsMS 4 were previously described through cDNA library studies using the venom gland, this is the first time that both these toxins were purified. Thus, this study represents a step further in understanding the mechanism of scorpion venom metalloproteases, which may act as possible neuropeptidases in the envenomation process.pt_BR
dc.description.sponsorship(FAPESP) Fundação de Amparo à Pesquisa do Estado de São Paulopt_BR
dc.description.sponsorship(FINEP) Financiadora de Estudos e Projetospt_BR
dc.description.sponsorship(CAPES) Coordenação de Aperfeiçoamento de Pessoal de Nível Superiorpt_BR
dc.format.extent194pt_BR
dc.language.isoEnglishpt_BR
dc.relation.ispartofToxinspt_BR
dc.rightsOpen accesspt_BR
dc.rights.urihttps://creativecommons.org/licenses/by/4.0/pt_BR
dc.titlePurification and biochemical characterization of TsMS 3 and TsMS 4: neuropeptide-degrading metallopeptidases in the tityus serrulatus venompt_BR
dc.typeArticlept_BR
dc.rights.licenseCC BYpt_BR
dc.identifier.doi10.3390/toxins11040194pt_BR
dc.identifier.urlhttps://doi.org/10.3390/toxins11040194pt_BR
dc.identifier.citationvolume11pt_BR
dc.identifier.citationissue4pt_BR
dc.subject.keywordTityus serrulatuspt_BR
dc.subject.keywordmetalloserrulasespt_BR
dc.subject.keywordproteasespt_BR
dc.subject.keywordpurificationpt_BR
dc.subject.keywordbiochemical characterizationpt_BR
dc.subject.keywordneuropeptidespt_BR
dc.relation.ispartofabbreviatedToxinspt_BR
dc.identifier.citationabntv. 11, n. 4, p. 194, mar. 2019pt_BR
dc.identifier.citationvancouver2019 Mar;11(4),194pt_BR
dc.contributor.butantanCajado-Carvalho, Daniela|:Aluno|:Lab. Imunoquímica|:PrimeiroAutor:Autor de correspondênciapt_BR
dc.contributor.butantanPimenta, Daniel Carvalho|:Pesquisador:Docente Permanente PPGTOX|:Lab. Bioquímica|:pt_BR
dc.contributor.butantanPortaro, Fernanda Calheta Vieira|:Pesquisador:Docente Permanente PPGTOX|:Lab. Imunoquímica|:Autor de correspondênciapt_BR
dc.contributor.butantanSilva, Cristiane Castilho Fernandes da|:Aluno|:Lab. Imunoquímica|:pt_BR
dc.contributor.butantanKodama, Roberto Tadashi|:Aluno|:Lab. Imunoquímica|:pt_BR
dc.contributor.butantanMariano, Douglas Oscar Ceolin|:Aluno|:Lab. Bioquímica|:pt_BR
dc.contributor.butantanDuzzi, Bruno|:Aluno|:Lab. Imunoquímica|:pt_BR
dc.contributor.butantanKuniyoshi, Alexandre Kazuo|:Aluno|:Lab. Imunoquímica|:pt_BR
dc.sponsorship.butantan(CAPES) Coordenação de Aperfeiçoamento de Pessoal de Nível Superior¦¦pt_BR
dc.sponsorship.butantanFinanciadora de Estudos e Projetos (FINEP)¦¦01.12.0450.0pt_BR
dc.sponsorship.butantan(FAPESP) Fundação de Amparo à Pesquisa do Estado de São Paulo¦¦13/15343-0pt_BR
dc.sponsorship.butantan(FAPESP) Fundação de Amparo à Pesquisa do Estado de São Paulo¦¦15/15364-3pt_BR
dc.identifier.bvsccBR78.1pt_BR
dc.identifier.bvsdbIBProdpt_BR
dc.subject.researchlineBioprospecção e desenvolvimentopt_BR
dc.description.dbindexedYespt_BR
item.grantfulltextopen-
item.languageiso639-1English-
item.fulltextCom Texto completo-
item.openairetypeArticle-
crisitem.author.dept#PLACEHOLDER_PARENT_METADATA_VALUE#-
crisitem.author.dept#PLACEHOLDER_PARENT_METADATA_VALUE#-
crisitem.author.dept#PLACEHOLDER_PARENT_METADATA_VALUE#-
crisitem.author.dept#PLACEHOLDER_PARENT_METADATA_VALUE#-
crisitem.author.dept#PLACEHOLDER_PARENT_METADATA_VALUE#-
crisitem.author.dept#PLACEHOLDER_PARENT_METADATA_VALUE#-
crisitem.author.dept#PLACEHOLDER_PARENT_METADATA_VALUE#-
crisitem.author.dept#PLACEHOLDER_PARENT_METADATA_VALUE#-
crisitem.author.orcid#PLACEHOLDER_PARENT_METADATA_VALUE#-
crisitem.author.orcid#PLACEHOLDER_PARENT_METADATA_VALUE#-
crisitem.author.orcid#PLACEHOLDER_PARENT_METADATA_VALUE#-
crisitem.author.orcid#PLACEHOLDER_PARENT_METADATA_VALUE#-
crisitem.author.orcid0000-0003-2406-0860-
crisitem.author.orcid#PLACEHOLDER_PARENT_METADATA_VALUE#-
crisitem.author.orcid#PLACEHOLDER_PARENT_METADATA_VALUE#-
crisitem.author.orcid0000-0002-9582-4699-
crisitem.journal.journalissn#PLACEHOLDER_PARENT_METADATA_VALUE#-
crisitem.journal.journaleissn#PLACEHOLDER_PARENT_METADATA_VALUE#-
Appears in Collections:Artigos de periódicos


Files in This Item:

10.3390toxins11040194.pdf
Size: 3.53 MB
Format: Adobe PDF
View/Open
Show simple item record

This item is licensed under a Creative Commons License Creative Commons