Tryptophan silver nanoparticles synthesized by photoreduction method: characterization and determination of bactericidal and anti-biofilm activities on resistant and susceptible bacteria

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dc.contributorLab. Bacteriologiapt_BR
dc.contributor.authorCourrol, Daniella dos Santospt_BR
dc.contributor.authorLopes, Carla Regina Borgespt_BR
dc.contributor.authorPereira, Camila Bueno Pachecopt_BR
dc.contributor.authorFranzolin, Marcia Reginapt_BR
dc.contributor.authorSilva, Flávia Rodrigues de Oliveirapt_BR
dc.contributor.authorCourrol, Lilia Coronatopt_BR
dc.date.accessioned2020-07-09T21:23:55Z-
dc.date.available2020-07-09T21:23:55Z-
dc.date.issued2019pt_BR
dc.identifier.citationCourrol DS, Lopes CRB, CBPP, Franzolin MR, Silva FRO, Courrol LC. Tryptophan silver nanoparticles synthesized by photoreduction method: characterization and determination of bactericidal and anti-biofilm activities on resistant and susceptible bacteria. Int. J. Tryptophan. Res.. 2019 Jan; 12. doi:10.1177/1178646919831677.pt_BR
dc.identifier.urihttps://repositorio.butantan.gov.br/handle/butantan/2745-
dc.description.abstractThe high rates of antibiotics use in hospitals have resulted in a condition where multidrug-resistant pathogens have become a severe threat to the human health worldwide. Therefore, there is an increasing necessity to identify new antimicrobial agents that can inhibit the multidrug-resistant bacteria and biofilm formation. In this study, antibacterial and anti-biofilm activities of tryptophan silver nanoparticles (TrpAgNP) were investigated. The TrpAgNPs were synthesized by photoreduction method, and the influence of irradiation time and concentration of reagents were analyzed. The nanoparticles were characterized by transmission electron microscopy, Zeta Potential and (UV)-absorption spectra. The antibacterial activity of TrpAgNPs was tested for antibiotic-resistant and susceptible pathogens, Staphylococcus aureus, Staphylococcus epidermidis, Escherichia coli, Citrobacter freundii, Klebsiella pneumoniae, Salmonella typhimurium, and Pseudomonas aeruginosa, evaluating the influence of photoreduction parameters in bactericidal effect. The results have shown that TrpAgNPs solutions with lower tryptophan/silver nitrate (AgNO3) ratio and higher AgNO3 concentration have higher bactericidal action against bacteria with inhibition of ~100% in almost all studied bacterial strains. The antimicrobial activity of TrpAgNPs within biofilms generated under static conditions of antibiotic-resistant and susceptible strains of S. aureus, S. epidermidis, E. coli, K. pneumoniae, C. freundii, and P. aeruginosa was also investigated. The results showed that TrpAgNPs have an inhibitory effect against biofilm formation, exceeding 50% in the case of Gram-negative bacteria (E. coli, K. pneumoniae, C. freundii, and P. aeruginosa—54.8% to 98.8%). For Gram-positive species, an inhibition of biofilm formation of 68.7% to 72.2 % was observed for S. aureus and 20.0% to 40.2% for S. epidermidis.pt_BR
dc.description.sponsorshipFundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)pt_BR
dc.description.sponsorshipConselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq)pt_BR
dc.format.extentp.pt_BR
dc.language.isoEnglishpt_BR
dc.relation.ispartofInternational Journal of Tryptophan Researchpt_BR
dc.rightsOpen Accesspt_BR
dc.titleTryptophan silver nanoparticles synthesized by photoreduction method: characterization and determination of bactericidal and anti-biofilm activities on resistant and susceptible bacteriapt_BR
dc.typeArticlept_BR
dc.identifier.doi10.1177/1178646919831677pt_BR
dc.identifier.urlhttps://doi.org/10.1177/1178646919831677pt_BR
dc.contributor.externalInstituto de Pesquisas Energéticas e Nucleares (IPEN)¦¦Brasilpt_BR
dc.contributor.externalUniversidade Federal de São Paulo (UNIFESP)¦¦Brasilpt_BR
dc.identifier.citationvolume12pt_BR
dc.subject.keywordtryptophanpt_BR
dc.subject.keywordsilver nanoparticlespt_BR
dc.subject.keywordphotoreductionpt_BR
dc.subject.keywordantimicrobial activitypt_BR
dc.subject.keywordanti-biofilmpt_BR
dc.relation.ispartofabbreviatedInt J Tryptophan Respt_BR
dc.identifier.citationabntv. 12, jan. 2019pt_BR
dc.identifier.citationvancouver2019 Jan; 12pt_BR
dc.contributor.butantanCourrol, Daniella dos Santos|:Aluno|:Lab. Bacteriologia|:PrimeiroAutorpt_BR
dc.contributor.butantanPereira, Camila Bueno Pacheco|:Técnico|:Lab. Bacteriologia|:pt_BR
dc.contributor.butantanFranzolin, Marcia Regina|:Pesquisador|:Lab. Bacteriologia|:pt_BR
dc.sponsorship.butantanConselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq)¦¦pt_BR
dc.sponsorship.butantanFundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)¦¦2014/06960-9pt_BR
dc.identifier.bvsccBR78.1pt_BR
dc.identifier.bvsdbIBProdpt_BR
dc.description.dbindexedYespt_BR
item.openairetypeArticle-
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item.grantfulltextopen-
item.languageiso639-1English-
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